Cytokeratin 1 (KRT1) antibody
| Antigen |
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| Synonyms |
K1, CK1, EHK, EHK1, EPPK, KRT1A, NEPPK |
| Clonality |
Polyclonal |
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Host
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Reactivity
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Application
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Alternatives Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
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| Catalog no. |
ABIN138945 |
| Quantity |
6 ml (Variants) |
| Price |
Product not available in this region.
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| Shipping to |
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Alternative name
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Cytokeratin 1
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Immunogen
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BALB/C mice were immunized with crude extract of splenic cells from a nude mouse engrafted with MCF-7 cells.
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Isotype
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IgG1 (Matching secondary antibodies)
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Description
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This antibody recognizes keratin polypeptide of 45, 46 and 56.5 kD. It shows a broad pattern of reactivity with human epithelial tissues from simple glandular epithelia to stratified squamous epithelia, like epidermis, mammary gland ducts and tracheal epithelium.
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Application Notes
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This antibody has been pretitered and quality controlled to work on formalin-fixed paraffin-embedded as well as acetone fixed cryostat tissue sections. No further titration is required. We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Enzymatic predigestion of paraffin embedded tissues is recommended prior to the immunostaining.
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Buffer
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PBS, pH7.2 with 0.05% sodium azide as preservative.
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Storage
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Store the antibody at 4-8 C. Do not use it beyond the expiration date stated on the label.
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Research Area
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Signaling, Cytoskeleton, Cytokeratins, Extracellular Matrix
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Restrictions
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For Research Use only
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Alternatives for antigen "Cytokeratin 1 (KRT1)", type "Antibodies"
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Hosts
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Mouse (29), Guinea Pig (2), Rabbit (2)
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Reactivities
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Human (28), Cow (Bovine) (3), Rat (Rattus) (3), Chicken (2), Frog (2), Snake (2), Mouse (Murine) (1)
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Applications
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Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (18), Western Blotting (WB) (11), Immunohistochemistry (Frozen Sections) (IHC (fro)) (9), Immunohistochemistry (IHC) (6), Enzyme Immunoassay (EIA) (4), Immunofluorescence (IF) (2), ELISA (1), Immunocytochemistry (ICC) (1)
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