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Actin, Alpha, gamma antibody

Details for Product No. ABIN139058, Supplier: Login to see
Clonality (Clone)
Monoclonal ()
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
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Immunogen Muscle actin, purified from human myocardium
Clone HHF35
Isotype IgG1
Specificity Recognises muscle specific actin, a 42kD protein specific for actin present in skeletal, cardiac and smooth muscle. Clone HHF35 is specific for alpha actin and does not recognise beta actin. This antibody may be useful in the study of tumours arising from these muscle cells, such as rhabdomyosarcomas, leiomyomas and leiomyosarcomas.
Cross-Reactivity Mouse (Murine), Monkey, Rat (Rattus)
Purification Unpurified. Hybridoma culture supernatant.
Alternative Name Actin (pan alpha / gamma 2)
Background The protein encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Defects in this gene cause aortic aneurysm familial thoracic type 6. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq]
Synonyms: AAT6, ACTSA
Research Area Extracellular Matrix
Application Notes For IHC-Fr: Use this product at 1:10-1:40. For IHC-P: Use this product at 1:10-1:40. This product does not require protein digestion pre-treatment of paraffin sections. This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections. Not tested in other applications. Optimal dilutions/concentrations should be determined by the researcher.

Positive Controls: Skeletal tissue

Restrictions For Research Use only
Format Liquid
Buffer TCS containing PBS, 1% BSA and 0.09% sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze
Storage 4 °C
Storage Comment Keep as concentrated solution, aliquot and store at 4°C.
Background publications Tsukada, Tippens, Gordon et al.: "HHF35, a muscle-actin-specific monoclonal antibody. I. Immunocytochemical and biochemical characterization." in: The American journal of pathology, Vol. 126, Issue 1, pp. 51-60, 1987 (PubMed).

Giffhorn, Gottschalk: "Inactivation of citrate lyase from Rhodopseudomonas gelatinosa by a specific deacetylase and inhibition of this inactivation by L-(+1-glutamate." in: Journal of bacteriology, Vol. 124, Issue 3, pp. 1052-61, 1976 (PubMed).