Actin, Alpha, gamma antibody

Antigen: Actin, Alpha, gamma

Clonality: Monoclonal

Host: Mouse

Reactivity: Dog (Canine)

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC)

Catalog no.: ABIN139058

Additional Information

Alternative name: Actin (pan alpha / gamma 2)

Immunogen: Muscle actin, purified from human myocardium

Cross-Reactivity: Dog (Canine), Chicken, Cat (Feline), Monkey, Mouse (Murine), Rat (Rattus)

Description: Mouse Antibody to Human Actin ( Muscle )

Specificity: Recognises muscle specific actin, a 42kD protein specific for actin present in skeletal, cardiac and smooth muscle. Clone HHF35 is specific for alpha actin and does not recognise beta actin. This antibody may be useful in the study of tumours arising from

Application Details

Application Notes: For IHC-Fr: Use this product at 1:10-1:40. For IHC-P: Use this product at 1:10-1:40. This product does not require protein digestion pre-treatment of paraffin sections. This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections. Not tested in other applications. Optimal dilutions/concentrations should be determined by the researcher.

Concentration: Not determined

Purification: Unpurified

Buffer: TCS containing PBS, 1% BSA and 0.09% sodium azide

Storage: Keep as concentrated solution, aliquot and store at 4C. Do not freeze.

Research Area: Extracellular Matrix

Restrictions: For Research Use only

Publications

Giffhorn, Gottschalk: "Inactivation of citrate lyase from Rhodopseudomonas gelatinosa by a specific deacetylase and inhibition of this inactivation by L-(+1-glutamate." in: Journal of bacteriology, Vol. 124, Issue 3, pp. 1052-61, 1976 (PubMed).

Tsukada, Tippens, Gordon et al.: "HHF35, a muscle-actin-specific monoclonal antibody. I. Immunocytochemical and biochemical characterization." in: The American journal of pathology, Vol. 126, Issue 1, pp. 51-60, 1987 (PubMed).