SHC2 antibody (AA 161-270) (Alexa Fluor 350)

Catalog No. ABIN1395417

Product Details anti-SHC2 Antibody (Alexa Fluor 350)

Target: SHC2 (SHC (Src Homology 2 Domain Containing) Transforming Protein 2 (SHC2))

Binding Specificity: AA 161-270

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SHC2 antibody is conjugated to Alexa Fluor 350

Application: Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc))

Predicted Reactivity: Human,Mouse,Rat,Dog,Cow,Sheep

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human SHC2/Sck

Isotype: IgG

Application Details

Application Notes: IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for SHC2

Target: SHC2 (SHC (Src Homology 2 Domain Containing) Transforming Protein 2 (SHC2))

Alternative Name: SHC2/Sck (SHC2 Products)

Synonyms: SCK antibody, SHCB antibody, SLI antibody, 6720466E06 antibody, ShcB antibody, Sli antibody, SHC adaptor protein 2 antibody, SHC (Src homology 2 domain containing) transforming protein 2 antibody, SHC2 antibody, Shc2 antibody

Background:

Synonyms: Protein Sck, SCK, SH2 domain protein C2, SHC transforming protein 2, SHCB, Src homology 2 domain containing transforming protein C2, SHC2_HUMAN.

Background: Src homology 2 (SH2) domains bind specifically to tyrosine-phosphorylated proteins that temporally participate in signal transduction events (1). Shc-like protein (Sck) is a neuronal adaptor protein that contains an N-terminal PTB (phosphotyrosine binding) domain, a collagen homology (CH) domain, and a conserved C-terminal SH2 domain (2,3). Human Sck transcripts are present at high levels in liver, pancreas, prostate and ovary (4,5). In vascular endothelial cells, Sck participates in VEGF-induced signal transduction (6). Treatment of human umbilical vein endothelial (HUVEC) cells with VEGF induces recruitment of Sck to tyrosine-1175 of the kinase insert domain-containing receptor (KDR) and enhances Sck tyrosine phosphorylation (7,8).

Pathways: RTK Signaling, Neurotrophin Signaling Pathway, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals, VEGF Signaling