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Collagen, Type III (COL3) (Chain alpha 1) antibody

Details for Product No. ABIN139816, Supplier: Login to see
Antigen
Epitope
Chain alpha 1
13
13
5
3
2
1
1
1
1
Reactivity
Human
161
70
51
12
7
3
3
2
1
1
1
1
Host
Mouse
139
27
27
1
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
19
5
4
3
3
3
3
3
3
3
3
3
1
1
1
Application
Dot Blot (DB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Western Blotting (WB)
127
82
52
50
48
41
33
26
23
18
13
7
3
2
1
1
1
1
Supplier
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Immunogen full length native protein human collagen type III
Clone FH-7A
Isotype IgG1
Specificity The antibody specifically recognizes native and denatured collagen type III. It does not recognise collagen types I, II, IV, V, VI and X.
Cross-Reactivity Rat (Rattus)
Characteristics Cellular Localization: Extracellular matrix
Purification Ascites.
Alternative Name Collagen type III
Background Collagen III is a fibrillar collagen that is found in extensible connective tissues such as skin, lung, and the vascular system, frequently in association with type I Collagen. Mutations in the gene are associated with Ehlers Danlos syndrome type IV, and with aortic and arterial aneurysms. Although alternate transcripts have been detected for this gene, they are the result of mutations, these mutations alter splicing, often leading to the exclusion of multiple exons.
Synonyms: Collagen III, EDS4A, P02461, 1281, 120180, COL3A1
Pathways
Application Notes For IHC-Fr: Use at a dilution of 1:4,000. WB: 1/100-1/1000. The antibody recognizes a ~ 70 kDa band in reduced denatured preps and a ~ 300 kDa band in non-reduced, non-denatured preps. Not tested in other applications. Optimal dilutions/concentrations should be determined by the researcher.
Comment

Positive Controls: IHC: Human skin sections WB: recombinant collagen III

Restrictions For Research Use only
Format Liquid
Buffer Contains 15 mM sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Store at -20°C. For prolonged storage, freeze in working aliquots.
Supplier Images
Immunohistochemistry (IHC) image for anti-Collagen, Type III (COL3) (Chain alpha 1) antibody (ABIN139816) anti-Collagen, Type III (COL3) (Chain alpha 1) antibody
Immunohistochemistry (IHC) image for anti-Collagen, Type III (COL3) (Chain alpha 1) antibody (ABIN139816) anti-Collagen, Type III (COL3) (Chain alpha 1) antibody (Image 2)
Background publications Mayne: "Preparation and applications of monoclonal antibodies to different collagen types." in: Clinical biochemistry, Vol. 21, Issue 2, pp. 111-5, 1988 (PubMed).

Superti-Furga, Steinmann, Ramirez et al.: "Molecular defects of type III procollagen in Ehlers-Danlos syndrome type IV." in: Human genetics, Vol. 82, Issue 2, pp. 104-8, 1989 (PubMed).

Sanes, Engvall, Butkowski et al.: "Molecular heterogeneity of basal laminae: isoforms of laminin and collagen IV at the neuromuscular junction and elsewhere." in: The Journal of cell biology, Vol. 111, Issue 4, pp. 1685-99, 1990 (PubMed).