Collagen, Type III (COL3) (Chain alpha 1) antibody

Details for Product No. ABIN139816
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Antigen
Epitope
Chain alpha 1
(5), (4), (2), (1), (1), (1)
Reactivity
Human
(124), (69), (52), (45), (26), (16), (13), (2), (2), (1), (1)
Host
Mouse
(109), (25), (14), (1)
Clonality
Monoclonal
Conjugate
Un-conjugated
(14), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3)
Application
Dot Blot (DB), Western Blotting (WB), Immunohistochemistry (IHC), ELISA
(103), (59), (57), (45), (38), (30), (25), (24), (18), (12), (7), (2), (1), (1)
Pubmed 3 references available
Catalog no. ABIN139816
Quantity 0.1 mL
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Immunogen Human collagen type III
Isotype IgG1
Specificity The antibody specifically recognizes native and denatured collagen type III. It does not recognise collagen types I, II, IV, V, VI and X.
Cross-Reactivity Rat (Rattus)
Purification Ascites
Alternative Name Collagen type III
Background Mouse monoclonal [FH-7A] to Collagen III
Application Notes For IHC-Fr: Use at a dilution of 1:4,000. WB: 1/100-1/1000. The antibody recognizes a ~ 70 kDa band in reduced denatured preps and a ~ 300 kDa band in non-reduced, non-denatured preps. Not tested in other applications. Optimal dilutions/concentrations should be determined by the researcher.
Restrictions For Research Use only
Format Liquid
Concentration Raw ascites
Preservative Sodium azide
General Sanes, Engvall, Butkowski et al.: "Molecular heterogeneity of basal laminae: isoforms of laminin and collagen IV at the neuromuscular junction and elsewhere." in: The Journal of cell biology, Vol. 111, Issue 4, pp. 1685-99, 1990 (PubMed).

Superti-Furga, Steinmann, Ramirez et al.: "Molecular defects of type III procollagen in Ehlers-Danlos syndrome type IV." in: Human genetics, Vol. 82, Issue 2, pp. 104-8, 1989 (PubMed).

Mayne: "Preparation and applications of monoclonal antibodies to different collagen types." in: Clinical biochemistry, Vol. 21, Issue 2, pp. 111-5, 1988 (PubMed).

Hosts (109), (25), (14), (1)
Reactivities (124), (69), (52), (45), (26), (16), (13), (2), (2), (1), (1)
Applications (103), (59), (57), (45), (38), (30), (25), (24), (18), (12), (7), (2), (1), (1)
Conjugates (14), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3)
Epitopes (5), (4), (2), (1), (1), (1)
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