UBE2O antibody (AA 1201-1292) (Alexa Fluor 350)

Catalog No. ABIN1398747

Product Details anti-UBE2O Antibody (Alexa Fluor 350)

Target: UBE2O (Ubiquitin-Conjugating Enzyme E2O (UBE2O))

Binding Specificity: AA 1201-1292

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This UBE2O antibody is conjugated to Alexa Fluor 350

Application: Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Predicted Reactivity: Mouse,Rat,Pig,Horse,Chicken

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human UBE2O/E2 230K

Isotype: IgG

Application Details

Application Notes: IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for UBE2O

Target: UBE2O (Ubiquitin-Conjugating Enzyme E2O (UBE2O))

Alternative Name: UBE2O (UBE2O Products)

Synonyms: e2-230k antibody, E2-230K antibody, 9630022H21 antibody, B230113M03Rik antibody, mKIAA1734 antibody, RGD1310297 antibody, ubiquitin conjugating enzyme E2 O antibody, ubiquitin-conjugating enzyme E2O antibody, UBE2O antibody, ube2o antibody, Ube2o antibody

Background:

Synonyms: E2 230K, ubiquitin-conjugating enzyme E2O, EC=6.3.2.19, FLJ12878, KIAA1734, Likely Ortholog of Mouse Ubiquitin Conjugating Enzyme, RP23 193A16.5, UBE2O, UBE2O_HUMAN, Ubiquitin carrier protein O, Ubiquitin conjugating enzyme E2 O, ubiquitin conjugating enzyme E2O.

Background: UBE20, also known as E2-230K, is a 1,292 amino acid member of the uniquitin-conjugating enzyme family that is involved in protein modification. Expressed predominately in heart and skeletal muscle, UBE2O functions to catalyze the ATP-dependent covalent attachment of ubiquitin to select proteins, thereby targeting the ubiquitinated proteins for proteasomal degradation. The gene encoding UBE2O maps to human chromosome 17, which comprises over 2.5 % of the human genome and encodes over 1,200 genes. Two key tumor suppressor genes are associated with chromosome 17, namely, p53 and BRCA1. Tumor suppressor p53 is necessary for maintenance of cellular genetic integrity by moderating cell fate through DNA repair versus cell death. Malfunction or loss of p53 expression is associated with malignant cell growth and Li-Fraumeni syndrome. Like p53, BRCA1 is directly involved in DNA repair, though specifically it is recognized as a genetic determinant of early onset breast cancer and predisposition to cancers of the ovary, colon, prostate gland and fallopian tubes.

Gene ID: 63893