ZFHX3 antibody (AA 301-400) (HRP)

Catalog No. ABIN1411322

Product Details anti-ZFHX3 Antibody (HRP)

Target: ZFHX3 (Zinc Finger Homeobox 3 (ZFHX3))

Binding Specificity: AA 301-400

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ZFHX3 antibody is conjugated to HRP

Application: ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Cross-Reactivity: Rat

Predicted Reactivity: Human,Mouse,Dog,Cow,Sheep,Horse,Chicken,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human ATBF1

Isotype: IgG

Application Details

Application Notes: IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for ZFHX3

Target: ZFHX3 (Zinc Finger Homeobox 3 (ZFHX3))

Alternative Name: ATBF1 (ZFHX3 Products)

Synonyms: ZFHX3 antibody, ATBF1 antibody, ATBT antibody, ZNF927 antibody, A230102L03Rik antibody, Atbf1 antibody, WBP9 antibody, mKIAA4228 antibody, RGD1560268 antibody, zax-2 antibody, zinc finger homeobox 3 antibody, AT-binding transcription factor1 antibody, zfhx3 antibody, ZFHX3 antibody, ATBF1/ZFHX4b antibody, Zfhx3 antibody

Background:

Synonyms: Alpha fetoprotein enhancer binding protein, AT binding transcription factor 1, AT mot binding factor, ATBT, ZFHX3, ZFHX3_HUMAN.

Background: AT-motif binding factor 1 (ATBF1) binds to the AT-rich core sequence element in the human a-fetoprotein enhancer (1). Alternative splicing generates the ATBF1-A and ATBF1-B (2,3). While ATBF1-A contains a 920-amino acid extension at the N-terminus, both ATBF1-A and ATBF1-B contain 4 DNA-binding homeobox domains (2,3). Additionally, ATBF1-A contains 23 zinc finger motifs while ATBF1-B contains 18 zinc finger motifs (13). The N-terminal extension unique to ATBF1-A has transcriptional repressor activity (4). In the small intestine, ATBF1-A inhibits expression of the brushborder enzyme aminopeptidase-N through direct binding to the AT motif element (5). Besides functioning in transcription regulation, ATBF1 also functions in ATPase activity (6). ATPase activity associated with ATBF1-A is DNA/RNA-dependent and requires both homeobox domains and zinc finger motifs (6). ATBF1 is highly expressed in spleen and brain tissues (7). The gene encoding human ATBF1 maps to chromosome 16q22.3-q23.1 (8).

Pathways: Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development