PPP1R9B antibody (AA 358-460) (HRP)

Catalog No. ABIN1413326

Product Details anti-PPP1R9B Antibody (HRP)

Target: PPP1R9B (Protein Phosphatase 1, Regulatory Subunit 9B, Spinophilin (PPP1R9B))

Binding Specificity: AA 358-460

Reactivity: Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PPP1R9B antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Cross-Reactivity: Mouse, Rat

Predicted Reactivity: Human,Dog,Sheep,Pig

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Spinophilin/Neurabin 2

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for PPP1R9B

Target: PPP1R9B (Protein Phosphatase 1, Regulatory Subunit 9B, Spinophilin (PPP1R9B))

Alternative Name: Spinophilin/Neurabin 2 (PPP1R9B Products)

Synonyms: Neb2 antibody, PPP1R6 antibody, PPP1R9 antibody, SPINO antibody, Spn antibody, SPL antibody, protein phosphatase 1, regulatory subunit 9B antibody, protein phosphatase 1 regulatory subunit 9B antibody, Ppp1r9b antibody, PPP1R9B antibody

Background:

Synonyms: Spn, SPINO, PPP1R6, PPP1R9, Neurabin-2, Neurabin-II, Protein phosphatase 1 regulatory subunit 9B, Spinophilin, PPP1R9B

Background: Seems to act as a scaffold protein in multiple signaling pathways. Modulates excitatory synaptic transmission and dendritic spine morphology. Binds to actin filaments (F-actin) and shows cross-linking activity. Binds along the sides of the F-actin. May play an important role in linking the actin cytoskeleton to the plasma membrane at the synaptic junction. Believed to target protein phosphatase 1/PP1 to dendritic spines, which are rich in F-actin, and regulates its specificity toward ion channels and other substrates, such as AMPA-type and NMDA-type glutamate receptors. Plays a role in regulation of G-protein coupled receptor signaling, including dopamine D2 receptors and alpha-adrenergic receptors. May establish a signaling complex for dopaminergic neurotransmission through D2 receptors by linking receptors downstream signaling molecules and the actin cytoskeleton. Binds to ADRA1B and RGS2 and mediates regulation of ADRA1B signaling. May confer to Rac signaling specificity by binding to both, RacGEFs and Rac effector proteins. Probably regulates p70 S6 kinase activity by forming a complex with TIAM1 (By similarity). Required for hepatocyte growth factor (HGF)-induced cell migration.

Gene ID: 84687

UniProt: Q96SB3

Pathways: Regulation of G-Protein Coupled Receptor Protein Signaling