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|+1 404 474 4654|
|+1 888 205 9894 (TF)|
LSM1 Homolog, U6 Small Nuclear RNA Associated (S. Cerevisiae) (LSM1) antibody
|Synonyms||lsm1, Lsm1, NV45, CASM, YJL124C, 2810025O06Rik|
Alternatives Western Blotting (WB)
|8 references available|
|Quantity||50 µg (1 mg/ml)|
|Price||Product not available in this region.|
|Immunogen||Full length native protein (purified) (Human) LSM1.|
|Cross-Reactivity||Human, Mouse (Murine), Rat (Rattus)|
|Description||SM-like proteins were identified in a variety of organisms based on sequence homology with the SM protein family. SM-like proteins contain the SM sequence motif, which consists of 2 regions separated by a linker of variable length that folds as a loop. The SM-like proteins are thought to form a stable heteromer present in tri-snRNP particles, which are important for pre-mRNA splicing.|
|Specificity||Binds specifically to the 3-terminal U-tract of U6 snRNA LSm subunits form a heteromer with a donut shape.|
|Application Notes||Recommended dilutions - WB: 1/2000. Predicted molecular weight: 15 kDa. We recommend using about 2.5 ug of positive control protein in the colorimetric method, and about 10x less for ECL detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.|
|Purification||Immunogen affinity purified|
|Storage||Aliquot and store at -20C. Avoid freeze / thaw cycles|
|Research Area||Chromatin and Nuclear Signaling, DNA/RNA|
|Restrictions||For Research Use only|
|anti-LSM1 Homolog, U6 Small Nuclear RNA Associated (S. Cerevisiae) (LSM1) antibody|
Schweinfest, Graber, Chapman et al.: "CaSm: an Sm-like protein that contributes to the transformed state in cancer cells." in: Cancer research, Vol. 57, Issue 14, pp. 2961-5, 1997 (PubMed).
Salgado-Garrido, Bragado-Nilsson, Kandels-Lewis et al.: "Sm and Sm-like proteins assemble in two related complexes of deep evolutionary origin." in: The EMBO journal, Vol. 18, Issue 12, pp. 3451-62, 1999 (PubMed).
Eystathioy, Peebles, Hamel et al.: "Autoantibody to hLSm4 and the heptameric LSm complex in anti-Sm sera." in: Arthritis and rheumatism, Vol. 46, Issue 3, pp. 726-34, 2002 (PubMed).
Takahashi, Suzuki, Inaguma et al.: "Down-regulation of Lsm1 is involved in human prostate cancer progression." in: British journal of cancer, Vol. 86, Issue 6, pp. 940-6, 2002 (PubMed).
Strausberg, Feingold, Grouse et al.: "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 26, pp. 16899-903, 2002 (PubMed).
Ingelfinger, Arndt-Jovin, Luehrmann et al.: "The human LSm1-7 proteins colocalize with the mRNA-degrading enzymes Dcp1/2 and Xrnl in distinct cytoplasmic foci." in: RNA (New York, N.Y.), Vol. 8, Issue 12, pp. 1489-501, 2003 (PubMed).
Lehner, Semple, Brown et al.: "Analysis of a high-throughput yeast two-hybrid system and its use to predict the function of intracellular proteins encoded within the human MHC class III region." in: Genomics, Vol. 83, Issue 1, pp. 153-67, 2003 (PubMed).
Lehner, Sanderson: "A protein interaction framework for human mRNA degradation." in: Genome research, Vol. 14, Issue 7, pp. 1315-23, 2004 (PubMed).
|Hosts||Rabbit (12), Mouse (5), Chicken (1)|
|Reactivities||Human (18), Dog (Canine) (3), Mouse (Murine) (3), Chicken (2), Cow (Bovine) (2), Rat (Rattus) (2), Cat (Feline) (1), Xenopus laevis (1), Zebrafish (1)|
|Applications||Western Blotting (WB) (18), ELISA (10), Immunohistochemistry (IHC) (6), Immunofluorescence (IF) (5), Flow Cytometry (FACS) (4), Immunocytochemistry (ICC) (1), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (1), Immunoprecipitation (IP) (1)|
|Epitopes||C-Term (2), Internal Region (2), AA 1-133 (1), Center (1), N-Term (1)|