LSM1 Homolog, U6 Small Nuclear RNA Associated (S. Cerevisiae) (LSM1) antibody

Antigen: LSM1 Homolog, U6 Small Nuclear RNA Associated (S. Cerevisiae) (LSM1)

Synonyms: lsm1, Lsm1, NV45, CASM, YJL124C, 2810025O06Rik

Clonality: Polyclonal

Host: Chicken

Reactivity: Human

Application: Western Blotting (WB)

Catalog no.: ABIN141457

Additional Information

Alternative name: LSM1

Immunogen: Full length native protein (purified) (Human) LSM1.

Cross-Reactivity: Human, Mouse (Murine), Rat (Rattus)

Description: SM-like proteins were identified in a variety of organisms based on sequence homology with the SM protein family. SM-like proteins contain the SM sequence motif, which consists of 2 regions separated by a linker of variable length that folds as a loop. The SM-like proteins are thought to form a stable heteromer present in tri-snRNP particles, which are important for pre-mRNA splicing.

Specificity: Binds specifically to the 3-terminal U-tract of U6 snRNA LSm subunits form a heteromer with a donut shape.

Application Details

Application Notes: Recommended dilutions - WB: 1/2000. Predicted molecular weight: 15 kDa. We recommend using about 2.5 ug of positive control protein in the colorimetric method, and about 10x less for ECL detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

Concentration: 1 mg/ml

Purification: Immunogen affinity purified

Buffer: PBS

Preservative: None

Storage: Aliquot and store at -20C. Avoid freeze / thaw cycles

Research Area: Chromatin and Nuclear Signaling, DNA/RNA

Restrictions: For Research Use only

Images

anti-LSM1 Homolog, U6 Small Nuclear RNA Associated (S. Cerevisiae) (LSM1) antibody

Publications

Schweinfest, Graber, Chapman et al.: "CaSm: an Sm-like protein that contributes to the transformed state in cancer cells." in: Cancer research, Vol. 57, Issue 14, pp. 2961-5, 1997 (PubMed).

Salgado-Garrido, Bragado-Nilsson, Kandels-Lewis et al.: "Sm and Sm-like proteins assemble in two related complexes of deep evolutionary origin." in: The EMBO journal, Vol. 18, Issue 12, pp. 3451-62, 1999 (PubMed).

Eystathioy, Peebles, Hamel et al.: "Autoantibody to hLSm4 and the heptameric LSm complex in anti-Sm sera." in: Arthritis and rheumatism, Vol. 46, Issue 3, pp. 726-34, 2002 (PubMed).

Takahashi, Suzuki, Inaguma et al.: "Down-regulation of Lsm1 is involved in human prostate cancer progression." in: British journal of cancer, Vol. 86, Issue 6, pp. 940-6, 2002 (PubMed).

Strausberg, Feingold, Grouse et al.: "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 26, pp. 16899-903, 2002 (PubMed).

Ingelfinger, Arndt-Jovin, Luehrmann et al.: "The human LSm1-7 proteins colocalize with the mRNA-degrading enzymes Dcp1/2 and Xrnl in distinct cytoplasmic foci." in: RNA (New York, N.Y.), Vol. 8, Issue 12, pp. 1489-501, 2003 (PubMed).

Lehner, Semple, Brown et al.: "Analysis of a high-throughput yeast two-hybrid system and its use to predict the function of intracellular proteins encoded within the human MHC class III region." in: Genomics, Vol. 83, Issue 1, pp. 153-67, 2003 (PubMed).

Lehner, Sanderson: "A protein interaction framework for human mRNA degradation." in: Genome research, Vol. 14, Issue 7, pp. 1315-23, 2004 (PubMed).