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MRE11 Meiotic Recombination 11 Homolog A (S. Cerevisiae) (MRE11A) antibody

Details for Product No. ABIN141602
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Antigen
Synonyms wu:faa63d12, zgc:64018, Mre11, Mre11b, ATLD, HNGS1, MRE11, MRE11B
Reactivity
Human
(107), (59), (56), (27), (13), (4), (2), (1), (1), (1), (1), (1)
Host
Rabbit
(101), (16)
Clonality
Polyclonal
Conjugate
Un-conjugated
(3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB)
(97), (52), (21), (20), (20), (12), (7), (5), (2), (1), (1), (1)
Pubmed 12 references available
Quantity 50 µL
Shipping to United States (Change)
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Catalog No. ABIN141602
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Immunogen Human Mre11 full length.
Specificity This antibody is specific for human Mre11 (full length).
Cross-Reactivity Mouse (Murine)
Purification Whole antisera.
Alternative Name MRE11
Background The hMre11-hRad50-NBS1 protein plays a central role in the human cellular DNA-Damage response, with recent studies indicating that these proteins help link DNA-damage detection to DNA-repair and cell cycle-checkpoint functions. This protein complex has been implicated in the activation of cell cycle-regulatory pathways, due to the fact that the NBS1 gene mutates in the chromosomal-instability syndrome, Nijmegen breakdown syndrome (NBS), which is characterized by increased cancer incidence, cell cycle checkpoint defects, and ionizing radiation sensitivity. hMre11-hRad50 foci form in response to DNA double-strand breaks and rely upon a DNA damage-induced signaling pathway. However, hMre11 migrates to sites of damage while hRad51 does not localize at these sites. These findings are consistent with the distinct role of these proteins in DNA repair.
Synonyms: MRE11, MRE11B, P49959, 600814, ATLD, 4361, MRE11A, HNGS1
Application Notes By Western blot, this antibody recognizes a band at 81 kDa representing hMre11 in TK6 or other human cells. By immunofluorescence, this antibody has been used with methanol-fixed IMR90 primary human fibroblasts. Immunoprecipitation tests have been done with 3-4 X 10^6 cells. Suggested working dilutions: Western Blot: 1:10,000 in human and mouse Immunofluorescence: 1:200 in human (has not been tested in mouse) Immunoprecipitation: 3 ul for 3 x 10-6 cells (in human and mouse)
Comment

Positive Controls: Hela whole cell lysate

Restrictions For Research Use only
Format Liquid
Buffer 0.02% sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Keep as concentrated solution, aliquot and store at 4°C.
Supplier Images
anti-MRE11 Meiotic Recombination 11 Homolog A (S. Cerevisiae) (MRE11A) antibody anti-MRE11 Meiotic Recombination 11 Homolog A (S. Cerevisiae) (MRE11A) antibody
General Wang, Cortez, Yazdi et al.: "BASC, a super complex of BRCA1-associated proteins involved in the recognition and repair of aberrant DNA structures." in: Genes & development, Vol. 14, Issue 8, pp. 927-39, 2000 (PubMed).

Limoli, Giedzinski, Morgan et al.: "Inaugural article: polymerase eta deficiency in the xeroderma pigmentosum variant uncovers an overlap between the S phase checkpoint and double-strand break repair." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 97, Issue 14, pp. 7939-46, 2000 (PubMed).

Tauchi, Kobayashi, Morishima et al.: "The forkhead-associated domain of NBS1 is essential for nuclear foci formation after irradiation but not essential for hRAD50[middle dot]hMRE11[middle dot]NBS1 complex DNA repair activity." in: The Journal of biological chemistry, Vol. 276, Issue 1, pp. 12-5, 2001 (PubMed).

Carbone, Pearson, Minucci et al.: "PML NBs associate with the hMre11 complex and p53 at sites of irradiation induced DNA damage." in: Oncogene, Vol. 21, Issue 11, pp. 1633-40, 2002 (PubMed).

Franchitto, Pichierri: "Bloom's syndrome protein is required for correct relocalization of RAD50/MRE11/NBS1 complex after replication fork arrest." in: The Journal of cell biology, Vol. 157, Issue 1, pp. 19-30, 2002 (PubMed).

Brown, Franco, Burkle et al.: "Role of poly(ADP-ribosyl)ation in DNA-PKcs- independent V(D)J recombination." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 7, pp. 4532-7, 2002 (PubMed).

Kobayashi, Tauchi, Sakamoto et al.: "NBS1 localizes to gamma-H2AX foci through interaction with the FHA/BRCT domain." in: Current biology : CB, Vol. 12, Issue 21, pp. 1846-51, 2002 (PubMed).

Her, Vo, Wu: "Evidence for a direct association of hMRE11 with the human mismatch repair protein hMLH1." in: DNA repair, Vol. 1, Issue 9, pp. 719-29, 2003 (PubMed).

Cleaver, Bartholomew, Char et al.: "Polymerase eta and p53 jointly regulate cell survival, apoptosis and Mre11 recombination during S phase checkpoint arrest after UV irradiation." in: DNA repair, Vol. 1, Issue 1, pp. 41-57, 2003 (PubMed).

Cerosaletti, Concannon: "Nibrin forkhead-associated domain and breast cancer C-terminal domain are both required for nuclear focus formation and phosphorylation." in: The Journal of biological chemistry, Vol. 278, Issue 24, pp. 21944-51, 2003 (PubMed).

Donahue, Lundberg, Saplis et al.: "Deficient regulation of DNA double-strand break repair in Fanconi anemia fibroblasts." in: The Journal of biological chemistry, Vol. 278, Issue 32, pp. 29487-95, 2003 (PubMed).

Siwicki, Degerman, Chrzanowska et al.: "Telomere maintenance and cell cycle regulation in spontaneously immortalized T-cell lines from Nijmegen breakage syndrome patients." in: Experimental cell research, Vol. 287, Issue 1, pp. 178-89, 2003 (PubMed).

Validation Images
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