NFAT5 antibody (pSer1197) (Cy5)

Catalog No. ABIN1420794

Product Details anti-NFAT5 Antibody (Cy5)

Target: NFAT5 (Nuclear Factor of Activated T-Cells 5, Tonicity-Responsive (NFAT5))

Binding Specificity: pSer1197

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NFAT5 antibody is conjugated to Cy5

Application: Western Blotting (WB), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human NFAT5 around the phosphorylation site of Ser1197

Isotype: IgG

Application Details

Application Notes: IF(IHC-P) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for NFAT5

Target: NFAT5 (Nuclear Factor of Activated T-Cells 5, Tonicity-Responsive (NFAT5))

Alternative Name: NFAT5 (NFAT5 Products)

Synonyms: zgc:63502 antibody, wu:fi46f11 antibody, TonEBP antibody, NFAT5 antibody, nfatz antibody, orebp antibody, nf-at5 antibody, nfatl1 antibody, tonebp antibody, DKFZp469P1514 antibody, AI225870 antibody, B130038B15Rik antibody, CAG-8 antibody, CAG80 antibody, NFATL1 antibody, OREBP antibody, mKIAA0827 antibody, NF-AT5 antibody, NFATZ antibody, TONEBP antibody, Nfat antibody, nuclear factor of activated T-cells 5 antibody, nuclear factor of activated T-cells 5, tonicity-responsive a antibody, nuclear factor of activated T-cells 5, tonicity-responsive S homeolog antibody, nuclear factor of activated T-cells 5, tonicity-responsive antibody, nuclear factor of activated T cells 5 antibody, NFAT5 antibody, nfat5a antibody, nfat5.S antibody, nfat5 antibody, Nfat5 antibody

Background:

Synonyms: NFAT5 phospho S1197, p-NFAT5phospho S1197, Glutamine rich protein H65, KIAA0827, NF AT5, NF-AT5, NFAT 5, NFAT L1, NFAT like protein 1, NFAT5, NFAT5_HUMAN, NFATL 1, NFATL1, NFATZ, Nuclear factor of activated T cells 5, Nuclear factor of activated T cells 5 tonicity responsive, Nuclear factor of activated T cells, Nuclear factor of activated T-cells 5, OREBP, Osmotic response element binding protein, T cell transcription factor NFAT 5, T cell transcription factor NFAT5, T-cell transcription factor NFAT5, TonE binding protein, TonE-binding protein , TonEBP, Tonicity responsive enhancer binding protein, Tonicity-responsive enhancer-binding protein.

Background: Members of the NFAT (nuclear factor of activated T cells) family of transcription factors are related to NFkB/Rel proteins and form cooperative complexes with the AP-1 proteins, Fos and Jun, on DNA to regulate cytokine expression in T cells. NFAT proteins are widely expressed and alternatively modified to generate splice variants, and they are localized to both the cytosol (NFATc) and to the nucleus (NFATn). NFAT1, NFAT2, and NFAT4 are predominantly expressed in immune cells, and NFAT2 and NFAT3 are expressed at high levels in cardiac tissues. In addition to activating cytokine gene transcription, NFAT2 is also implicated in cardiac valve development, and NFAT3 is involved in cardiac hypertrophy. NFAT5 is detected in both immune and nonimmune cells and, like other NFAT proteins, contains a highly conserved Rel-like binding domain that mediates NFAT proteins associating with specific consensus sequences on DNA. NFAT proteins are activated by increases in intracellular calcium, which leads to the calmodulin-dependent phosphatase, calcineurin, dephosphorylating NFAT proteins. This activating event induces a conformational change in the protein structure that exposes the nuclear localization signal and facilitates the translocation of NFAT proteins from the cytosol into the nucleus.

Gene ID: 10725

Pathways: RTK Signaling, WNT Signaling