H/K/N-Ras antibody

Antigen: H/K/N-Ras

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse (Murine), Rat (Rattus)

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN142310

Additional Information

Alternative name: HRAS (H-, K- and N-Ras)

Immunogen: A synthetic peptide corresponding to amino acid 31 ?C 43 (EYDPTIEDSYRKQ) from L2 loop of human Ras protein.

Isotype: IgG  (Matching secondary antibodies)

Description: This antibody reacts with a 21kD protein. Each mammalian cell contains three Ras proto-oncogene coding for closely related Ras proteins: H-, K- and N-Ras. Oncogenic mutations in Ras genes are present in ~30% of all human cancers. K-Ras mutations are common in pancreatic, colorectal and non-small-cell lung carcinomas, H-Ras mutations are common in bladder, kidney and thyroid carcinomas, N-Ras mutations are found in melanoma, hematological malignancies and hepatocellular carcinoma. This antibody stain H-, K- and N-Ras proteins. This antibody cross reacts with mouse, rat and cow.

Application Details

Application Notes: This antibody may be diluted to a titer of 1:50-1:100 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation conditions should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining.

Purity: Purified immunoglobulin fraction of rabbit antiserum against Ras containing sodium azide as preservative.

Buffer: PBS with sodium azide

Storage: Store the antibody at 4-8 C. Do not use it beyond the expiration date stated on the label.

Restrictions: For Research Use only