Nibrin (NBN) (AA 1-705) antibody

Details for Product No. ABIN142528
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Antigen
Synonyms NBN, AT-V1, AT-V2, ATV, NBS, NBS1, P95, Nbs1, im:6911679, zgc:194152
Epitope
AA 1-705
(48), (20), (8), (8), (7), (6), (6), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(221), (81), (55), (24), (24), (24)
Host
Rabbit
(178), (47)
Clonality
Polyclonal
Conjugate
Un-conjugated
(7), (6), (5), (4), (4), (4), (4), (4), (4), (4), (4), (2), (2), (2)
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
(183), (76), (46), (40), (34), (27), (23), (21), (12), (2), (2), (1)
Pubmed 5 references available
Quantity 50 μL
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Catalog No. ABIN142528
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Immunogen NBS1 (aa 1-705)
Specificity This antibody is specific for hp95.
Purification Whole antisera.
Alternative Name Nibrin
Background The p95 gene (identical to NBS1 and nibrin) is a member of the hMre11/hRad50 double-strand break complex. This protein complex has been implicated in Nijmegen breakage syndrome, an autosomal recessive disorder marked by increased cancer incidence, cell cycle checkpoint deficits, and ionizing radiation sensitivity, thus revealing a direct molecular link between double-strand break repair and cell cycle checkpoint functions.
Synonyms: MGC87362, P95, FLJ10155, ATV, NBS, ATV2, ATV1, NBS1, AT-V1, AT-V2, AT V1, AT V2
Application Notes By Western blot, this antibody recognizes a band at 95 kDa representing hp95 in TK6 or other human cells. By immunofluorescence, this antibody has been used with methanol-fixed IMR90 primary human fibroblasts. Immunoprecipitation tests have been done with 3-4 X 106 cells. Suggested working dilutions: * Western Blot-1:1,000-1:10,000 (~95 kDa band, representing hp95) Immunofluorescence: 1:200 (used with methanol-fixed IMR90 primary human fibroblasts) Immunoprecipitation: 3 ul for 3 x 10-6 cells or 1-4 ug/mg lysate. *The investigator should determine the optimal working dilution for a specific application. Other applications have not been tested.
Comment

Positive Controls: Hela whole cell extract

Restrictions For Research Use only
Format Liquid
Buffer 50 µl of antisera containing 0.02% sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze
Storage 4 °C
Storage Comment Keep as concentrated solution, aliquot and store at 4°C.
Supplier Images
anti-Nibrin (NBN) (AA 1-705) antibody anti-Nibrin (NBN) (AA 1-705) antibody
General Paull, Rogakou, Yamazaki et al.: "A critical role for histone H2AX in recruitment of repair factors to nuclear foci after DNA damage." in: Current biology : CB, Vol. 10, Issue 15, pp. 886-95, 2000 (PubMed).

Harfst, Cooper, Neubauer et al.: "Normal V(D)J recombination in cells from patients with Nijmegen breakage syndrome." in: Molecular immunology, Vol. 37, Issue 15, pp. 915-29, 2001 (PubMed).

Franchitto, Pichierri: "Bloom's syndrome protein is required for correct relocalization of RAD50/MRE11/NBS1 complex after replication fork arrest." in: The Journal of cell biology, Vol. 157, Issue 1, pp. 19-30, 2002 (PubMed).

Cerosaletti, Concannon: "Nibrin forkhead-associated domain and breast cancer C-terminal domain are both required for nuclear focus formation and phosphorylation." in: The Journal of biological chemistry, Vol. 278, Issue 24, pp. 21944-51, 2003 (PubMed).

Siwicki, Degerman, Chrzanowska et al.: "Telomere maintenance and cell cycle regulation in spontaneously immortalized T-cell lines from Nijmegen breakage syndrome patients." in: Experimental cell research, Vol. 287, Issue 1, pp. 178-89, 2003 (PubMed).

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