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Antibodies
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anti-DYKDDDDK Epitope Tag antibody (HRP)
DYKDDDDK Epitope Tag antibody (HRP)
| Antigen |
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| Clonality |
Polyclonal |
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Host
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Conjugate
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Application
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 Alternatives Enzyme Immunoassay (EIA), Western Blotting (WB)
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| Catalog no. |
ABIN142789 |
| Quantity |
0.1 mg (1 mg/ml) |
| Price |
Product not available in this region.
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| Shipping to |
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Immunogen
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Rabbits were immunized with FLAG peptide conjugated to KLH. After multiple immunizations in Freunds adjuvant, serum was collected and the antibodies were affinity purified using the peptide immobilized on a solid phase. The affinity purified antibodies w
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Description
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HRP Conjugated Rabbit anti-Flag
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Application Notes
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WB: 1:2000-1:10,000, ELISA: 1:5,000-1:10,000. Antibody was anayzed using amino-terminal Met-Flag-BAP, amino-terminal FLAG-BAP and carboxy-terminal FLAG-BAP fusion proteins and Invitrogen Positope R900-40 Not tested in other applications. Optimal dilutions/concentrations should be determined by the researcher
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Concentration
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1 mg/ml
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Buffer
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50 mM HEPES pH7.0, 0.1 M Sodium Chloride with 0.2% BSA, 0.01% thimerosal
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Storage
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4 C
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Research Area
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Tags/Labels
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Restrictions
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For Research Use only
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Alternatives for antigen "DYKDDDDK Epitope Tag", type "Antibodies"
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Hosts
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Rabbit (15), Mouse (6), Sheep (1)
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Reactivities
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Mammalian (2), Various Species (2), Human (1)
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Applications
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Western Blotting (WB) (21), Enzyme Immunoassay (EIA) (15), Immunoprecipitation (IP) (7), Immunofluorescence (IF) (6), Immunohistochemistry (Frozen Sections) (IHC (fro)) (4), Flow Cytometry (FACS) (2), ELISA (1), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (1)
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Conjugates
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Biotin (1), HRP (1)
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