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Catenin beta-1 (CTNB1) antibody

Details for Product No. ABIN143811
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Antigen
Synonyms Bfc, Catnb, Mesc, ctnnb, id:ibd2058, wu:fb73e10, wu:fi81c06, wu:fk25h01, CHBCAT
Reactivity
Chicken
(10), (2), (2)
Host
Mouse
(10), (4)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(1)
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB)
(12), (7), (6), (6), (3), (2), (2), (2), (1), (1)
Pubmed 6 references available
Quantity 0.1 mL
Shipping to United States (Change)
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Catalog No. ABIN143811
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Immunogen Recombinant full length protein (Chicken).
Clone 6F9
Isotype IgG1
Specificity Recognises beta-catenin (94kD) in immunoblotting. Does not cross-react with plakoglobin.
Cross-Reactivity Human, Cow (Bovine), Dog (Canine)
Characteristics Cellular Localization: Cytoplasmic when it is unstabilized (high level of phosphorylation) or bound to CDH1. Translocates to the nucleus when it is stabilized (low level of phosphorylation).
Purification Ascites.
Alternative Name Catenin beta-1
Background The distinct peripheral cytosolic proteins, alpha, beta and gamma-catenin (102, 94 and 86 kD) found in many tissues bind to the conserved cytoplasmic tail domain of the cell-adhesion cadherins. Catenins link E-cadherin to other integral membrane or cytoplasmic proteins and are modulated by Wnt-1 protooncogene. They are good candidates for mediating transduction of cell-cell contact positional signals to the cell interior. Absence of alpha-catenin is found in certain tumor cell lines and reduced levels in certain human carcinomas. Beta-catenin binds directly to the cytoplasmic tail of E-cadherin. It binds to the N-terminus of alpha-catenin and interacts with the protein product of the tumor suppressor gene APC. This interaction involves a 15-aa repeat in the APC. Beta-catenin cell levels seem to be controlled by APC. The central core region of beta-catenin is involved in mediation of cadherin-catenin complex interaction with EGFR.
Synonyms: CTNNB1, P35222, 114550, 1499, 395964, beta Catenin, CHBCAT, CTNNB
Research Area Signaling
Application Notes ICC: Use at an assay dependant dilution. IF: Use at a dilution of 1/1000. WB: Use at a dilution of 1/1000. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Comment

Positive Controls: MDBK cells

Restrictions For Research Use only
Format Liquid
Buffer Ascitic fluid with 0.1% sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles.
Storage 4 °C
Storage Comment Keep as concentrated solution. Store at 4°C short term. For extended storage aliquot and store at -20°C or below.
General Edelman, Crossin: "Cell adhesion molecules: implications for a molecular histology." in: Annual review of biochemistry, Vol. 60, pp. 155-90, 1991 (PubMed).

Takeichi: "Cadherin cell adhesion receptors as a morphogenetic regulator." in: Science (New York, N.Y.), Vol. 251, Issue 5000, pp. 1451-5, 1991 (PubMed).

Nagafuchi, Takeichi: "Cell binding function of E-cadherin is regulated by the cytoplasmic domain." in: The EMBO journal, Vol. 7, Issue 12, pp. 3679-84, 1989 (PubMed).

Reynolds, Daniel, McCrea et al.: "Identification of a new catenin: the tyrosine kinase substrate p120cas associates with E-cadherin complexes." in: Molecular and cellular biology, Vol. 14, Issue 12, pp. 8333-42, 1994 (PubMed).

Tsukita, Itoh, Nagafuchi et al.: "Submembranous junctional plaque proteins include potential tumor suppressor molecules." in: The Journal of cell biology, Vol. 123, Issue 5, pp. 1049-53, 1994 (PubMed).

Johnson, Lewis, Li et al.: "P- and E-cadherin are in separate complexes in cells expressing both cadherins." in: Experimental cell research, Vol. 207, Issue 2, pp. 252-60, 1993 (PubMed).

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