58K Golgi Protein antibody

Details for Product No. ABIN144232
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Synonyms wu:fp37b11, FTCD
Dog (Canine)
(21), (11), (5), (4), (1), (1)
(13), (4), (4)
Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB), Immunoelectron Microscopy (IEM)
(21), (18), (10), (2), (2), (1), (1)
Pubmed 6 references available
Catalog no. ABIN144232
Quantity 0.1 mL
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Immunogen Full length native protein (purified) (Rat liver).
Isotype IgG1
Specificity The product recognizes an epitope located on the microtubule-binding peripheral Golgi membrane 58 kDa protein.
Cross-Reactivity Hamster, Human, Rat (Rattus)
Purification Ascites
Background 58K protein antibodies are excellent for use as markers for the Golgi complex. The 58K protein has been identified as being FTCD, a bifunctional enzyme that channels 1-carbon units from formiminoglutamate, a metabolite of the histidine degradation pathway, to the folate pool.
Application Notes FuncS: Use at an assay dependent dilution. ICC: 1/50. Recommended protocol available. IF: 1/250. IM: Use at an assay dependent dilution. WB: 1/5000. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Restrictions For Research Use only
Format Liquid
Buffer Ascitic fluid with 15mM sodium azide
Preservative Sodium azide
Storage 4 °C
General Ktistakis, Roth, Bloom: "PtK1 cells contain a nondiffusible, dominant factor that makes the Golgi apparatus resistant to brefeldin A." in: The Journal of cell biology, Vol. 113, Issue 5, pp. 1009-23, 1991 (PubMed).

Donaldson, Lippincott-Schwartz, Bloom et al.: "Dissociation of a 110-kD peripheral membrane protein from the Golgi apparatus is an early event in brefeldin A action." in: The Journal of cell biology, Vol. 111, Issue 6 Pt 1, pp. 2295-306, 1991 (PubMed).

Bloom, Brashear: "A novel 58-kDa protein associates with the Golgi apparatus and microtubules." in: The Journal of biological chemistry, Vol. 264, Issue 27, pp. 16083-92, 1989 (PubMed).

Missiaen, Van Acker, Parys et al.: "Baseline cytosolic Ca2+ oscillations derived from a non-endoplasmic reticulum Ca2+ store." in: The Journal of biological chemistry, Vol. 276, Issue 42, pp. 39161-70, 2001 (PubMed).

Cocucci, Racchetti, Podini et al.: "Enlargeosome, an exocytic vesicle resistant to nonionic detergents, undergoes endocytosis via a nonacidic route." in: Molecular biology of the cell, Vol. 15, Issue 12, pp. 5356-68, 2004 (PubMed).

Drapkin, von Horsten, Lin et al.: "Human epididymis protein 4 (HE4) is a secreted glycoprotein that is overexpressed by serous and endometrioid ovarian carcinomas." in: Cancer research, Vol. 65, Issue 6, pp. 2162-9, 2005 (PubMed).

Hosts (13), (4), (4)
Reactivities (21), (11), (5), (4), (1), (1)
Applications (21), (18), (10), (2), (2), (1), (1)
Epitopes (4), (4), (3), (2)
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