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Details for Product No. ABIN144232

58K Golgi Protein antibody

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Antigen
Synonyms wu:fp37b11, FTCD
Reactivity
»Alternatives Dog (Canine)
Host
»Alternatives Mouse
Clonality Monoclonal
Application
»Alternatives Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB), Immunoelectron Microscopy (IEM)
Pubmed 6 references available
Catalog no. ABIN144232
Quantity 0.1 mL
Price
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Immunogen Full length native protein (purified) (Rat liver).
Isotype IgG1
Specificity The product recognizes an epitope located on the microtubule-binding peripheral Golgi membrane 58 kDa protein.
Cross-Reactivity Hamster, Human, Rat (Rattus)
Purification Ascites
Background 58K protein antibodies are excellent for use as markers for the Golgi complex. The 58K protein has been identified as being FTCD, a bifunctional enzyme that channels 1-carbon units from formiminoglutamate, a metabolite of the histidine degradation pathway, to the folate pool.
Application Notes FuncS: Use at an assay dependent dilution. ICC: 1/50. Recommended protocol available. IF: 1/250. IM: Use at an assay dependent dilution. WB: 1/5000. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Restrictions For Research Use only
Format Liquid
Buffer Ascitic fluid with 15mM sodium azide
Preservative Sodium azide
Storage 4 °C
Ktistakis, Roth, Bloom: "PtK1 cells contain a nondiffusible, dominant factor that makes the Golgi apparatus resistant to brefeldin A." in: The Journal of cell biology, Vol. 113, Issue 5, pp. 1009-23, 1991 (PubMed).

Donaldson, Lippincott-Schwartz, Bloom et al.: "Dissociation of a 110-kD peripheral membrane protein from the Golgi apparatus is an early event in brefeldin A action." in: The Journal of cell biology, Vol. 111, Issue 6 Pt 1, pp. 2295-306, 1991 (PubMed).

Bloom, Brashear: "A novel 58-kDa protein associates with the Golgi apparatus and microtubules." in: The Journal of biological chemistry, Vol. 264, Issue 27, pp. 16083-92, 1989 (PubMed).

Missiaen, Van Acker, Parys et al.: "Baseline cytosolic Ca2+ oscillations derived from a non-endoplasmic reticulum Ca2+ store." in: The Journal of biological chemistry, Vol. 276, Issue 42, pp. 39161-70, 2001 (PubMed).

Cocucci, Racchetti, Podini et al.: "Enlargeosome, an exocytic vesicle resistant to nonionic detergents, undergoes endocytosis via a nonacidic route." in: Molecular biology of the cell, Vol. 15, Issue 12, pp. 5356-68, 2004 (PubMed).

Drapkin, von Horsten, Lin et al.: "Human epididymis protein 4 (HE4) is a secreted glycoprotein that is overexpressed by serous and endometrioid ovarian carcinomas." in: Cancer research, Vol. 65, Issue 6, pp. 2162-9, 2005 (PubMed).

Alternatives for antigen "58K Golgi Protein", type "Antibodies"
Hosts (14), (7), (5)
Reactivities (26), (14), (6), (4), (2), (2)
Applications (26), (20), (12), (3), (3), (2), (1), (1)
Epitopes (4), (4), (3), (3), (1)
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