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|+1 404 474 4654|
|+1 888 205 9894 (TF)|
58K Golgi Protein antibody
|Synonyms||LCHC1, wu:fp37b11, MGC64458, Ftcd, MGC63647, FTCD, ftcd, MGC89380|
Alternatives Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB), Immunoelectron Microscopy (IEM)
|6 references available|
|Price||Product not available in this region.|
|Immunogen||Full length native protein (purified) (Rat liver).|
|Cross-Reactivity||Dog (Canine), Hamster, Human, Rat (Rattus)|
|Description||58K protein antibodies are excellent for use as markers for the Golgi complex. The 58K protein has been identified as being FTCD, a bifunctional enzyme that channels 1-carbon units from formiminoglutamate, a metabolite of the histidine degradation pathway, to the folate pool.|
|Specificity||The product recognizes an epitope located on the microtubule-binding peripheral Golgi membrane 58 kDa protein.|
|Application Notes||FuncS: Use at an assay dependent dilution. ICC: 1/50. Recommended protocol available. IF: 1/250. IM: Use at an assay dependent dilution. WB: 1/5000. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.|
|Buffer||Ascitic fluid with 15mM sodium azide|
|Storage||Store at 4 C for up to one month. For extended storage, add glycerol to 50% and store at -20 C. Repeated freeze-thawing is not recommended. Specific References:|
|Restrictions||For Research Use only|
Ktistakis, Roth, Bloom: "PtK1 cells contain a nondiffusible, dominant factor that makes the Golgi apparatus resistant to brefeldin A." in: The Journal of cell biology, Vol. 113, Issue 5, pp. 1009-23, 1991 (PubMed).
Donaldson, Lippincott-Schwartz, Bloom et al.: "Dissociation of a 110-kD peripheral membrane protein from the Golgi apparatus is an early event in brefeldin A action." in: The Journal of cell biology, Vol. 111, Issue 6 Pt 1, pp. 2295-306, 1991 (PubMed).
Bloom, Brashear: "A novel 58-kDa protein associates with the Golgi apparatus and microtubules." in: The Journal of biological chemistry, Vol. 264, Issue 27, pp. 16083-92, 1989 (PubMed).
Missiaen, Van Acker, Parys et al.: "Baseline cytosolic Ca2+ oscillations derived from a non-endoplasmic reticulum Ca2+ store." in: The Journal of biological chemistry, Vol. 276, Issue 42, pp. 39161-70, 2001 (PubMed).
Cocucci, Racchetti, Podini et al.: "Enlargeosome, an exocytic vesicle resistant to nonionic detergents, undergoes endocytosis via a nonacidic route." in: Molecular biology of the cell, Vol. 15, Issue 12, pp. 5356-68, 2004 (PubMed).
Drapkin, von Horsten, Lin et al.: "Human epididymis protein 4 (HE4) is a secreted glycoprotein that is overexpressed by serous and endometrioid ovarian carcinomas." in: Cancer research, Vol. 65, Issue 6, pp. 2162-9, 2005 (PubMed).
|Hosts||Goat (9), Rabbit (5)|
|Reactivities||Human (14), Mouse (Murine) (14), Pig (Porcine) (6), Rat (Rattus) (4), Chicken (2), Zebrafish (2), Dog (Canine) (1), Xenopus laevis (1)|
|Applications||Western Blotting (WB) (14), ELISA (10), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (6), Flow Cytometry (FACS) (1), Immunohistochemistry (IHC) (1)|