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58K Golgi Protein antibody

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Antigen
Synonyms wu:fp37b11, FTCD
Reactivity
Rat (Rattus)
(15), (7), (5), (3)
Host
Mouse
(9), (4), (2)
Clonality (Clone)
Monoclonal ()
Application
Immunoelectron Microscopy (IEM), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(13), (11), (6), (4), (3), (2)
Pubmed 6 references available
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Quantity 0.1 mL
Shipping to United States ( )
Immunogen Full length native protein (purified) (Rat liver).
Clone 58K-9
Isotype IgG1
Specificity The product recognizes an epitope located on the microtubule-binding peripheral Golgi membrane 58 kDa protein.
Cross-Reactivity Human, Cow (Bovine), Dog (Canine), Hamster, Pig (Porcine)
Characteristics Cellular Localization: Cytoplasmic and Golgi Apparatu
Purification Ascites.
Background 58K protein antibodies are excellent for use as markers for the Golgi complex. The 58K protein has been identified as being FTCD, a bifunctional enzyme that channels 1-carbon units from formiminoglutamate, a metabolite of the histidine degradation pathway, to the folate pool.
Synonyms: 89833, 58K Golgi protein, Ftcd
Application Notes FuncS: Use at an assay dependent dilution. ICC: 1/50. Recommended protocol available. IF: 1/250. IM: Use at an assay dependent dilution. WB: 1/5000. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Comment

Positive Controls: For indirect immunofluorescence: cultured Chinese hamster ovary (CHO) cells For immunoblotting (colorimetric): whole rat liver extractAntigen MW: 58 kDa

Restrictions For Research Use only
Format Liquid
Buffer Ascitic fluid with 15mM sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles.
Storage 4 °C
Storage Comment Keep as concentrated solution. Store at 4°C short term. For extended storage aliquot and store at -20°C or below.
Supplier Images
Immunofluorescence (IF) image for anti-58K Golgi Protein antibody (ABIN144232) anti-58K Golgi Protein antibody
Immunofluorescence (IF) image for anti-58K Golgi Protein antibody (ABIN144232) anti-58K Golgi Protein antibody (Image 2)
Immunohistochemistry (IHC) image for anti-58K Golgi Protein antibody (ABIN144232) anti-58K Golgi Protein antibody (Image 3)
Immunohistochemistry (IHC) image for anti-58K Golgi Protein antibody (ABIN144232) anti-58K Golgi Protein antibody (Image 4)
Immunofluorescence (IF) image for anti-58K Golgi Protein antibody (ABIN144232) anti-58K Golgi Protein antibody (Image 5)
Background publications Drapkin, von Horsten, Lin et al.: "Human epididymis protein 4 (HE4) is a secreted glycoprotein that is overexpressed by serous and endometrioid ovarian carcinomas." in: Cancer research, Vol. 65, Issue 6, pp. 2162-9, 2005 (PubMed).

Cocucci, Racchetti, Podini et al.: "Enlargeosome, an exocytic vesicle resistant to nonionic detergents, undergoes endocytosis via a nonacidic route." in: Molecular biology of the cell, Vol. 15, Issue 12, pp. 5356-68, 2004 (PubMed).

Missiaen, Van Acker, Parys et al.: "Baseline cytosolic Ca2+ oscillations derived from a non-endoplasmic reticulum Ca2+ store." in: The Journal of biological chemistry, Vol. 276, Issue 42, pp. 39161-70, 2001 (PubMed).

Bloom, Brashear: "A novel 58-kDa protein associates with the Golgi apparatus and microtubules." in: The Journal of biological chemistry, Vol. 264, Issue 27, pp. 16083-92, 1989 (PubMed).

Donaldson, Lippincott-Schwartz, Bloom et al.: "Dissociation of a 110-kD peripheral membrane protein from the Golgi apparatus is an early event in brefeldin A action." in: The Journal of cell biology, Vol. 111, Issue 6 Pt 1, pp. 2295-306, 1991 (PubMed).

Ktistakis, Roth, Bloom: "PtK1 cells contain a nondiffusible, dominant factor that makes the Golgi apparatus resistant to brefeldin A." in: The Journal of cell biology, Vol. 113, Issue 5, pp. 1009-23, 1991 (PubMed).

Catalog No. ABIN144232
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