58K Golgi Protein antibody

Details for Product No. ABIN144232
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Antigen
Synonyms wu:fp37b11, FTCD
Reactivity
Rat (Rattus)
(19), (9), (5), (5), (1), (1)
Host
Mouse
(11), (4), (4)
Clonality (Clone)
Monoclonal ()
Application
Immunoelectron Microscopy (IEM), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(19), (10), (9), (6), (6), (3), (2), (2), (1)
Pubmed 6 references available
Quantity 0.1 mL
Shipping to United States (Change)
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Catalog No. ABIN144232
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Immunogen Full length native protein (purified) (Rat liver).
Clone 58K-9
Isotype IgG1
Specificity The product recognizes an epitope located on the microtubule-binding peripheral Golgi membrane 58 kDa protein.
Cross-Reactivity Human, Cow (Bovine), Dog (Canine), Hamster, Pig (Porcine)
Characteristics Cellular Localization: Cytoplasmic and Golgi Apparatu
Purification Ascites.
Background 58K protein antibodies are excellent for use as markers for the Golgi complex. The 58K protein has been identified as being FTCD, a bifunctional enzyme that channels 1-carbon units from formiminoglutamate, a metabolite of the histidine degradation pathway, to the folate pool.
Synonyms: 89833, 58K Golgi protein, Ftcd
Application Notes FuncS: Use at an assay dependent dilution. ICC: 1/50. Recommended protocol available. IF: 1/250. IM: Use at an assay dependent dilution. WB: 1/5000. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Comment

Positive Controls: For indirect immunofluorescence: cultured Chinese hamster ovary (CHO) cells For immunoblotting (colorimetric): whole rat liver extractAntigen MW: 58 kDa

Restrictions For Research Use only
Format Liquid
Buffer Ascitic fluid with 15mM sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles.
Storage 4 °C
Storage Comment Keep as concentrated solution. Store at 4°C short term. For extended storage aliquot and store at -20°C or below.
General Ktistakis, Roth, Bloom: "PtK1 cells contain a nondiffusible, dominant factor that makes the Golgi apparatus resistant to brefeldin A." in: The Journal of cell biology, Vol. 113, Issue 5, pp. 1009-23, 1991 (PubMed).

Donaldson, Lippincott-Schwartz, Bloom et al.: "Dissociation of a 110-kD peripheral membrane protein from the Golgi apparatus is an early event in brefeldin A action." in: The Journal of cell biology, Vol. 111, Issue 6 Pt 1, pp. 2295-306, 1991 (PubMed).

Bloom, Brashear: "A novel 58-kDa protein associates with the Golgi apparatus and microtubules." in: The Journal of biological chemistry, Vol. 264, Issue 27, pp. 16083-92, 1989 (PubMed).

Missiaen, Van Acker, Parys et al.: "Baseline cytosolic Ca2+ oscillations derived from a non-endoplasmic reticulum Ca2+ store." in: The Journal of biological chemistry, Vol. 276, Issue 42, pp. 39161-70, 2001 (PubMed).

Cocucci, Racchetti, Podini et al.: "Enlargeosome, an exocytic vesicle resistant to nonionic detergents, undergoes endocytosis via a nonacidic route." in: Molecular biology of the cell, Vol. 15, Issue 12, pp. 5356-68, 2004 (PubMed).

Drapkin, von Horsten, Lin et al.: "Human epididymis protein 4 (HE4) is a secreted glycoprotein that is overexpressed by serous and endometrioid ovarian carcinomas." in: Cancer research, Vol. 65, Issue 6, pp. 2162-9, 2005 (PubMed).

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