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HMB45 Melanoma Marker antibody

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Clonality (Clone)
Monoclonal ()
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC)
(4), (2), (1), (1)
Pubmed 1 reference available
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Quantity 0.25 mL
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Immunogen Extract of pigmented melanoma metastases from lymph nodes.
Clone HMB45
Isotype IgG1
Fragment Light chain,Chain kappa
Specificity This antibody reacts with a neuraminidase sensitive oligosaccharide side chain of a glycoconjugate present in immature melanosomes. It reacts with junctional and blue nevus cells. Non-melanocytic cells are negative.
Characteristics Cellular Localization: Cytoplasmic
Purification Tissue culture supernatant.
Background Synonyms: CMM1, DNS, FAMMM, MLM
Application Notes IHC: This antibody may be diluted to a titer of 1:10-1:50 in an ABC method. Staining Protocol: We suggest an incubation period of 30-60 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation conditions and antibody dilutions should be determined by the user. Enzymatic antigen retrieval may increase staining intensity when using formalin fixed paraffin embedded tissue.
Restrictions For Research Use only
Format Liquid
Concentration 0.025 mg/mL
Buffer Tissue culture supernatant with PBS, 1% BSA and 0.05% sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Keep as concentrated solution, aliquot and store at 4°C.
Supplier Images
 image for anti-HMB45 Melanoma Marker antibody (ABIN144595) anti-HMB45 Melanoma Marker antibody
Background publications Gown, Vogel, Hoak et al.: "Monoclonal antibodies specific for melanocytic tumors distinguish subpopulations of melanocytes." in: The American journal of pathology, Vol. 123, Issue 2, pp. 195-203, 1986 (PubMed).

Catalog No. ABIN144595
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