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Details for Product No. ABIN144595

HMB45 Melanoma Marker antibody

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»Alternatives Human
»Alternatives Mouse
Clonality Monoclonal
»Alternatives Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC)
Pubmed 1 reference available
Catalog no. ABIN144595
Quantity 0.25 mL
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Immunogen Extract of pigmented melanoma allmetastases from lymph nodes.
Isotype IgG1
Specificity This antibody reacts with a neuraminidase sensitive oligosaccharide side chain of a glycoconjugate present in immature melanosomes. It reacts with junctional and blue nevus cells. Non-melanocytic cells are negative.
Purification Tissue culture supernatant
Background Mouse monoclonal [HMB45] to Melanoma
Application Notes IHC: This antibody may be diluted to a titer of 1:10-1:50 in an ABC method. Staining Protocol: We suggest an incubation period of 30-60 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation conditions and antibody dilutions should be determined by the user. Enzymatic antigen retrieval may increase staining intensity when using formalin fixed paraffin embedded tissue.
Restrictions For Research Use only
Format Liquid
Concentration 0.025 mg/ml
Buffer Tissue culture supernatant with sodium azide
Preservative Sodium azide
Storage 4 °C
Gown, Vogel, Hoak et al.: "Monoclonal antibodies specific for melanocytic tumors distinguish subpopulations of melanocytes." in: The American journal of pathology, Vol. 123, Issue 2, pp. 195-203, 1986 (PubMed).

Alternatives for antigen "HMB45 Melanoma Marker", type "Antibodies"
Hosts (6)
Reactivities (6)
Applications (6), (2), (1), (1)