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Serotonin Receptor 5A (HTR5A) antibody

Details for Product No. ABIN144722
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Synonyms HTR5A, si:dkey-265a7.5, Htr5, 5-HT5A, 5HT5A, MR22
(56), (5), (4)
(57), (6)
Clonality (Clone)
Monoclonal ()
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(42), (32), (20), (10), (3), (3), (3), (1)
Pubmed 3 references available
Quantity 0.5 mL
Shipping to United States (Change)
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Catalog No. ABIN144722
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Immunogen 5-hydroxytryptamine (3-(2-aminoethyl)-5-hydroxyindole (serotonin)- conjugated to BSA
Clone 5HT-H209
Isotype IgG1
Fragment Light chain,Chain kappa
Characteristics Cellular Localization: Cytoplasmic
Purification Unpurified. Tissue culture supernatant
Background This Product may be a useful tool for characterization of carcinoids.In a study of 48 cases of adenomas and carcinoid tumours of the middle ear, the antibody labelled tumour cells in 12 cases (25%). In 1 case of carcinoid tumour of the middle ear, the antibody labelled tumour cells. In a study of 43 cases of bronchial carcinoid tumours, the antibody labelled tumour cells in 13 cases (30%). Cells labelled by the antibody display a cytoplasmic staining pattern.
Synonyms: 5HT5A, Serotonin, 5HT, 3361, MGC138226, P47898, 601305, 5-HT5A, HTR5A, 5 HT5A
Application Notes Immunohistochemistry (Paraffin/Frozen) 1:20 -1:40, When using formalin-fixed, paraffin-embedded tissues, a high temperature antigenic unmasking technique is strongly recommended (For formalin-fixed paraffin-embedded (FFPE) tissue sections require boiling the tissue in 10 mM citrate, pH 6.0 buffer for 10 minute, followed by cooling at RT for 20 minutes), OR FFPE tissue sections may be treated with proteases before addition of primary antibody. Can also be use on acetone-fixed cryostat sections or for fixed cell smears at a dilution of 1:20-1:40. The optimum dilution should be determined by the individual lab.

Positive Controls: Appendix

Restrictions For Research Use only
Format Liquid
Buffer Tissue culture supernatant containing 0.1% sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze
Storage 4 °C
Storage Comment Keep as concentrated solution, aliquot and store at 4°C.
General Granberg, Wilander, Oberg et al.: "Prognostic markers in patients with typical bronchial carcinoid tumors." in: The Journal of clinical endocrinology and metabolism, Vol. 85, Issue 9, pp. 3425-30, 2000 (PubMed).

Menezes, Wakely: "Aspiration cytopathology of middle-ear neuroendocrine carcinoma." in: Diagnostic cytopathology, Vol. 25, Issue 3, pp. 168-71, 2001 (PubMed).

Torske, Thompson: "Adenoma versus carcinoid tumor of the middle ear: a study of 48 cases and review of the literature." in: Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc, Vol. 15, Issue 5, pp. 543-55, 2002 (PubMed).

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