EHMT2 antibody (C-Term)

Catalog No. ABIN1449182

Product Details anti-EHMT2 Antibody

Target: EHMT2 (Ankyrin Repeat-Containing Protein (EHMT2))

Binding Specificity: AA 621-1000, C-Term

Reactivity: Human, Mouse

Host: Hamster

Clonality: Monoclonal

Conjugate: This EHMT2 antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: This antibody reacts with both the 165 kDa G9a-L isoform and the 140 kDa G9a-S isoform.

Cross-Reactivity (Details): Species reactivity (tested):Human, Mouse

Purification: Protein A agarose

Immunogen: GST-mouse G9a C-terminal protein (a.a. 621-1000)

Clone: 01-01-14

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS containing 50 % glycerol, pH 7.2. Contains no preservatives.

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for EHMT2

Target: EHMT2 (Ankyrin Repeat-Containing Protein (EHMT2))

Alternative Name: BAT8 (EHMT2 Products)

Background: G9a (BAT8, NG36) is a major histone methyltransferase that acts by adding a methyl group to the Lys9 amino acid on histone H3 proteins. Methylation of Lys9 on histone H3 is important for the regulation of the expression of crucial genes during embryogenesis. Methylation of Lys9 may also play a role in tumorigenesis, since deregulation of histone H3 Lys9 methylation in G9a-deficient cells induces the expression of Mage-2a, a tumor-specific protein whose function is unknown. The 165 kDa G9a protein has an unusual structure, containing 24 contiguous glutamate residues in the N-terminus and 6 contiguous copies of a 33-amino acid ankyrin repeat in the C-terminus. The presence of the ankyrin repeats suggest that G9a may be involved in intracellular protein-protein interactions. G9a is expressed in monocytic, macrophage, hepatocyte, T-cell, B-cell, epithelial, and promyelocytic cell lines.Synonyms: C6orf30, EHMT2, Euchromatic histone-lysine N-methyltransferase 2, H3 lysine-9 specific 3, H3-K9-HMTase 3, HLA-B-associated transcript 8, Histone H3-K9 methyltransferase 3, Histone-lysine N-methyltransferase, KMT1C, Lysine N-methyltransferase 1C, NG36, Protein G9A

Gene ID: 110147

NCBI Accession: NP_665829

UniProt: Q9Z148