MFGE8 antibody (Milk Fat Globule-EGF Factor 8 Protein)

Details for Product anti-MFGE8 Antibody No. ABIN1449223, Supplier: Log in to see
Antigen
  • ba46
  • edil1
  • oacgd3s
  • MGC75581
  • hst19888
  • MGC145109
  • MFGE8
  • mfge8
  • zgc:154157
  • MGC99228
  • BA46
  • EDIL1
  • HMFG
  • HsT19888
  • MFG-E8
  • MFGM
  • OAcGD3S
  • SED1
  • SPAG10
  • hP47
  • AA408458
  • AI325141
  • Mfgm
  • P47
  • lactadherin
  • AGS
  • MFGME8
  • MFGMP-E8
  • milk fat globule-EGF factor 8 protein
  • milk fat globule-EGF factor 8 protein b
  • mfge8
  • MFGE8
  • mfge8b
  • Mfge8
Reactivity
Mouse (Murine)
143
15
4
3
Host
Hamster
79
72
3
2
1
Clonality (Clone)
Monoclonal ()
Conjugate
This MFGE8 antibody is un-conjugated
10
8
8
4
4
4
3
3
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
91
64
53
40
22
18
18
13
6
5
4
2
2
2
1
Options
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Immunogen Recombinant mouse MFG-E8
Clone 18A2-G10
Isotype IgG
Specificity This antibody reacts with MFG-E8 short and long forms.
Cross-Reactivity (Details) Species reactivity (tested):Mouse.
Purification Protein A agarose
Alternative Name Lactadherin (MFGE8 Antibody Abstract)
Background Apoptotic cells are rapidly removed by phagocytosis to clear the living cells and tissues of intracellular materials being released from dying cells. Phagocytes recognize apoptotic cells through milk fat globule-EGF-factor 8 (MFG-E8). MFG-E8 is a secreted glycoprotein from thioglycollate-elicited macrophages and bind to phosphatidylserine exposed on the cell surface of apoptotic cells, then bind strongly to cells expressing avß3 integrin via its RGD (arginine-glycine-aspartate) motif. Thus, MFG-E8 is an important protein to mediate the engulfment of apoptotic cells by activated macrophages.Synonyms: Breast epithelial antigen BA46, HMFG, MFGE8, MFGM, Milk fat globule-EGF factor 8
Gene ID 4240
NCBI Accession NP_001108086
UniProt Q08431
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Concentration 1.0 mg/mL
Buffer PBS containing 50 % Glycerol, pH 7.2, None
Preservative Without preservative
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Upon receipt, store (in aliquots) at -20 °C.
Supplier Images
Western Blotting (WB) image for anti-MFGE8 antibody (Milk Fat Globule-EGF Factor 8 Protein) (ABIN1449223) anti-Milk Fat Globule-EGF Factor 8 Protein (MFGE8) antibody
Background publications Kranich, Krautler, Heinen, Polymenidou, Bridel, Schildknecht, Huber, Kosco-Vilbois, Zinkernagel, Miele, Aguzzi: "Follicular dendritic cells control engulfment of apoptotic bodies by secreting Mfge8." in: The Journal of experimental medicine, Vol. 205, Issue 6, pp. 1293-302, 2008 (PubMed).

Hanayama, Nagata: "Impaired involution of mammary glands in the absence of milk fat globule EGF factor 8." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 102, Issue 46, pp. 16886-91, 2005 (PubMed).

Hanayama, Tanaka, Miyasaka, Aozasa, Koike, Uchiyama, Nagata: "Autoimmune disease and impaired uptake of apoptotic cells in MFG-E8-deficient mice." in: Science (New York, N.Y.), Vol. 304, Issue 5674, pp. 1147-50, 2004 (PubMed).

Miyasaka, Hanayama, Tanaka, Nagata: "Expression of milk fat globule epidermal growth factor 8 in immature dendritic cells for engulfment of apoptotic cells." in: European journal of immunology, Vol. 34, Issue 5, pp. 1414-22, 2004 (PubMed).

Hanayama, Tanaka, Miwa, Nagata: "Expression of developmental endothelial locus-1 in a subset of macrophages for engulfment of apoptotic cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 172, Issue 6, pp. 3876-82, 2004 (PubMed).