PD-L1 antibody (Extracellular Domain)

Catalog No. ABIN1449244

Product Details anti-PD-L1 Antibody

Target: PD-L1 (CD274 (PD-L1))

Binding Specificity: Extracellular Domain

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This PD-L1 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: This antibody reacts with human CD274 antigen.

Purification: Protein A agarose

Immunogen: Recombinant human PD-L1 extracellular domain

Clone: 27A2

Isotype: IgG2b

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS containing 50 % glycerol, pH 7.2. No preservative is contained.

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Upon receipt, store (in aliqouts) at -20 °C.

References for anti-PD-L1 antibody (ABIN1449244)

Gadiot, Hooijkaas, Kaiser, van Tinteren, van Boven, Blank: "Overall survival and PD-L1 expression in metastasized malignant melanoma." in: Cancer, Vol. 117, Issue 10, pp. 2192-201, (2011) (PubMed).

Hino, Kabashima, Kato, Yagi, Nakamura, Honjo, Okazaki, Tokura: "Tumor cell expression of programmed cell death-1 ligand 1 is a prognostic factor for malignant melanoma." in: Cancer, Vol. 116, Issue 7, pp. 1757-66, (2010) (PubMed).

Schuchmann, Meyer, Distler, von Stebut, Kuball, Schnürer, Wölfel, Theobald, Konur, Gregor, Schreiner, Huber, Galle, Otto, Herr: "The programmed death (PD)-1/PD-ligand 1 pathway regulates graft-versus-host-reactive CD8 T cells after liver transplantation." in: American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons, Vol. 8, Issue 11, pp. 2434-44, (2008) (PubMed).

Fancke, Suter, Hochrein, OKeeffe: "M-CSF: a novel plasmacytoid and conventional dendritic cell poietin." in: Blood, Vol. 111, Issue 1, pp. 150-9, (2008) (PubMed).

Hamanishi, Mandai, Iwasaki, Okazaki, Tanaka, Yamaguchi, Higuchi, Yagi, Takakura, Minato, Honjo, Fujii: "Programmed cell death 1 ligand 1 and tumor-infiltrating CD8+ T lymphocytes are prognostic factors of human ovarian cancer." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 104, Issue 9, pp. 3360-5, (2007) (PubMed).

Vega, Jazirehi, Huerta-Yepez, Bonavida et al.: "Rituximab-induced inhibition of YY1 and Bcl-xL expression in Ramos non-Hodgkin's lymphoma cell line via inhibition of NF-kappa B activity: role of YY1 and Bcl-xL in Fas resistance and ..." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 175, Issue 4, pp. 2174-83, (2005) (PubMed).

Target Details for PD-L1

Target: PD-L1 (CD274 (PD-L1))

Alternative Name: CD274 / PDL1 (PD-L1 Products)

Synonyms: B7-H antibody, B7H1 antibody, PD-L1 antibody, PDCD1L1 antibody, PDCD1LG1 antibody, PDL1 antibody, A530045L16Rik antibody, B7h1 antibody, Pdcd1l1 antibody, Pdcd1lg1 antibody, Pdl1 antibody, RGD1566211 antibody, CD274 molecule antibody, CD274 antigen antibody, programmed cell death 1 ligand 2 antibody, CD274 antibody, Cd274 antibody, PDCD1LG2 antibody

Background: Programmed death ligand 1 (PD-L1, also known as CD274/B7-H1), a member of B7 family was identified by searching for mo lecules that share homology with the immunogloblin V and C domains of B7-1 and B7-2 among the human cDNA expressed sequence tags in the National Center for Biotechnology Information database. PD-L1 is a ligand for programmed death 1 (PD-1) which belongs to the CD28/CTLA4 subfamily. Although in vitro study indicated that the cross-linking of PD-1 by PD-L1 leads to down-regulation of T-cell responses, some studies have shown that T cells stimulated with low levels of anti-CD3 and immobilized PD-L1-Ig were activated, proliferation and production of IFN- γ GM-CSF and IL-10 from the T cells were enhanced. The role of PD-L1 is now debatable.Synonyms: B7 homolog 1, B7H1, PD-L1, PDCD1 ligand 1, PDCD1L1, PDCD1LG1, Programmed cell death 1 ligand 1, Programmed death ligand 1

Gene ID: 29126

NCBI Accession: NP_054862

UniProt: Q9NZQ7

Pathways: Cancer Immune Checkpoints