Epidermal Growth Factor (EGF) (AA 648-1186) antibody

Details for Product No. ABIN1449300
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Antigen
Synonyms HOMG4, URG, AI790464, CEGF
Epitope
AA 648-1186
(8), (7), (7), (6), (5), (4), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(213), (80), (58), (6), (6), (4), (4), (4), (3)
Host
Mouse
(196), (111), (14), (5), (4), (1)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(33), (15), (13), (6), (6), (6), (6), (6), (6), (6), (6), (5), (2), (2)
Application
Flow Cytometry (FACS), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
(209), (173), (60), (54), (49), (35), (29), (27), (26), (14), (13), (11), (9), (7), (7), (5), (2), (2), (2), (1)
Pubmed 9 references available
Quantity 0.1 mg
Shipping to United States (Change)
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Catalog No. ABIN1449300
335.50 $
Plus shipping costs $45.00

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Immunogen Recombinant human EGF-R cytoplasmic region (648-1186 aa)
Clone 6F1
Isotype IgG2b
Specificity This antibody reacts with Human EGF-R. It does not recognize human c-erbB2.
Purification Supernatant
Alternative Name EGF
Background The epidermal growth factor receptor (EGFR, ErbB-1) is a 170 kDa transmembrane glycoprotein that possesses intrinsic tyrosine kinase activity. There are several EGF family ligands including epidermal growth factor (EGF), amphiregulin (AR), heparin-binding epidermal growth factor (HB-EGF), epiregulin, betacellulin, transforming growth factor-a (TGF-a) and epigen, which can bind and activate the EGFR. Ligand binding facilitates dimerization of the EGFR, which activates downstream pathways known to be involved in cell growth, proliferation, differentiation and migration. Other members of the receptor tyrosine kinase family, which include EGFR, ErbB-2/HER-2, ErbB-3/HER-3 and ErbB-4/HER-4, are also known to play important roles in regulating a wide variety of cellular functions. Overexpression by gene amplification or mutation of these receptors correlates with the development and progression of several human cancers, as well as poor prognosis. Consequently, these receptors have been identified not only as a possible prognostic markers but also primarily a rational molecular target for an anticancer agents.Synonyms: Epidermal growth factor, HOMG4, Pro-epidermal growth factor, URG, Urogastrone
Gene ID 1950
NCBI Accession NP_001954
UniProt P01133
Research Area Cancer, Growth Factors
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Reconstitution Prepare a stock solution by dissolving the lyophilized antibody in 1 mL of distilled water. After reconstitution, the protein concentration should be 1.1 mg/mL and the IgG concentration should be 100 μg/mL.
Buffer PBS ( pH 7.2), 0.09 % NaN3, 1 % Sucrose
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Prior to reconstitution store at 2-8 °C. Following reconstitution store undiluted in aliquots at -20 °C.
Supplier Images
anti-Epidermal Growth Factor (EGF) (AA 648-1186) antibody anti-Epidermal Growth Factor (EGF) (AA 648-1186) antibody
Background publications Krueger, Daaka, Pitcher et al.: "The role of sequestration in G protein-coupled receptor resensitization. Regulation of beta2-adrenergic receptor dephosphorylation by vesicular acidification." in: The Journal of biological chemistry, Vol. 272, Issue 1, pp. 5-8, 1997 (PubMed).

Lazarovici, Oshima, Shavit et al.: "Down-regulation of epidermal growth factor receptors by nerve growth factor in PC12 cells is p140(trk)-, Ras-, and Src-dependent." in: The Journal of biological chemistry, Vol. 272, Issue 17, pp. 11026-34, 1997 (PubMed).

Shibutani, Lazarovici, Johnson et al.: "Transcriptional down-regulation of epidermal growth factor receptors by nerve growth factor treatment of PC12 cells." in: The Journal of biological chemistry, Vol. 273, Issue 12, pp. 6878-84, 1998 (PubMed).

Hashimoto, Katayama, Kiyokawa et al.: "Phosphorylation of CrkII adaptor protein at tyrosine 221 by epidermal growth factor receptor." in: The Journal of biological chemistry, Vol. 273, Issue 27, pp. 17186-91, 1998 (PubMed).

Liu, Gong, Guo et al.: "The Wilms' tumor gene product WT1 mediates the down-regulation of the rat epidermal growth factor receptor by nerve growth factor in PC12 cells." in: The Journal of biological chemistry, Vol. 276, Issue 7, pp. 5068-73, 2001 (PubMed).

Higuchi, Watanabe, Yamamoto: "Protein kinase A regulates sexual development and gluconeogenesis through phosphorylation of the Zn finger transcriptional activator Rst2p in fission yeast." in: Molecular and cellular biology, Vol. 22, Issue 1, pp. 1-11, 2001 (PubMed).

Mizuno, Kawahata, Kato et al.: "STAM proteins bind ubiquitinated proteins on the early endosome via the VHS domain and ubiquitin-interacting motif." in: Molecular biology of the cell, Vol. 14, Issue 9, pp. 3675-89, 2003 (PubMed).

Mizuno, Iura, Mukai et al.: "Regulation of epidermal growth factor receptor down-regulation by UBPY-mediated deubiquitination at endosomes." in: Molecular biology of the cell, Vol. 16, Issue 11, pp. 5163-74, 2005 (PubMed).

Yamasaki, Tani, Yamamoto et al.: "The Ca2+-binding protein ALG-2 is recruited to endoplasmic reticulum exit sites by Sec31A and stabilizes the localization of Sec31A." in: Molecular biology of the cell, Vol. 17, Issue 11, pp. 4876-87, 2006 (PubMed).

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