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Microtubule-Associated Protein 1 Light Chain 3 alpha (MAP1LC3A) (AA 1-30), (N-Term) antibody

Details for Product No. ABIN1449594, Supplier: Log in to see
Antigen
  • 1010001H21Rik
  • 4922501H04Rik
  • Anx3
  • ATG8E
  • b3gnt5
  • fb12f12
  • Lc3
  • LC3
  • LC3a
  • LC3A
  • LRRGT00047
  • MAP1ALC3
  • MAP1BLC3
  • Map1lc3
  • map1lc3a
  • MAP1LC3A
  • MGC69006
  • MGC89867
  • wu:fb12f12
  • wu:fb60g11
  • wu:fe50g08
  • zgc:56434
  • zgc:77094
Epitope
AA 1-30, N-Term
43
37
28
26
23
23
14
14
14
14
13
13
12
12
11
10
8
8
7
7
6
6
5
4
3
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
317
109
103
32
30
16
14
10
9
6
6
5
3
1
1
Host
Rabbit
278
33
5
4
Clonality
Polyclonal
Conjugate
Un-conjugated
23
21
19
18
14
14
4
4
4
3
3
2
2
2
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
258
145
138
80
64
33
27
24
20
12
10
10
5
1
Supplier
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Immunogen KLH conjugated synthetic peptide between 1~30 amino acids from the N-term of Human LC3
Isotype Ig
Cross-Reactivity (Details) Species reactivity (tested):Human, Mouse, Rat.
Purification Saturated Ammonium Sulfate precipitation followed by dialysis against PBS
Alternative Name LC3 (MAP1LC3A Antibody Abstract)
Background Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). MAP1A and MAP1B are microtubule-associated proteins which mediate the physical interactions between microtubules and components of the cytoskeleton. These proteins are involved in formation of autophagosomal vacuoles (autophagosomes). MAP1A and MAP1B each consist of a heavy chain subunit and multiple light chain subunits. MAP1LC3a is one of the light chain subunits and can associate with either MAP1A or MAP1B. The precursor molecule is cleaved by APG4B/ATG4B to form the cytosolic form, LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-IISynonyms: Autophagy-related protein LC3 A, Autophagy-related ubiquitin-like modifier LC3 A, LC3A, MAP1 light chain 3-like protein 1, MAP1A / 1B light chain 3 A, MAP1A/1B light chain 3 A, MAP1A/MAP1B LC3 A, MAP1LC3A, Microtubule-associated protein 1 light chain 3 alpha, Microtubule-associated proteins 1A/1B light chain 3A
Molecular Weight 14272 Da
Gene ID 84557
NCBI Accession NM_032514
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS containing 0.09 % (W/V) Sodium Azide as preservative
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Supplier Images
Immunofluorescence (IF) image for anti-Microtubule-Associated Protein 1 Light Chain 3 alpha (MAP1LC3A) (AA 1-30), (N-Term) antibody (ABIN1449594) Immunofluorescent staining of Mouse brain cells using LC3
Immunofluorescence (IF) image for anti-Microtubule-Associated Protein 1 Light Chain 3 alpha (MAP1LC3A) (AA 1-30), (N-Term) antibody (ABIN1449594) LC3 and the CLN3-encoded protein, battenin, co-immunostaining. Wild-type and mutant c...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Microtubule-Associated Protein 1 Light Chain 3 alpha (MAP1LC3A) (AA 1-30), (N-Term) antibody (ABIN1449594) Formalin-fixed and paraffin-embedded human brain tissue reacted with Autophagy LC3 An...
Western Blotting (WB) image for anti-Microtubule-Associated Protein 1 Light Chain 3 alpha (MAP1LC3A) (AA 1-30), (N-Term) antibody (ABIN1449594) Western blot analysis of LC3 Antibody in Rat brain lysate. Both lipidated (arrow, II)...
Western Blotting (WB) image for anti-Microtubule-Associated Protein 1 Light Chain 3 alpha (MAP1LC3A) (AA 1-30), (N-Term) antibody (ABIN1449594) Western blot analysis using LC3 Antibody  in Mouse brain tissue lysates (35ug/lane).
Background publications Bains, Zaegel, Mize-Berge, Heidenreich: "IGF-I stimulates Rab7-RILP interaction during neuronal autophagy." in: Neuroscience letters, Vol. 488, Issue 2, pp. 112-7, 2011 (PubMed).

Lee, Liao, Tsai, Chiu, Wen: "Identification and anti-human glioblastoma activity of tagitinin C from Tithonia diversifolia methanolic extract." in: Journal of agricultural and food chemistry, Vol. 59, Issue 6, pp. 2347-55, 2011 (PubMed).

Nascimento-Ferreira, Santos-Ferreira, Sousa-Ferreira, Auregan, Onofre, Alves, Dufour, Colomer Gould, Koeppen, Déglon, Pereira de Almeida: "Overexpression of the autophagic beclin-1 protein clears mutant ataxin-3 and alleviates Machado-Joseph disease." in: Brain : a journal of neurology, Vol. 134, Issue Pt 5, pp. 1400-15, 2011 (PubMed).

Cirstea, Hideshima, Rodig, Santo, Pozzi, Vallet, Ikeda, Perrone, Gorgun, Patel, Desai, Sportelli, Kapoor, Vali, Mukherjee, Munshi, Anderson, Raje: "Dual inhibition of akt/mammalian target of rapamycin pathway by nanoparticle albumin-bound-rapamycin and perifosine induces antitumor activity in multiple myeloma." in: Molecular cancer therapeutics, Vol. 9, Issue 4, pp. 963-75, 2010 (PubMed).

Bohensky, Leshinsky, Srinivas, Shapiro: "Chondrocyte autophagy is stimulated by HIF-1 dependent AMPK activation and mTOR suppression." in: Pediatric nephrology (Berlin, Germany), Vol. 25, Issue 4, pp. 633-42, 2010 (PubMed).

Geng, Kohli, Klocke, Roth: "Chloroquine-induced autophagic vacuole accumulation and cell death in glioma cells is p53 independent." in: Neuro-oncology, Vol. 12, Issue 5, pp. 473-81, 2010 (PubMed).

Ma, Huang, Chen, Halliday: "Immunohistochemical evidence for macroautophagy in neurones and endothelial cells in Alzheimer's disease." in: Neuropathology and applied neurobiology, Vol. 36, Issue 4, pp. 312-9, 2010 (PubMed).

Li, Castano, Hudson, Nowlin, Lin, Bonventre, Swanson, Duffield: "The melanoma-associated transmembrane glycoprotein Gpnmb controls trafficking of cellular debris for degradation and is essential for tissue repair." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 24, Issue 12, pp. 4767-81, 2010 (PubMed).

Hino, Hamada, Tajika, Funayama, Morimura, Sakashita, Yokota, Fukamoto, Mutou, Kobayashi, Yorifuji: "Heavy ion irradiation induces autophagy in irradiated C2C12 myoblasts and their bystander cells." in: Journal of electron microscopy, Vol. 59, Issue 6, pp. 495-501, 2010 (PubMed).

Wang, Hossain, Tan, Coolman, Zhou, Liu, Casey: "Inhibition of isoprenylcysteine carboxylmethyltransferase induces autophagic-dependent apoptosis and impairs tumor growth." in: Oncogene, Vol. 29, Issue 35, pp. 4959-70, 2010 (PubMed).

Li, Ma, Wang, Liu, Yang, Zhao, Wang, Jin, Zeng, Li, Song, Li, Li, Qian, Cao: "c-Abl and Arg tyrosine kinases regulate lysosomal degradation of the oncoprotein Galectin-3." in: Cell death and differentiation, Vol. 17, Issue 8, pp. 1277-87, 2010 (PubMed).

Chen, Azad, Gibson: "Superoxide is the major reactive oxygen species regulating autophagy." in: Cell death and differentiation, Vol. 16, Issue 7, pp. 1040-52, 2009 (PubMed).

English, Chemali, Duron, Rondeau, Laplante, Gingras, Alexander, Leib, Norbury, Lippé, Desjardins: "Autophagy enhances the presentation of endogenous viral antigens on MHC class I molecules during HSV-1 infection." in: Nature immunology, Vol. 10, Issue 5, pp. 480-7, 2009 (PubMed).

Hung, Huang, Liou, Fu: "Autophagy protects neuron from Abeta-induced cytotoxicity." in: Autophagy, Vol. 5, Issue 4, pp. 502-10, 2009 (PubMed).

Johnson, Silsbee: "Thermodynamic analysis of interfacial transport and of the thermomagnetoelectric system." in: Physical review. B, Condensed matter, Vol. 35, Issue 10, pp. 4959-4972, 1999 (PubMed).