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Microtubule-Associated Protein 1 Light Chain 3 alpha (MAP1LC3A) (AA 92-121) antibody

Details for Product No. ABIN1449597, Supplier: Log in to see
Antigen
  • 1010001H21Rik
  • 4922501H04Rik
  • Anx3
  • ATG8E
  • b3gnt5
  • fb12f12
  • Lc3
  • LC3
  • LC3a
  • LC3A
  • LRRGT00047
  • MAP1ALC3
  • MAP1BLC3
  • Map1lc3
  • map1lc3a
  • MAP1LC3A
  • MGC69006
  • MGC89867
  • wu:fb12f12
  • wu:fb60g11
  • wu:fe50g08
  • zgc:56434
  • zgc:77094
Epitope
AA 92-121
43
38
28
26
24
23
14
14
14
13
13
13
12
12
11
10
8
8
7
7
6
6
5
4
3
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
317
110
104
32
30
16
14
10
9
6
6
5
3
1
1
Host
Rabbit
278
33
5
4
Clonality
Polyclonal
Conjugate
Un-conjugated
23
21
19
18
14
14
4
4
4
3
3
2
2
2
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Immunofluorescence (IF), Western Blotting (WB)
258
145
138
80
65
33
27
24
20
12
10
10
5
1
Supplier
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Immunogen KLH conjugated synthetic peptide between 92~121 amino acids from the center region of human LC3
Isotype Ig
Purification Protein A column, followed by peptide affinity purification
Alternative Name LC3 (MAP1LC3A Antibody Abstract)
Background Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). MAP1A and MAP1B are microtubule-associated proteins which mediate the physical interactions between microtubules and components of the cytoskeleton. These proteins are involved in formation of autophagosomal vacuoles (autophagosomes). MAP1A and MAP1B each consist of a heavy chain subunit and multiple light chain subunits. MAP1LC3a is one of the light chain subunits and can associate with either MAP1A or MAP1B. The precursor molecule is cleaved by APG4B/ATG4B to form the cytosolic form, LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II.Synonyms: Autophagy-related protein LC3 A, Autophagy-related ubiquitin-like modifier LC3 A, LC3A, MAP1 light chain 3-like protein 1, MAP1A / 1B light chain 3 A, MAP1A/1B light chain 3 A, MAP1A/MAP1B LC3 A, MAP1LC3A, Microtubule-associated protein 1 light chain 3 alpha, Microtubule-associated proteins 1A/1B light chain 3A
Gene ID 84557
NCBI Accession NM_032514
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS containing 0.09 % (W/V) Sodium Azide as preservative
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Supplier Images
Immunofluorescence (IF) image for anti-Microtubule-Associated Protein 1 Light Chain 3 alpha (MAP1LC3A) (AA 92-121) antibody (ABIN1449597) SY5Y cells were pretreated with 5nM bafilomycin for 24hr and fixed in methanol. Treat...
Western Blotting (WB) image for anti-Microtubule-Associated Protein 1 Light Chain 3 alpha (MAP1LC3A) (AA 92-121) antibody (ABIN1449597) Western blot analysis of LC3 (APG8a) (arrow) using Autophagy LC3 Antibody (APG8a) (D1...
Background publications Tanida, Sou, Ezaki, Minematsu-Ikeguchi, Ueno, Kominami: "HsAtg4B/HsApg4B/autophagin-1 cleaves the carboxyl termini of three human Atg8 homologues and delipidates microtubule-associated protein light chain 3- and GABAA receptor-associated protein-phospholipid conjugates." in: The Journal of biological chemistry, Vol. 279, Issue 35, pp. 36268-76, 2004 (PubMed).

He, Dang, Dai, Guo, Wu, She, Pei, Chen, Ling, Wu, Zhao, Liu, Yu: "Post-translational modifications of three members of the human MAP1LC3 family and detection of a novel type of modification for MAP1LC3B." in: The Journal of biological chemistry, Vol. 278, Issue 31, pp. 29278-87, 2003 (PubMed).

Mann, Hammarback: "Gene localization and developmental expression of light chain 3: a common subunit of microtubule-associated protein 1A(MAP1A) and MAP1B." in: Journal of neuroscience research, Vol. 43, Issue 5, pp. 535-44, 1997 (PubMed).