Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B) (C-Term), (AA 96-125), (cleaved) antibody

Details for Product No. ABIN1449601
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Antigen
Synonyms ATG8F, LC3B, MAP1A/1BLC3, MAP1LC3B-a, Map1lc3, Mpl3, zbs559, 1010001C15Rik, Atg8, LC3b, zgc:56434, wu:fb60g11, map1lc3b, MGC76283, MAP1LC3B
Epitope
C-Term, AA 96-125, cleaved
(25), (11), (9), (9), (6), (6), (6), (5), (5), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Reactivity
Human, Mouse (Murine)
(117), (48), (39), (25), (25), (14), (14), (4), (2), (1), (1), (1)
Host
Rabbit
(106), (14)
Clonality
Polyclonal
Conjugate
Un-conjugated
(9), (7), (7), (7), (6), (5), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), ELISA
(63), (60), (32), (23), (22), (20), (17), (16), (11), (4), (2), (1), (1)
Pubmed 11 references available
Catalog no. ABIN1449601
Quantity 0.4 mL
Price
357.50 $   Plus shipping costs $45.00
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Availability Will be delivered in 6 to 8 Business Days

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  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen KLH conjugated synthetic peptide between 96~125 amino acids from the C-terminal region of Human cleaved-LC3
Specificity This antibody recognizes Cleaved Human and Mouse LC3B. Other species not tested.
Purification Saturated Ammonium Sulfate precipitation followed by dialysis against PBS
Alternative Name LC3B
Background Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). MAP1A and MAP1B are microtubule-associated proteins which mediate the physical interactions between microtubules and components of the cytoskeleton. These proteins are involved in formation of autophagosomal vacuoles (autophagosomes). MAP1A and MAP1B each consist of a heavy chain subunit and multiple light chain subunits. MAP1LC3b is one of the light chain subunits and can associate with either MAP1A or MAP1B. The precursor molecule is cleaved by APG4B/ATG4B to form the cytosolic form, LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane- bound form, LC3-II.
Alternate names: Autophagy-related protein LC3 B, Autophagy-related ubiquitin-like modifier LC3 B, MAP1A / 1B light chain 3 B, MAP1A/MAP1B, MAP1LC3B, Map1alc3, Map1lc3, Map1lc3b, Microtubule- associated protein 1 light chain 3 beta, Microtubule-associated proteins 1A/1B light chain 3B
Gene ID 81631
NCBI Accession NP_073729.1
Research Area Cancer, Autophagy, Cytoskeleton
Restrictions For Research Use only
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS containing 0.09% (w/v) Sodium Azide as preservative
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Expiry Date 12 months
Background publications He, Dang, Dai et al.: "Post-translational modifications of three members of the human MAP1LC3 family and detection of a novel type of modification for MAP1LC3B." in: The Journal of biological chemistry, Vol. 278, Issue 31, pp. 29278-87, 2003 (PubMed).

Tanida, Sou, Ezaki et al.: "HsAtg4B/HsApg4B/autophagin-1 cleaves the carboxyl termini of three human Atg8 homologues and delipidates microtubule-associated protein light chain 3- and GABAA receptor-associated protein-phospholipid conjugates." in: The Journal of biological chemistry, Vol. 279, Issue 35, pp. 36268-76, 2004 (PubMed).

Tanida, Ueno, Kominami: "LC3 conjugation system in mammalian autophagy." in: The international journal of biochemistry & cell biology, Vol. 36, Issue 12, pp. 2503-18, 2004 (PubMed).

Shintani, Klionsky: "Autophagy in health and disease: a double-edged sword." in: Science (New York, N.Y.), Vol. 306, Issue 5698, pp. 990-5, 2004 (PubMed).

Levine: "Eating oneself and uninvited guests: autophagy-related pathways in cellular defense." in: Cell, Vol. 120, Issue 2, pp. 159-62, 2005 (PubMed).

Lum, DeBerardinis, Thompson: "Autophagy in metazoans: cell survival in the land of plenty." in: Nature reviews. Molecular cell biology, Vol. 6, Issue 6, pp. 439-48, 2005 (PubMed).

Baehrecke: "Autophagy: dual roles in life and death?" in: Nature reviews. Molecular cell biology, Vol. 6, Issue 6, pp. 505-10, 2005 (PubMed).

Greenberg: "Degrade or die: a dual function for autophagy in the plant immune response." in: Developmental cell, Vol. 8, Issue 6, pp. 799-801, 2005 (PubMed).

English, Chemali, Duron et al.: "Autophagy enhances the presentation of endogenous viral antigens on MHC class I molecules during HSV-1 infection." in: Nature immunology, Vol. 10, Issue 5, pp. 480-7, 2009 (PubMed).

Parikh, Childress, Deitrick et al.: "Statin-induced autophagy by inhibition of geranylgeranyl biosynthesis in prostate cancer PC3 cells." in: The Prostate, Vol. 70, Issue 9, pp. 971-81, 2010 (PubMed).

Checinska, Soengas: "The gluttonous side of malignant melanoma: basic and clinical implications of macroautophagy." in: Pigment cell & melanoma research, Vol. 24, Issue 6, pp. 1116-32, 2011 (PubMed).

Hosts (106), (14)
Reactivities (117), (48), (39), (25), (25), (14), (14), (4), (2), (1), (1), (1)
Applications (63), (60), (32), (23), (22), (20), (17), (16), (11), (4), (2), (1), (1)
Conjugates (9), (7), (7), (7), (6), (5), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Epitopes (25), (11), (9), (9), (6), (6), (6), (5), (5), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Validation Images
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