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Microtubule-Associated Protein 1 Light Chain 3 alpha (MAP1LC3A) (AA 1-30), (pSer12), (N-Term) antibody

Details for Product No. ABIN1449623
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Antigen
Synonyms
MGC69006, MGC89867, MAP1LC3A, map1lc3a, Anx3, LC3, LRRGT00047, ATG8E, LC3A, MAP1ALC3, MAP1BLC3, 1010001H21Rik, 4922501H04Rik, LC3a, zgc:77094, Map1lc3, zgc:56434, wu:fb60g11, Lc3, b3gnt5, fb12f12, wu: ... show more
MGC69006, MGC89867, MAP1LC3A, map1lc3a, Anx3, LC3, LRRGT00047, ATG8E, LC3A, MAP1ALC3, MAP1BLC3, 1010001H21Rik, 4922501H04Rik, LC3a, zgc:77094, Map1lc3, zgc:56434, wu:fb60g11, Lc3, b3gnt5, fb12f12, wu:fb12f12, wu:fe50g08 show less
Epitope
AA 1-30, pSer12, N-Term
(23), (21), (13), (11), (10), (10), (9), (7), (6), (6), (6), (5), (5), (5), (4), (4), (4), (4), (3), (3), (3), (2), (2), (2), (1), (1), (1)
Reactivity
Human
(174), (84), (74), (42), (25), (25), (15), (12), (3), (3), (1), (1), (1)
Host
Rabbit
(152), (19), (4), (3)
Clonality
Polyclonal
Conjugate
Un-conjugated
(13), (10), (10), (10), (7), (7), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2)
Application
Dot Blot (DB), ELISA, Western Blotting (WB)
(138), (68), (59), (39), (30), (30), (20), (15), (15), (10), (2), (2), (1)
Pubmed 9 references available
Quantity 0.4 mL
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Catalog No. ABIN1449623
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Immunogen KLH conjugated synthetic phosphopeptide between 1-30 amino acids surrounding Ser12 of Human LC3
Specificity Recognizes Phospho LC3C- Serine12
Purification Protein A Affinity Chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with peptides. The antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS
Alternative Name LC3
Background MAP1A and MAP1B are microtubule-associated proteins which mediate the physical interactions between microtubules and components of the cytoskeleton. These proteins are involved in formation of autophagosomal vacuoles (autophagosomes). MAP1A and MAP1B each consist of a heavy chain subunit and multiple light chain subunits. MAP1LC3a is one of the light chain subunits and can associate with either MAP1A or MAP1B. The precursor molecule is cleaved by APG4B/ATG4B to form the cytosolic form, LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II. Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole).
Alternate names: Autophagy-related protein LC3 A, Autophagy-related ubiquitin-like modifier LC3 A, LC3A, MAP1 light chain 3-like protein 1, MAP1A / 1B light chain 3 A, MAP1A/1B light chain 3 A, MAP1A/MAP1B LC3 A, MAP1LC3A, Microtubule-associated protein 1 light chain 3 alpha, Microtubule-associated proteins 1A/1B light chain 3A
NCBI Accession NM_032514.2
Restrictions For Research Use only
Format Liquid
Buffer PBS 0.09% (w/v) Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Expiry Date 12 months
Product cited in: Su, Chao, Huang et al.: "Rab5 and class III phosphoinositide 3-kinase Vps34 are involved in hepatitis C virus NS4B-induced autophagy." in: Journal of virology, Vol. 85, Issue 20, pp. 10561-71, 2011 (PubMed).

Background publications He, Dang, Dai et al.: "Post-translational modifications of three members of the human MAP1LC3 family and detection of a novel type of modification for MAP1LC3B." in: The Journal of biological chemistry, Vol. 278, Issue 31, pp. 29278-87, 2003 (PubMed).

Tanida, Sou, Ezaki et al.: "HsAtg4B/HsApg4B/autophagin-1 cleaves the carboxyl termini of three human Atg8 homologues and delipidates microtubule-associated protein light chain 3- and GABAA receptor-associated protein-phospholipid conjugates." in: The Journal of biological chemistry, Vol. 279, Issue 35, pp. 36268-76, 2004 (PubMed).

Tanida, Ueno, Kominami: "LC3 conjugation system in mammalian autophagy." in: The international journal of biochemistry & cell biology, Vol. 36, Issue 12, pp. 2503-18, 2004 (PubMed).

Shintani, Klionsky: "Autophagy in health and disease: a double-edged sword." in: Science (New York, N.Y.), Vol. 306, Issue 5698, pp. 990-5, 2004 (PubMed).

Levine: "Eating oneself and uninvited guests: autophagy-related pathways in cellular defense." in: Cell, Vol. 120, Issue 2, pp. 159-62, 2005 (PubMed).

Lum, DeBerardinis, Thompson: "Autophagy in metazoans: cell survival in the land of plenty." in: Nature reviews. Molecular cell biology, Vol. 6, Issue 6, pp. 439-48, 2005 (PubMed).

Baehrecke: "Autophagy: dual roles in life and death?" in: Nature reviews. Molecular cell biology, Vol. 6, Issue 6, pp. 505-10, 2005 (PubMed).

Greenberg: "Degrade or die: a dual function for autophagy in the plant immune response." in: Developmental cell, Vol. 8, Issue 6, pp. 799-801, 2005 (PubMed).

Validation Images
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