Nicalin antibody (C-Term)

Catalog No. ABIN1450143

Product Details anti-Nicalin Antibody

Target: Nicalin (NCLN)

Binding Specificity: C-Term

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Nicalin antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Specificity: Multiple isoforms of NCLN are known to exist.

Cross-Reactivity (Details): Species reactivity (tested):Human, Mouse, Rat.

Purification: Affinity chromatography purified via peptide column

Immunogen: 18 amino acid synthetic peptide near the carboxy terminus of Human NCLN

Isotype: IgG

Application Details

Application Notes: Western blot: 1-2 μg/ml.
Positive Control: Mouse heart tissue lysate.
Immunofluorescence: Start at 2.5 μg/ml.
Immunohistochemistry on Paraffin Sections: Start at 2.5 μg/ml.

Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS containing 0.02 % Sodium Azide as preservative

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for Nicalin

Target: Nicalin (NCLN)

Alternative Name: Nicalin (NCLN Products)

Synonyms: NET59 antibody, RGD1309355 antibody, wu:fb07e09 antibody, wu:fk52a06 antibody, zgc:77236 antibody, 3100002P13Rik antibody, AW120537 antibody, nicalin antibody, NCLN antibody, Bm1_29425 antibody, LOC100280679 antibody, Ncln antibody, ncln antibody

Background: Nicalin, along with its binding partner Nomo, is a novel signaling antagonist to Nodals, signaling factors of the TGF-beta superfamily that play a key role in vertebrate development. Nicalin is distantly related to Nicastrin, a component of the Alzheimer’s disease-associated gamma-secretase, and is thought to have a similar function. Another protein, TMEM147, has also been recently identified as associating with Nicalin and NOMO as part of the gamma-secretase-like complex.
Synonyms: NCLN

Gene ID: 56926

NCBI Accession: NP_064555

UniProt: Q969V3