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Telomerase Reverse Transcriptase (TERT) (AA 1104-1123), (C-Term) antibody

Details for Product No. ABIN1450190, Supplier: Login to see
Antigen
  • Tert
  • tert
  • CMM9
  • DKCA2
  • DKCB4
  • EST2
  • PFBMFT1
  • TCS1
  • TP2
  • TRT
  • hEST2
  • hTRT
  • TR
  • chTERT
  • ATTERT
  • F5E19.190
  • F5E19_190
  • telomerase reverse transcriptase
Alternatives
anti-Human Telomerase Reverse Transcriptase antibody for Immunofluorescence
Epitope
C-Term, AA 1104-1123
22
18
16
13
13
13
11
9
9
8
8
7
6
5
5
5
4
4
3
3
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
215
54
43
4
2
Host
Rabbit
220
13
6
2
Clonality
Polyclonal
Conjugate
Un-conjugated
16
15
13
7
7
7
4
3
3
3
3
3
3
3
3
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
131
122
38
35
31
27
16
13
11
6
4
3
3
1
Supplier
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Immunogen Synthetic peptide corresponding to amino acids 1104-1123 of the carboxy terminal end of Human TERT
Sequence S-R-K-L-P-G-T-T-L-T-A-L-E-A-A-A-N-P-A-L Remarks: Accession Number: AF018167
Specificity This antiserum primarily detects Human TERT, but several non-specific bands appear on immunoblots (Figure 1). In Immunofluorescence Microscopy assays, staining with anti-TERT-16 was specific to the nuclei of cells with ectopic TERT expression.
Cross-Reactivity (Details) Species reactivity (tested):Human.
Purification Affinity Chromatography
Alternative Name TERT (TERT Antibody Abstract)
Background Telomerase is a reverse transcriptase that adds telomeric repeats (TTAGGG)n to chromosomal ends, compensating for the telomere shortening that occurs with DNA replication. In normal human somatic cells, telomerase is repressed and telomeres progressively shorten, leading to limited lifespan and senescence. Reactivation of telomerase activity is associated with human cancer and cell immortalization. Approximately 85 % of human cancers, including breast, prostate, stomach, bladder, colon, and liver cancer, have telomerase activity, whereas most normal somatic cells do not. The specificity of telomerase to human cancer has led to investigations of telomerase activity and expression as a tumor marker. For example, the presence of telomerase activity in human urine has been identified as a marker for human bladder carcinoma. Human telomerase consists of three major subunits: a catalytic protein subunit called hTERT (for human TElomerase Reverse Transcriptase), a template RNA called hTR, and telomerase-associated protein (TEP-1). TERT and hTR are minimally required to reconstitute telomerase activity in vitro. In human cells, hTR is constitutively expressed. TERT transcription is a primary mechanism for regulation of telomerase activity.Synonyms: EST2, HEST2, TCS1, TRT, Telomerase catalytic subunit, Telomerase reverse transcriptase, Telomerase-associated protein 2
Gene ID 7015
NCBI Accession NP_001180305
UniProt O14746
Research Area DNA/RNA, Cell Cycle, Enzymes
Pathways Telomere Maintenance
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Concentration 1.0 mg/mL (by UV absorbance at 280 nm).
Buffer 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2, 0.01 % (w/v) Sodium Azide, None
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the lyophilized product at 2-8 °C. Following reconstitution store in aliquots at -20 °C.
Product cited in: van den Akker, Surtel, Cremers et al.: "Novel immortal human cell lines reveal subpopulations in the nucleus pulposus." in: Arthritis research & therapy, Vol. 16, Issue 3, pp. R135, 2014 (PubMed).

Ahmed, Passos, Birket et al.: "Telomerase does not counteract telomere shortening but protects mitochondrial function under oxidative stress." in: Journal of cell science, Vol. 121, Issue Pt 7, pp. 1046-53, 2008 (PubMed).

Background publications Wu, Dudognon, Nguyen et al.: "Immunodetection of human telomerase reverse-transcriptase (hTERT) re-appraised: nucleolin and telomerase cross paths." in: Journal of cell science, Vol. 119, Issue Pt 13, pp. 2797-806, 2006 (PubMed).

Drissi, Zindy, Roussel et al.: "c-Myc-mediated regulation of telomerase activity is disabled in immortalized cells." in: The Journal of biological chemistry, Vol. 276, Issue 32, pp. 29994-30001, 2001 (PubMed).

Shay, Zou, Hiyama et al.: "Telomerase and cancer." in: Human molecular genetics, Vol. 10, Issue 7, pp. 677-85, 2001 (PubMed).