Telomerase Reverse Transcriptase (TERT) (C-Term), (AA 1104-1123) antibody

Details for Product No. ABIN1450190
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Antigen
Synonyms chTERT, CMM9, DKCA2, DKCB4, EST2, PFBMFT1, TCS1, TP2, TRT, hEST2, hTRT, TR, Tert, ATTERT, F5E19.190, F5E19_190, telomerase reverse transcriptase, tert
Epitope
C-Term, AA 1104-1123
(17), (9), (7), (6), (5), (4), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(117), (44), (42), (2), (1), (1)
Host
Rabbit
(112), (10), (6), (2), (1)
Clonality
Polyclonal
Conjugate
Un-conjugated
(4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4)
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
(71), (51), (40), (25), (22), (13), (9), (8), (6), (6), (4), (2), (2), (1)
Pubmed 4 references available
Catalog no. ABIN1450190
Quantity 0.1 mg
Price
390.50 $   Plus shipping costs $45.00
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  • +1 404 474 4654
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Immunogen Synthetic peptide corresponding to amino acids 1104-1123 of the carboxy terminal end of Human TERT
Sequence S-R-K-L-P-G-T-T-L-T-A-L-E-A-A-A-N-P-A-L Remarks: Accession Number: AF018167
Specificity This antiserum primarily detects Human TERT, but several non-specific bands appear on immunoblots (Figure 1). In Immunofluorescence Microscopy assays, staining with anti-TERT-16 was specific to the nuclei of cells with ectopic TERT expression. Species Reactivity: Tested: Human.
Purification Affinity Chromatography
Alternative Name TERT
Background Telomerase is a reverse transcriptase that adds telomeric repeats (TTAGGG)n to chromosomal ends, compensating for the telomere shortening that occurs with DNA replication. In normal human somatic cells, telomerase is repressed and telomeres progressively shorten, leading to limited lifespan and senescence. Reactivation of telomerase activity is associated with human cancer and cell immortalization. Approximately 85% of human cancers, including breast, prostate, stomach, bladder, colon, and liver cancer, have telomerase activity, whereas most normal somatic cells do not. The specificity of telomerase to human cancer has led to investigations of telomerase activity and expression as a tumor marker. For example, the presence of telomerase activity in human urine has been identified as a marker for human bladder carcinoma. Human telomerase consists of three major subunits: a catalytic protein subunit called hTERT (for human TElomerase Reverse Transcriptase), a template RNA called hTR, and telomerase- associated protein (TEP-1). TERT and hTR are minimally required to reconstitute telomerase activity in vitro. In human cells, hTR is constitutively expressed. TERT transcription is a primary mechanism for regulation of telomerase activity.
Alternate names: EST2, HEST2, TCS1, TRT, Telomerase catalytic subunit, Telomerase reverse transcriptase, Telomerase-associated protein 2
Gene ID 7015
NCBI Accession NP_001180305.1
UniProt O14746
Research Area DNA/RNA, Cell Cycle, Enzymes
Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL (by UV absorbance at 280 nm).
Buffer 0.02M Potassium Phosphate, 0.15M Sodium Chloride, pH 7.2 0.01% (w/v) Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the lyophilized product at 2-8°C. Following reconstitution store in aliquots at -20°C.
Expiry Date 12 months
Product cited in: Ahmed, Passos, Birket et al.: "Telomerase does not counteract telomere shortening but protects mitochondrial function under oxidative stress." in: Journal of cell science, Vol. 121, Issue Pt 7, pp. 1046-53, 2008 (PubMed).

Background publications Shay, Zou, Hiyama et al.: "Telomerase and cancer." in: Human molecular genetics, Vol. 10, Issue 7, pp. 677-85, 2001 (PubMed).

Drissi, Zindy, Roussel et al.: "c-Myc-mediated regulation of telomerase activity is disabled in immortalized cells." in: The Journal of biological chemistry, Vol. 276, Issue 32, pp. 29994-30001, 2001 (PubMed).

Wu, Dudognon, Nguyen et al.: "Immunodetection of human telomerase reverse-transcriptase (hTERT) re-appraised: nucleolin and telomerase cross paths." in: Journal of cell science, Vol. 119, Issue Pt 13, pp. 2797-806, 2006 (PubMed).

Hosts (112), (10), (6), (2), (1)
Reactivities (117), (44), (42), (2), (1), (1)
Applications (71), (51), (40), (25), (22), (13), (9), (8), (6), (6), (4), (2), (2), (1)
Conjugates (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4)
Epitopes (17), (9), (7), (6), (5), (4), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Validation Images
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