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Collagen, Type I (COL1) antibody

Details for Product No. ABIN145176, Supplier: Login to see
Antigen
Reactivity
Cow (Bovine)
188
63
54
25
18
10
9
9
7
6
4
3
2
2
2
2
2
2
1
1
Host
Mouse
194
54
41
2
1
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
32
7
5
3
3
3
2
1
1
1
1
1
1
1
1
1
Application
Dot Blot (DB), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), ELISA
191
104
100
85
83
71
69
43
28
28
25
15
14
8
5
4
4
2
1
1
1
1
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Immunogen Full length native protein (purified) (Cow).
Clone COL-1
Isotype IgG1
Specificity Recognises the native (helical) form of collagen type I using ELISA and dot-blot. Does not react with the thermally-denatured molecule. The antibody shows no cross-reactivity with collagen types II, III, IV, V, VI, VII, IX, X and XI.
Cross-Reactivity Human, Deer, Pig (Porcine), Rabbit, Rat (Rattus)
Purification Unpurified. Ascites
Alternative Name Collagen type I
Background Collagens are highly conserved throughout evolution and are characterised by an uninterrupted "Glycine X Y" triplet repeat that is a necessary part of the triple helical structure. Type I collagen (95 kDa) is found in bone, cornea, skin and tendon. Mutations in the encoding gene are associated with osteogenesis imperfecta, Ehlers Danlos syndrome, and idiopathic osteoporosis. Reciprocal translocations between chromosomes 17 and 22, where this gene and the gene for Platelet-derived growth factor beta are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans, resulting from unregulated expression of the growth factor.
Synonyms: MGC128258, 282187, COL1A1, COL1A2, 282188, Collagen I
Research Area Extracellular Matrix
Pathways
Application Notes Dot blot, ELISA, ICC/IF, IHC (Frozen sections). The usefulness of this product in other applications has not been determined. For IF: Use at a dilution of 1:2,000. For IHC-Fr: Use at a dilution of 1:2,000. Optimal dilutions/concentrations should be determined by the researcher.
Restrictions For Research Use only
Format Liquid
Buffer Phosphate-buffered saline, pH 7.2, no preservative added.
Preservative Without preservative
Handling Advice Avoid freeze-thaw cycles.
Storage 4 °C
Storage Comment Keep as concentrated solution. Store at 4°C short term. For extended storage aliquot and store at -20°C or below.
Supplier Images
 image for anti-Collagen, Type I (COL1) antibody (ABIN145176) anti-Collagen, Type I (COL1) antibody
 image for anti-Collagen, Type I (COL1) antibody (ABIN145176) anti-Collagen, Type I (COL1) antibody (Image 2)
Background publications Gay, Fine: "Characterization and isolation of poly- and monoclonal antibodies against collagen for use in immunohistochemistry." in: Methods in enzymology, Vol. 145, pp. 148-67, 1987 (PubMed).

Mayne: "Preparation and applications of monoclonal antibodies to different collagen types." in: Clinical biochemistry, Vol. 21, Issue 2, pp. 111-5, 1988 (PubMed).

Werkmeister, Ramshaw: "Monoclonal antibodies to collagens for immunofluorescent examination of human skin." in: Acta dermato-venereologica, Vol. 69, Issue 5, pp. 399-402, 1989 (PubMed).

Sanes, Engvall, Butkowski et al.: "Molecular heterogeneity of basal laminae: isoforms of laminin and collagen IV at the neuromuscular junction and elsewhere." in: The Journal of cell biology, Vol. 111, Issue 4, pp. 1685-99, 1990 (PubMed).