Spectrin alpha Chain (Erythrocyte) / SPTA1 (Alpha 1) (SH3 Domain) antibody

Details for Product No. ABIN145343
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Antigen
Epitope
SH3 Domain
Reactivity
Human
Host
Mouse
Clonality
Monoclonal
Application
Immunofluorescence (IF), Western Blotting (WB), Immunohistochemistry (IHC), ELISA
Pubmed 2 references available
Catalog no. ABIN145343
Quantity 0.1 mL
Price
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Immunogen Recombinant alpha I spectrin (erythroid spectrin) SH3 domain.
Isotype IgG1
Specificity ABIN145343 is developed against the alpha I spectrin SH3 domain. It does not crossreact with alpha II (nonerythroid) spectrin SH3 domain or recombinant human fodrin SH3 domain. In NIH 3T3 cells, ABIN145343 reacts with a 200 kDa protein which is a candidate ma
Cross-Reactivity Mouse (Murine), Rat (Rattus)
Purification Purified
Background Spectrin is the predominant component of the membrane skeleton of the red blood cell and is essential in determining shape and deformability of the membrane. It consists of 2 nonidentical subunits, alpha (MW 240,000) and beta (MW 225,000). Spectrin is present in the red cell membrane in a tetrameric or possibly higher polymeric form through head-to-head self-association of heterodimers that are linked by actin polymers and protein 4.1 to form a 2-dimensional network.
Application Notes ELISA: Use at a dilution of 1/100000-1/1000000. IF: Use at a dilution of 1/100-1/500. IHC-Fr: Use at a dilution of 1/10-1/50. WB: Use at a dilution of 1/100-1/1000. Predicted molecular weight: 280 kDa. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/ml
Buffer PBS, Ammonium sulphate
Preservative Sodium azide
Storage 4 °C
General Lefkowitz: "Identification of adenylate cyclase-coupled beta-adrenergic receptors with radiolabeled beta-adrenergic antagonists." in: Biochemical pharmacology, Vol. 24, Issue 18, pp. 1651-8, 1976 (PubMed).

Xu, Ziemnicka, Merz et al.: "Human spectrin Src homology 3 domain binding protein 1 regulates macropinocytosis in NIH 3T3 cells." in: Journal of cell science, Vol. 113 Pt 21, pp. 3805-14, 2000 (PubMed).

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