Spectrin alpha chain (erythrocyte) / SPTA1 (alpha 1) (SH3 Domain) antibody

Antigen: Spectrin alpha chain (erythrocyte) / SPTA1 (alpha 1)

Binding Site: SH3 Domain

Clonality: Monoclonal

Host: Mouse

Reactivity: Human

Application: Immunofluorescence (IF), Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Catalog no.: ABIN145343

Additional Information

Immunogen: Recombinant alpha I spectrin (erythroid spectrin) SH3 domain.

Cross-Reactivity: Human, Mouse (Murine), Rat (Rattus)

Isotype: IgG1

Description: Spectrin is the predominant component of the membrane skeleton of the red blood cell and is essential in determining shape and deformability of the membrane. It consists of 2 nonidentical subunits, alpha (MW 240,000) and beta (MW 225,000). Spectrin is present in the red cell membrane in a tetrameric or possibly higher polymeric form through head-to-head self-association of heterodimers that are linked by actin polymers and protein 4.1 to form a 2-dimensional network.

Specificity: ABIN145343 is developed against the alpha I spectrin SH3 domain. It does not crossreact with alpha II (nonerythroid) spectrin SH3 domain or recombinant human fodrin SH3 domain. In NIH 3T3 cells, ABIN145343 reacts with a 200 kDa protein which is a candidate ma

Application Details

Application Notes: ELISA: Use at a dilution of 1/100000-1/1000000. IF: Use at a dilution of 1/100-1/500. IHC-Fr: Use at a dilution of 1/10-1/50. WB: Use at a dilution of 1/100-1/1000. Predicted molecular weight: 280 kDa. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

Concentration: 1 mg/ml

Purification: Purified

Buffer: PBS, Ammonium sulphate

Preservative: 0.1% Sodium Azide

Storage: Store at 4 C. Aliquot and store at -20 C long-term.

Restrictions: For Research Use only

Publications

Lefkowitz: "Identification of adenylate cyclase-coupled beta-adrenergic receptors with radiolabeled beta-adrenergic antagonists." in: Biochemical pharmacology, Vol. 24, Issue 18, pp. 1651-8, 1976 (PubMed).

Xu, Ziemnicka, Merz et al.: "Human spectrin Src homology 3 domain binding protein 1 regulates macropinocytosis in NIH 3T3 cells." in: Journal of cell science, Vol. 113 Pt 21, pp. 3805-14, 2000 (PubMed).