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Cytokeratin 1 (KRT1) (pan) antibody

Details for Product No. ABIN145492
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Antigen
Synonyms EpK, cytok, cytokeratin, epi-keratin, epiker, xK81
Epitope
pan
(22), (7)
Reactivity
Human
(44), (7), (7), (3), (3), (2), (2), (1), (1), (1), (1), (1)
Host
Mouse
(44), (1), (1)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(1), (1), (1)
Application
Immunohistochemistry (Acetone-fixed) (IHC (af)), Dot Blot (DB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(29), (19), (18), (15), (13), (11), (4), (2), (2), (1)
Pubmed 3 references available
Quantity 0.1 mL
Shipping to United States (Change)
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Catalog No. ABIN145492
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Immunogen Full length native protein (purified) (Human epidermis).
Clone PCK-26
Isotype IgG1
Specificity PCK-26 recognizes the 58 kDa cytokeratin 5, the 56 kDa cytokeratin 6 and the 52 kDa cytokeratin 8 band in immunoblotting (Lane EB et al). It reacts specifically with a wide variety of normal, reactive and neoplastic epithelial tissues. The antibody reacts with simple, cornifying and non-cornifying squamous epithelia and pseudostratified epithelia. It does not react with non-epithelial normal human tissues.
Cross-Reactivity Mouse (Murine), Cow (Bovine), Goat, Rabbit, Rat (Rattus), Sheep (Ovine)
Purification Ascites.
Alternative Name Cytokeratin
Background Cytokeratins, a group comprising at least 29 different proteins, are characteristic of epithelial and trichocytic cells. Cytokeratin 5, 6 and 8 are members of the type II neutral-to-basic subfamily. Monoclonal antibodies to cytokeratins are specific markers of epithelial cell differentiation and have been widely used as tools in tumor identification and classification. PCK-26 facilitates the typing of normal, metaplastic and neoplastic cells and it may aid in the discrimination of carcinomas and non-epithelial tumors such as sarcomas, lymphomas and neural tumors. It is also useful in detecting micro-metastases in lymph nodes and other tissues and for determining the origin of poorly differentiated tumors (1,2).
Research Area Extracellular Matrix
Application Notes AF: Use at an assay dependant dilution. Dot: Use at an assay dependant dilution. IHC-F: Use at an assay dependant dilution. IF: Use at a dilution of 1/300. IHC-P: Use at an assay dependant dilution. IHC-Fr: Use at an assay dependant dilution. WB: Use at an assay dependant dilution. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Restrictions For Research Use only
Format Liquid
Buffer Ascitic fluid with 0.1% sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles.
Storage 4 °C
Storage Comment Keep as concentrated solution. Store at 4°C short term. For extended storage aliquot and store at -20°C or below.
General Lane, Rugg, Navsaria et al.: "A mutation in the conserved helix termination peptide of keratin 5 in hereditary skin blistering." in: Nature, Vol. 356, Issue 6366, pp. 244-6, 1992 (PubMed).

Lane, Alexander: "Use of keratin antibodies in tumor diagnosis." in: Seminars in cancer biology, Vol. 1, Issue 3, pp. 165-79, 1991 (PubMed).

Moll, Franke, Schiller et al.: "The catalog of human cytokeratins: patterns of expression in normal epithelia, tumors and cultured cells." in: Cell, Vol. 31, Issue 1, pp. 11-24, 1983 (PubMed).

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