Cadherin (pan) antibody

Antigen: Cadherin

Synonyms: PCDH10, OLpcad, cadherin, ci-cadherin

Binding Site: pan

Clonality: Monoclonal

Host: Mouse

Reactivity: Chicken

Application: Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Catalog no.: ABIN145920

Additional Information

Immunogen: Synthetic peptide: DYDYLNDWGPRFKKLADMYGGGDD conjugated to KLH, corresponding to amino acids 889-912 of Chicken N-Cadherin (cdh2).

Cross-Reactivity: Chicken, Goat, Hamster, Human, Mouse (Murine), Rabbit, Rat (Rattus), Sheep (Ovine), Xenopus laevis

Isotype: IgG1

Description: Mouse monoclonal [CH - 19] to pan Cadherin - Plasma Membrane Marker. Background: This product is useful for the detection of members of the cadherin family or genetically engineered proteins containing the C-terminal cadherin tail, and for demonstration of adherens type cell-cell junctions regardless of their cadherin type.

Specificity: Reacts with all cadherins. Cross Reactivity: Cross-reacts with Human, Mouse, Rat, Cat, Chicken, Cow, Dog, Goat, Guinea Pig, Hamster, Pig, Psammoys (sand rat), Rabbit, Sheep, Snake and Xenopus. Not yet tested in other species.

Application Details

Application Notes: IF: Use at a dilution of 1/500 using cultured MDBK cells. IHC-P: Use at a dilution of 1/500. Perform enzymatic antigen retrieval before commencing with IHC staining protocol (Formalin-fixed paraffin-embedded sections). WB: Use at an assay dependant dilution. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

Purification: Ascites

Buffer: Ascitic fluid with 0.1% sodium azide

Storage: Store at 4 °C. Aliquot and store at -20 °C long-term. Avoid repeated freeze / thaw cycles.

Research Area: Extracellular Matrix, Signaling, Cytoskeleton

Restrictions: For Research Use only

Images

anti-Cadherin (pan) antibody

Publications

Takeichi: "Cadherin cell adhesion receptors as a morphogenetic regulator." in: Science (New York, N.Y.), Vol. 251, Issue 5000, pp. 1451-5, 1991 (PubMed).

Kartenbeck, Schmelz, Franke et al.: "Endocytosis of junctional cadherins in bovine kidney epithelial (MDBK) cells cultured in low Ca2+ ion medium." in: The Journal of cell biology, Vol. 113, Issue 4, pp. 881-92, 1991 (PubMed).

Geiger, Volberg, Ginsberg et al.: "Broad spectrum pan-cadherin antibodies, reactive with the C-terminal 24 amino acid residues of N-cadherin." in: Journal of cell science, Vol. 97 ( Pt 4), pp. 607-14, 1991 (PubMed).

Takeichi: "Cadherins: a molecular family important in selective cell-cell adhesion." in: Annual review of biochemistry, Vol. 59, pp. 237-52, 1990 (PubMed).

Takeichi: "The cadherins: cell-cell adhesion molecules controlling animal morphogenesis." in: Development (Cambridge, England), Vol. 102, Issue 4, pp. 639-55, 1988 (PubMed).

Grunwald: "The structural and functional analysis of cadherin calcium-dependent cell adhesion molecules." in: Current opinion in cell biology, Vol. 5, Issue 5, pp. 797-805, 1993 (PubMed).

Takeichi: "Cadherins in cancer: implications for invasion and metastasis." in: Current opinion in cell biology, Vol. 5, Issue 5, pp. 806-11, 1993 (PubMed).