Antigen Identified By Monoclonal antibody Ki-67 (MKI67) antibody

Antigen: Antigen Identified By Monoclonal Antibody Ki-67 (MKI67)

Synonyms: KIA, Ki67, Ki-67, D630048A14Rik, MKI67, kia, mki67, MGC132156

Clonality: Monoclonal

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB), Immunohistochemistry (IHC)

Catalog no.: ABIN145923

Additional Information

Alternative name: Ki-67

Immunogen: A HeLa cell nuclear preparation.

Cross-Reactivity: Human

Isotype: IgM

Description: Ki-67 antigen is the prototypic cell cycle related nuclear protein, expressed by proliferating cells in all phases of the active cell cycle (G1, S, G2 and M phase). It is absent in resting (G0) cells. The monoclonal antibody is useful in establishing the cell growing fraction in neoplasms (immunohistochemically quantified by determining the number of Ki67-positive cells among the total number of resting cells = Ki67 index). In neoplastic tissues the prognostic value is comparable to the tritiated thymidine-labeling index. The correlation between low Ki67 index and histologically low-grade tumors is strong.

Specificity: Monoclonal Anti-Proliferating Cell Protein Ki-67 reacts with the Ki-67 nuclear antigen (345 and 395 kD, and several lower molecular weight proteins) in immuno-blotting. The product shows an immunohistochemical staining pattern similar to that obtained wit

Application Details

Application Notes: Recommended dilutions: ICC (IF): 1/200(see review). Fix with 4%PFA. IHC-P: 1/800. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol. IHC-Fr: 1/800 (see review). Fix with formalin. WB: Use at an assay dependant dilution. For immunohisto/cytochemical protocols permeabilisation may be necessary (e.g triton, methanol). Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

Purification: Ascites

Buffer: Ascites with 15mM sodium azide

Research Area: Proliferation Markers, Cell Cycle

Restrictions: For Research Use only

Publications

Yu, Woods, Levison: "The assessment of cellular proliferation by immunohistochemistry: a review of currently available methods and their applications." in: The Histochemical journal, Vol. 24, Issue 3, pp. 121-31, 1992 (PubMed).

Gerdes, Li, Schlueter et al.: "Immunobiochemical and molecular biologic characterization of the cell proliferation-associated nuclear antigen that is defined by monoclonal antibody Ki-67." in: The American journal of pathology, Vol. 138, Issue 4, pp. 867-73, 1991 (PubMed).

Brown, Gatter: "Monoclonal antibody Ki-67: its use in histopathology." in: Histopathology, Vol. 17, Issue 6, pp. 489-503, 1991 (PubMed).

Gerdes: "Ki-67 and other proliferation markers useful for immunohistological diagnostic and prognostic evaluations in human malignancies." in: Seminars in cancer biology, Vol. 1, Issue 3, pp. 199-206, 1991 (PubMed).

Quinn, Wright: "The clinical assessment of proliferation and growth in human tumours: evaluation of methods and applications as prognostic variables." in: The Journal of pathology, Vol. 160, Issue 2, pp. 93-102, 1990 (PubMed).

Schwarting, Gerdes, Niehus et al.: "Determination of the growth fraction in cell suspensions by flow cytometry using the monoclonal antibody Ki-67." in: Journal of immunological methods, Vol. 90, Issue 1, pp. 65-70, 1986 (PubMed).

Gerdes, Schwab, Lemke et al.: "Production of a mouse monoclonal antibody reactive with a human nuclear antigen associated with cell proliferation." in: International journal of cancer. Journal international du cancer, Vol. 31, Issue 1, pp. 13-20, 1983 (PubMed).

Key, Kubbutat, Gerdes: "Assessment of cell proliferation by means of an enzyme-linked immunosorbent assay based on the detection of the Ki-67 protein." in: Journal of immunological methods, Vol. 177, Issue 1-2, pp. 113-7, 1995 (PubMed).