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Antigen Identified By Monoclonal Antibody Ki-67 (MKI67) antibody

Details for Product No. ABIN145923
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Antigen
Synonyms KIA, MIB-1, Ki67, Ki-67, D630048A14Rik, MKI67, kia, mki67, MGC132156
Reactivity
Human
(191), (31), (26), (2), (1), (1), (1)
Host
Mouse
(119), (74), (6), (2)
Clonality
Monoclonal
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB), Immunohistochemistry (IHC)
(92), (68), (56), (54), (31), (25), (13), (10), (9), (1), (1), (1), (1), (1)
Pubmed 8 references available
Quantity 0.1 mL
Shipping to United States (Change)
Catalog No. ABIN145923
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  • +1 404 474 4654
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Immunogen A HeLa cell nuclear preparation.
Isotype IgM
Specificity Monoclonal Anti-Proliferating Cell Protein Ki-67 reacts with the Ki-67 nuclear antigen (345 and 395 kD, and several lower molecular weight proteins) in immuno-blotting. The product shows an immunohistochemical staining pattern similar to that obtained wit
Purification Ascites
Alternative Name Ki-67
Background Ki-67 antigen is the prototypic cell cycle related nuclear protein, expressed by proliferating cells in all phases of the active cell cycle (G1, S, G2 and M phase). It is absent in resting (G0) cells. The monoclonal antibody is useful in establishing the cell growing fraction in neoplasms (immunohistochemically quantified by determining the number of Ki67-positive cells among the total number of resting cells = Ki67 index). In neoplastic tissues the prognostic value is comparable to the tritiated thymidine-labeling index. The correlation between low Ki67 index and histologically low-grade tumors is strong.
Research Area Proliferation Markers, Cell Cycle
Application Notes Recommended dilutions: ICC (IF): 1/200(see review). Fix with 4%PFA. IHC-P: 1/800. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol. IHC-Fr: 1/800 (see review). Fix with formalin. WB: Use at an assay dependant dilution. For immunohisto/cytochemical protocols permeabilisation may be necessary (e.g triton, methanol). Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Restrictions For Research Use only
Format Liquid
Buffer Ascites with 15mM sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
General Yu, Woods, Levison: "The assessment of cellular proliferation by immunohistochemistry: a review of currently available methods and their applications." in: The Histochemical journal, Vol. 24, Issue 3, pp. 121-31, 1992 (PubMed).

Gerdes, Li, Schlueter et al.: "Immunobiochemical and molecular biologic characterization of the cell proliferation-associated nuclear antigen that is defined by monoclonal antibody Ki-67." in: The American journal of pathology, Vol. 138, Issue 4, pp. 867-73, 1991 (PubMed).

Brown, Gatter: "Monoclonal antibody Ki-67: its use in histopathology." in: Histopathology, Vol. 17, Issue 6, pp. 489-503, 1991 (PubMed).

Gerdes: "Ki-67 and other proliferation markers useful for immunohistological diagnostic and prognostic evaluations in human malignancies." in: Seminars in cancer biology, Vol. 1, Issue 3, pp. 199-206, 1991 (PubMed).

Quinn, Wright: "The clinical assessment of proliferation and growth in human tumours: evaluation of methods and applications as prognostic variables." in: The Journal of pathology, Vol. 160, Issue 2, pp. 93-102, 1990 (PubMed).

Schwarting, Gerdes, Niehus et al.: "Determination of the growth fraction in cell suspensions by flow cytometry using the monoclonal antibody Ki-67." in: Journal of immunological methods, Vol. 90, Issue 1, pp. 65-70, 1986 (PubMed).

Gerdes, Schwab, Lemke et al.: "Production of a mouse monoclonal antibody reactive with a human nuclear antigen associated with cell proliferation." in: International journal of cancer. Journal international du cancer, Vol. 31, Issue 1, pp. 13-20, 1983 (PubMed).

Key, Kubbutat, Gerdes: "Assessment of cell proliferation by means of an enzyme-linked immunosorbent assay based on the detection of the Ki-67 protein." in: Journal of immunological methods, Vol. 177, Issue 1-2, pp. 113-7, 1995 (PubMed).

Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Catalog No. ABIN145923
Contact our Customer Service for availability and price in your country.
Add to Basket

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
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