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Antigen Identified By Monoclonal antibody Ki-67 (MKI67) antibody
|Synonyms||KIA, Ki67, Ki-67, D630048A14Rik, MKI67, kia, mki67, MGC132156|
Alternatives Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB), Immunohistochemistry (IHC)
|8 references available|
|Quantity||0.1 ml (Variants)|
|Price||Product not available in this region.|
|Immunogen||A HeLa cell nuclear preparation.|
|Description||Ki-67 antigen is the prototypic cell cycle related nuclear protein, expressed by proliferating cells in all phases of the active cell cycle (G1, S, G2 and M phase). It is absent in resting (G0) cells. The monoclonal antibody is useful in establishing the cell growing fraction in neoplasms (immunohistochemically quantified by determining the number of Ki67-positive cells among the total number of resting cells = Ki67 index). In neoplastic tissues the prognostic value is comparable to the tritiated thymidine-labeling index. The correlation between low Ki67 index and histologically low-grade tumors is strong.|
|Specificity||Monoclonal Anti-Proliferating Cell Protein Ki-67 reacts with the Ki-67 nuclear antigen (345 and 395 kD, and several lower molecular weight proteins) in immuno-blotting. The product shows an immunohistochemical staining pattern similar to that obtained wit|
|Application Notes||Recommended dilutions: ICC (IF): 1/200(see review). Fix with 4%PFA. IHC-P: 1/800. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol. IHC-Fr: 1/800 (see review). Fix with formalin. WB: Use at an assay dependant dilution. For immunohisto/cytochemical protocols permeabilisation may be necessary (e.g triton, methanol). Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user.|
|Buffer||Ascites with 15mM sodium azide|
|Research Area||Proliferation Markers, Cell Cycle|
|Restrictions||For Research Use only|
Yu, Woods, Levison: "The assessment of cellular proliferation by immunohistochemistry: a review of currently available methods and their applications." in: The Histochemical journal, Vol. 24, Issue 3, pp. 121-31, 1992 (PubMed).
Gerdes, Li, Schlueter et al.: "Immunobiochemical and molecular biologic characterization of the cell proliferation-associated nuclear antigen that is defined by monoclonal antibody Ki-67." in: The American journal of pathology, Vol. 138, Issue 4, pp. 867-73, 1991 (PubMed).
Brown, Gatter: "Monoclonal antibody Ki-67: its use in histopathology." in: Histopathology, Vol. 17, Issue 6, pp. 489-503, 1991 (PubMed).
Gerdes: "Ki-67 and other proliferation markers useful for immunohistological diagnostic and prognostic evaluations in human malignancies." in: Seminars in cancer biology, Vol. 1, Issue 3, pp. 199-206, 1991 (PubMed).
Quinn, Wright: "The clinical assessment of proliferation and growth in human tumours: evaluation of methods and applications as prognostic variables." in: The Journal of pathology, Vol. 160, Issue 2, pp. 93-102, 1990 (PubMed).
Schwarting, Gerdes, Niehus et al.: "Determination of the growth fraction in cell suspensions by flow cytometry using the monoclonal antibody Ki-67." in: Journal of immunological methods, Vol. 90, Issue 1, pp. 65-70, 1986 (PubMed).
Gerdes, Schwab, Lemke et al.: "Production of a mouse monoclonal antibody reactive with a human nuclear antigen associated with cell proliferation." in: International journal of cancer. Journal international du cancer, Vol. 31, Issue 1, pp. 13-20, 1983 (PubMed).
Key, Kubbutat, Gerdes: "Assessment of cell proliferation by means of an enzyme-linked immunosorbent assay based on the detection of the Ki-67 protein." in: Journal of immunological methods, Vol. 177, Issue 1-2, pp. 113-7, 1995 (PubMed).
|Hosts||Rabbit (67), Mouse (42), Goat (5)|
|Reactivities||Human (106), Rat (Rattus) (8), Mouse (Murine) (7), Rabbit (1)|
|Applications||Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (57), Western Blotting (WB) (29), ELISA (28), Immunohistochemistry (IHC) (26), Immunofluorescence (IF) (15), Immunohistochemistry (Frozen Sections) (IHC (fro)) (14), Flow Cytometry (FACS) (11), Immunocytochemistry (ICC) (5), Immunohistochemistry (Fixed) (IHC (fx)) (4), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Fluorescence Microscopy (FM) (1)|
|Conjugates||Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), PE,Cy5.5 (1)|
|Epitopes||C-Term (16), Internal Region (4), AA 3118-3256 (2), AA 98-110 (1), N-Term,AA 98-110 (1), Transcript Variant 1 (1)|