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Details for Product No. ABIN145953

Dynein, Axonemal, Intermediate Chain 1 (DNAI1) antibody

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Antigen
Synonyms 1110066F04Rik, BB124644, Dnai1, b2b1526Clo, Dnaic1, CILD1, DIC1, ICS1, PCD
Reactivity
»Alternatives Chicken
Host
»Alternatives Mouse
Clonality Monoclonal
Conjugate
»Alternatives Un-conjugated
Application
»Alternatives Dot Blot (DB), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB), ELISA
Pubmed 6 references available
Catalog no. ABIN145953
Quantity 0.1 mL
Price
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Immunogen Cytoplasmic full length native protein (purified from chick brain) (Chicken).
Isotype IgM
Specificity This antibody identifies the cytoplasmic dynein intermediate chains (triplet of approximately 70 kD) of all vertebrates tested, in immunoblotting with dynein enriched preparations and with crude cell extracts.
Cross-Reactivity Hamster, Human, Rat (Rattus)
Purification Ascites
Alternative Name DNAI1
Background Eukaryotic cells rely on actin and microtubule-based protein "motors" to generate intracellular movements (4). These protein "motors" contain specialized domains that hydrolyze ATP to produce force and movement along a cytoskeletal polymer (actin in the case of myosin family and microtubules in the case of the kinesin family and dyneins). Dynein has been implicated in cytoplasmic motile functions, including chromosomal movement, retrograde organelle and axonal transport, the endocytic pathway, and the organization of the Golgi apparatus. In all cell types, dynein has the same basic structures and is composed of two or three distinct heavy chains (~450 kD), three intermediate chains (70-125 kD), and at least four light chains (15-25 kD) (5).
Application Notes Dot: Use at an assay dependant dilution. ELISA: Use at an assay dependant dilution. FuncS: Use at an assay dependant dilution. ICC: Use at an assay dependant dilution. IF: Use at a dilution of 1/100-1/200. IHC-P: Use at a dilution of 1/200 (see ref Dombeck DA et al). Antigen retrieval is not essential but may optimise staining. Inhib: Use at an assay dependant dilution. P: Use at an assay dependant dilution. WB: Use at a dilution of 1/2000. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Restrictions For Research Use only
Format Liquid
Concentration Ascites Fluid
Buffer Ascitic fluid with 0.1% sodium azide
Preservative Sodium azide
Storage -20 °C
Zinkowski, Meyne, Brinkley: "The centromere-kinetochore complex: a repeat subunit model." in: The Journal of cell biology, Vol. 113, Issue 5, pp. 1091-110, 1991 (PubMed).

Wordeman, Steuer, Sheetz et al.: "Chemical subdomains within the kinetochore domain of isolated CHO mitotic chromosomes." in: The Journal of cell biology, Vol. 114, Issue 2, pp. 285-94, 1991 (PubMed).

Mocz, Farias, Gibbons: "Proteolytic analysis of domain structure in the beta heavy chain of dynein from sea urchin sperm flagella." in: Biochemistry, Vol. 30, Issue 29, pp. 7225-31, 1991 (PubMed).

Steuer, Wordeman, Schroer et al.: "Localization of cytoplasmic dynein to mitotic spindles and kinetochores." in: Nature, Vol. 345, Issue 6272, pp. 266-8, 1990 (PubMed).

Vallee, Shpetner: "Motor proteins of cytoplasmic microtubules." in: Annual review of biochemistry, Vol. 59, pp. 909-32, 1990 (PubMed).

Nilsson, Wallin: "Evidence for several roles of dynein in pigment transport in melanophores." in: Cell motility and the cytoskeleton, Vol. 38, Issue 4, pp. 397-409, 1998 (PubMed).

Alternatives for antigen "Dynein, Axonemal, Intermediate Chain 1 (DNAI1)", type "Antibodies"
Hosts (25), (4)
Reactivities (29), (18), (12), (6)
Applications (19), (12), (10), (3), (3), (3), (1)
Conjugates (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (4), (4), (2), (2), (1)
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