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Dynein, Axonemal, Intermediate Chain 1 (DNAI1) antibody

Details for Product No. ABIN145953, Supplier: Login to see
Antigen
  • CILD1
  • DIC1
  • ICS1
  • PCD
  • 1110066F04Rik
  • BB124644
  • Dnai1
  • b2b1526Clo
  • Dnaic1
Reactivity
Chicken
43
9
7
2
Host
Mouse
35
8
Clonality
Monoclonal
Conjugate
Un-conjugated
2
2
2
2
2
2
Application
Dot Blot (DB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Purification (Purif), ELISA, Western Blotting (WB)
38
26
1
1
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Immunogen Cytoplasmic full length native protein (purified from chick brain) (Chicken).
Isotype IgM
Specificity This antibody identifies the cytoplasmic dynein intermediate chains (triplet of approximately 70 kD) of all vertebrates tested, in immunoblotting with dynein enriched preparations and with crude cell extracts.
Cross-Reactivity Human, Hamster, Muntiacus muntjak, Rat (Rattus)
Characteristics Cellular Localization: Cytoplasmic
Purification Ascites.
Alternative Name DNAI1 (DNAI1 Antibody Abstract)
Background Eukaryotic cells rely on actin and microtubule-based protein ""motors"" to generate intracellular movements (4). These protein ""motors"" contain specialized domains that hydrolyze ATP to produce force and movement along a cytoskeletal polymer (actin in the case of myosin family and microtubules in the case of the kinesin family and dyneins). Dynein has been implicated in cytoplasmic motile functions, including chromosomal movement, retrograde organelle and axonal transport, the endocytic pathway, and the organization of the Golgi apparatus. In all cell types, dynein has the same basic structures and is composed of two or three distinct heavy chains (~450 kD), three intermediate chains (70-125 kD), and at least four light chains (15-25 kD) (5).
Synonyms: DNAI1, ICS, MGC26204, 242650, Dynein Intermediate chain, PCD, Q9UI46, CILD1, 27019
Pathways
Application Notes Dot: Use at an assay dependant dilution. ELISA: Use at an assay dependant dilution. FuncS: Use at an assay dependant dilution. ICC: Use at an assay dependant dilution. IF: Use at a dilution of 1/100-1/200. IHC-P: Use at a dilution of 1/200 (see ref Dombeck DA et al). Antigen retrieval is not essential but may optimise staining. Inhib: Use at an assay dependant dilution. P: Use at an assay dependant dilution. WB: Use at a dilution of 1/2000. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Restrictions For Research Use only
Format Liquid
Buffer Ascitic fluid containing 15 mM sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles.
Storage -20 °C
Storage Comment Upon receipt - Keep as concentrated solution. Aliquot and store at -20°C or below.
Background publications Nilsson, Wallin: "Evidence for several roles of dynein in pigment transport in melanophores." in: Cell motility and the cytoskeleton, Vol. 38, Issue 4, pp. 397-409, 1998 (PubMed).

Vallee, Shpetner: "Motor proteins of cytoplasmic microtubules." in: Annual review of biochemistry, Vol. 59, pp. 909-32, 1990 (PubMed).

Steuer, Wordeman, Schroer et al.: "Localization of cytoplasmic dynein to mitotic spindles and kinetochores." in: Nature, Vol. 345, Issue 6272, pp. 266-8, 1990 (PubMed).

Mocz, Farias, Gibbons: "Proteolytic analysis of domain structure in the beta heavy chain of dynein from sea urchin sperm flagella." in: Biochemistry, Vol. 30, Issue 29, pp. 7225-31, 1991 (PubMed).

Wordeman, Steuer, Sheetz et al.: "Chemical subdomains within the kinetochore domain of isolated CHO mitotic chromosomes." in: The Journal of cell biology, Vol. 114, Issue 2, pp. 285-94, 1991 (PubMed).

Zinkowski, Meyne, Brinkley: "The centromere-kinetochore complex: a repeat subunit model." in: The Journal of cell biology, Vol. 113, Issue 5, pp. 1091-110, 1991 (PubMed).