Dynein, Axonemal, Intermediate Chain 1 (DNAI1) antibody

Antigen: Dynein, Axonemal, Intermediate Chain 1 (DNAI1)

Synonyms: Dnai1, BB124644, 1110066F04Rik, Dnaic1, MGC133918

Clonality: Monoclonal

Host: Mouse

Reactivity: Chicken

Conjugate: Un-conjugated

Application: Dot Blot (Dot), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB), ELISA

Catalog no.: ABIN145953

Additional Information

Alternative name: DNAI1

Immunogen: Cytoplasmic full length native protein (purified from chick brain) (Chicken).

Cross-Reactivity: Chicken, Hamster, Human, Rat (Rattus)

Isotype: IgM

Description: """ Eukaryotic cells rely on actin and microtubule-based protein """"""""""""""""motors"""""""""""""""" to generate intracellular movements (4). These protein """"""""""""""""motors"""""""""""""""" contain specialized domains that hydrolyze ATP to produce force and movement along a cytoskeletal polymer (actin in the case of myosin family and microtubules in the case of the kinesin family and dyneins). Dynein has been implicated in cytoplasmic motile functions, including chromosomal movement, retrograde organelle and axonal transport, the endocytic pathway, and the organization of the Golgi apparatus. In all cell types, dynein has the same basic structures and is composed of two or three distinct heavy chains (~450 kD), three intermediate chains (70-125 kD), and at least four light chains (15-25 kD) (5)."""""""""

Specificity: This antibody identifies the cytoplasmic dynein intermediate chains (triplet of approximately 70 kD) of all vertebrates tested, in immunoblotting with dynein enriched preparations and with crude cell extracts.

Application Details

Application Notes: Dot: Use at an assay dependant dilution. ELISA: Use at an assay dependant dilution. FuncS: Use at an assay dependant dilution. ICC: Use at an assay dependant dilution. IF: Use at a dilution of 1/100-1/200. IHC-P: Use at a dilution of 1/200 (see ref Dombeck DA et al). Antigen retrieval is not essential but may optimise staining. Inhib: Use at an assay dependant dilution. P: Use at an assay dependant dilution. WB: Use at a dilution of 1/2000. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

Concentration: Ascites Fluid

Purification: Ascites

Buffer: Ascitic fluid with 0.1% sodium azide

Storage: Aliquot and store at -20 C. Avoid repeated freezing and thawing. Storage in frost-free freezers is not recommended.

Restrictions: For Research Use only

Publications

Zinkowski, Meyne, Brinkley: "The centromere-kinetochore complex: a repeat subunit model." in: The Journal of cell biology, Vol. 113, Issue 5, pp. 1091-110, 1991 (PubMed).

Wordeman, Steuer, Sheetz et al.: "Chemical subdomains within the kinetochore domain of isolated CHO mitotic chromosomes." in: The Journal of cell biology, Vol. 114, Issue 2, pp. 285-94, 1991 (PubMed).

Mocz, Farias, Gibbons: "Proteolytic analysis of domain structure in the beta heavy chain of dynein from sea urchin sperm flagella." in: Biochemistry, Vol. 30, Issue 29, pp. 7225-31, 1991 (PubMed).

Steuer, Wordeman, Schroer et al.: "Localization of cytoplasmic dynein to mitotic spindles and kinetochores." in: Nature, Vol. 345, Issue 6272, pp. 266-8, 1990 (PubMed).

Vallee, Shpetner: "Motor proteins of cytoplasmic microtubules." in: Annual review of biochemistry, Vol. 59, pp. 909-32, 1990 (PubMed).

Nilsson, Wallin: "Evidence for several roles of dynein in pigment transport in melanophores." in: Cell motility and the cytoskeleton, Vol. 38, Issue 4, pp. 397-409, 1998 (PubMed).