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beta Actin antibody (Actin, beta) (AA 1-14)

Details for Product anti-ACTB Antibody No. ABIN145954, Supplier: Login to see
Antigen
  • beta-actin
  • ps1tp5bp1
  • LOC563571
  • ACTB
  • A26C1A
  • A26C1B
  • act-2
  • actb
  • B-actin
  • LOC100136013
  • LOC100174883
  • LOC100304816
  • A
  • ACT5C
  • Ac5C
  • Act
  • Act-5C
  • Act5
  • Act5c
  • Actin
  • Actin/BAP47
  • Actin5C
  • BAP47
  • Bap47
  • CG4027
  • Dmel\\CG4027
  • M32055
  • T11
  • act
  • act 5C
  • act5C
  • actin
  • actin5C
  • anon-EST:fe2D2
  • beta-actin/Bap47
  • cyt5C
  • l(1)G0009
  • l(1)G0010
  • l(1)G0025
  • l(1)G0079
  • l(1)G0117
  • l(1)G0177
  • l(1)G0245
  • l(1)G0330
  • l(1)G0420
  • l(1)G0486
  • Bact
  • BRWS1
  • PS1TP5BP1
  • ACT-5
  • Actx
  • E430023M04Rik
  • ACTA1
  • Beta-actin
  • B-ACTZF
  • actba
  • bact
  • bactin1
  • bactzf
  • wu:fd18f05
  • POTE2alpha
  • POTEACTIN
Epitope
AA 1-14
123
38
15
14
11
7
6
4
4
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Xenopus laevis
474
189
166
49
48
39
35
31
29
22
18
14
11
8
8
7
6
5
4
4
4
3
2
2
2
2
2
1
1
1
1
1
1
1
1
Host
Mouse
307
197
19
1
1
Clonality (Clone)
Monoclonal ()
Conjugate
This beta Actin antibody is un-conjugated
26
19
15
4
4
4
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Dot Blot (DB), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunomicroscopy (IM), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Western Blotting (WB)
453
209
147
84
80
47
37
21
12
11
11
11
8
3
2
2
2
2
1
1
1
Supplier
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Immunogen Synthetic peptide: DDDIAALVIDNGSGL conjugated to KLH, corresponding to amino acids 1-14 of Xenopus laevis Actin (beta).
Clone AC-15
Isotype IgG1
Specificity This is an ideal reagent for use as a cell extract loading control on Western blot, Molecular weight of beta actin is 42kD.
Cross-Reactivity Human, Mouse (Murine), Carp, Cat (Feline), Cow (Bovine), Dog (Canine), Fruit Fly (Drosophila melanogaster), Fish, Guinea Pig, Pig (Porcine), Rabbit, Rat (Rattus), Sheep (Ovine)
Characteristics Cellular Localization: Cytoplasmic
Purification Unpurified. Ascites
Alternative Name Actin beta / ACTB (ACTB Antibody Abstract)
Background Beta actin is one of six different actin isoforms which have been identified. Actins are highly conserved proteins that are involved in cell motility, structure and integrity.
Synonyms: ACTB, beta Actin, P60709, 102630, 60
Pathways
Application Notes AF: Use at an assay dependant dilution. Dot: Use at an assay dependant dilution. EC: Use at an assay dependant dilution. IHC-F: Use at an assay dependant dilution. ICC: Use at a dilution of 1/1000. IF: Use at an assay dependant dilution. IHC-P: Use at an assay dependant dilution. The epitope recognised by the antibody is resistant to formalin fixation and paraffin embedding. B5, methacarn, ethanol or Bouins solutions may also be used as fixatives. IHC-Fr: Use at an assay dependant dilution. IM: Use at an assay dependant dilution. I-ELISA: Use at an assay dependant dilution. WB: Use at a dilution of 1/1000-1/5000 Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Comment

Positive Controls: Cultured human or chicken fibroblasts as described in Liao et alMolecular weight of beta actin is 42Kd

Restrictions For Research Use only
Format Liquid
Concentration 2 mg/ml
Buffer Ascitic fluid containing 15mM sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles.
Storage 4 °C
Storage Comment Keep as concentrated solution. Store at 4°C short term. For extended storage aliquot and store at -20°C or below.
Supplier Images
 image for anti-beta Actin antibody (Actin, beta) (AA 1-14) (ABIN145954) anti-Actin, beta (ACTB) (AA 1-14) antibody
Background publications Hoemme, Schmitt, Himmele et al.: "Vitamin D and dexamethasone inversely regulate parathyroid hormone-induced regulator of G protein signaling-2 expression in osteoblast-like cells." in: Endocrinology, Vol. 144, Issue 6, pp. 2496-504, 2003 (PubMed).

Ikeyama, Kokkonen, Martindale et al.: "Effects of aging and calorie restriction of Fischer 344 rats on hepatocellular response to proliferative signals." in: Experimental gerontology, Vol. 38, Issue 4, pp. 431-9, 2003 (PubMed).

Lichtenfels, Kellner, Atkins et al.: "Identification of metabolic enzymes in renal cell carcinoma utilizing PROTEOMEX analyses." in: Biochimica et biophysica acta, Vol. 1646, Issue 1-2, pp. 21-31, 2003 (PubMed).

Bruce, Beckmann, Ribeiro et al.: "A band 3-based macrocomplex of integral and peripheral proteins in the RBC membrane." in: Blood, Vol. 101, Issue 10, pp. 4180-8, 2003 (PubMed).

Helmlinger, Yvert, Picaud et al.: "Progressive retinal degeneration and dysfunction in R6 Huntington's disease mice." in: Human molecular genetics, Vol. 11, Issue 26, pp. 3351-9, 2002 (PubMed).

Counts, McGuire, Sortwell et al.: "Galanin inhibits tyrosine hydroxylase expression in midbrain dopaminergic neurons." in: Journal of neurochemistry, Vol. 83, Issue 2, pp. 442-51, 2002 (PubMed).

Gao, Hwang, Bataille et al.: "Serine phosphorylation of insulin receptor substrate 1 by inhibitor kappa B kinase complex." in: The Journal of biological chemistry, Vol. 277, Issue 50, pp. 48115-21, 2002 (PubMed).

Ueda, Mawji, Bruchovsky et al.: "Ligand-independent activation of the androgen receptor by interleukin-6 and the role of steroid receptor coactivator-1 in prostate cancer cells." in: The Journal of biological chemistry, Vol. 277, Issue 41, pp. 38087-94, 2002 (PubMed).

Mohajeri, Wollmer, Nitsch: "Abeta 42-induced increase in neprilysin is associated with prevention of amyloid plaque formation in vivo." in: The Journal of biological chemistry, Vol. 277, Issue 38, pp. 35460-5, 2002 (PubMed).

Hahn, Dessain, Brooks et al.: "Enumeration of the simian virus 40 early region elements necessary for human cell transformation." in: Molecular and cellular biology, Vol. 22, Issue 7, pp. 2111-23, 2002 (PubMed).

Vaziri, Dessain, Ng Eaton et al.: "hSIR2(SIRT1) functions as an NAD-dependent p53 deacetylase." in: Cell, Vol. 107, Issue 2, pp. 149-59, 2001 (PubMed).

Liao, Xu, Wargovich: "Direct reprobing with anti-beta-actin antibody as an internal control for western blotting analysis." in: BioTechniques, Vol. 28, Issue 2, pp. 216-8, 2000 (PubMed).

Gimona, Vandekerckhove, Goethals et al.: "Beta-actin specific monoclonal antibody." in: Cell motility and the cytoskeleton, Vol. 27, Issue 2, pp. 108-16, 1994 (PubMed).

North, Gimona, Lando et al.: "Actin isoform compartments in chicken gizzard smooth muscle cells." in: Journal of cell science, Vol. 107 ( Pt 3), pp. 445-55, 1994 (PubMed).

North, Gimona, Cross et al.: "Calponin is localised in both the contractile apparatus and the cytoskeleton of smooth muscle cells." in: Journal of cell science, Vol. 107 ( Pt 3), pp. 437-44, 1994 (PubMed).