Actin, beta (ACTB) antibody

Antigen: Actin, beta (ACTB)

Synonyms: PS1TP5BP1, Actx, beta-actin, E430023M04Rik, ACTB, MGC128179, MGC76228, MGC79692, MGC88999, ps1tp5bp1, LOC442937, LOC563571, A26C1A, A26C1B, ACT-5, act-2, Actb, actb, B-actin, LOC100136013, DKFZp459A196, LOC100174883, LOC100304816, ACTA1

Clonality: Monoclonal

Host: Mouse

Reactivity: Cow (Bovine)

Conjugate: Un-conjugated

Application: Dot Blot (Dot), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB), Immunoelectron Microscopy (IEM), Immunohistochemistry (IHC), ELISA

Catalog no.: ABIN145954

Additional Information

Alternative name: Actin beta / ACTB

Immunogen: Synthetic peptide: DDDIAALVIDNGSGL conjugated to KLH, corresponding to amino acids 1-14 of Xenopus laevis Actin (beta).

Cross-Reactivity: Cow (Bovine), Dog (Canine), Chicken, Cat (Feline), Guinea Pig, Human, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus), Sheep (Ovine)

Format: Liquid

Isotype: IgG1

Description: Beta actin is one of six different actin isoforms which have been identified. Actins are highly conserved proteins that are involved in cell motility, structure and integrity.

Specificity: We no longer recommend this antibody for use as a loading control, we instead recommend GTX28226 as an alternative. GTX28226 has been produced specifically for use as a loading control.

Synonyms: PS1TP5BP1, Actx, beta-actin, E430023M04Rik, ACTB, MGC128179, MGC76228, MGC79692, MGC88999, ps1tp5bp1, LOC442937, LOC563571, A26C1A, A26C1B, ACT-5, act-2, Actb, actb, B-actin, LOC100136013, DKFZp459A196, LOC100174883, LOC100304816, ACTA1

Application Details

Application Notes: AF: Use at an assay dependant dilution. Dot: Use at an assay dependant dilution. EC: Use at an assay dependant dilution. IHC-F: Use at an assay dependant dilution. ICC: Use at a dilution of 1/1000. IF: Use at an assay dependant dilution. IHC-P: Use at an assay dependant dilution. The epitope recognised by the antibody is resistant to formalin fixation and paraffin embedding. B5, methacarn, ethanol or Bouins solutions may also be used as fixatives. IHC-Fr: Use at an assay dependant dilution. IM: Use at an assay dependant dilution. I-ELISA: Use at an assay dependant dilution. WB: Use at a dilution of 1/1000-1/5000 Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

Concentration: 2 mg/ml

Purification: Ascites

Buffer: Ascitic fluid with 15mM sodium azide

Storage: Store at 4 C. Aliquot and store at -20 C long-term. Avoid multiple freeze/thaw.

Restrictions: For Research Use only

Images

anti-Actin, beta (ACTB) antibody

Publications

North, Gimona, Cross et al.: "Calponin is localised in both the contractile apparatus and the cytoskeleton of smooth muscle cells." in: Journal of cell science, Vol. 107 ( Pt 3), pp. 437-44, 1994 (PubMed).

North, Gimona, Lando et al.: "Actin isoform compartments in chicken gizzard smooth muscle cells." in: Journal of cell science, Vol. 107 ( Pt 3), pp. 445-55, 1994 (PubMed).

Gimona, Vandekerckhove, Goethals et al.: "Beta-actin specific monoclonal antibody." in: Cell motility and the cytoskeleton, Vol. 27, Issue 2, pp. 108-16, 1994 (PubMed).

Liao, Xu, Wargovich: "Direct reprobing with anti-beta-actin antibody as an internal control for western blotting analysis." in: BioTechniques, Vol. 28, Issue 2, pp. 216-8, 2000 (PubMed).

Vaziri, Dessain, Ng Eaton et al.: "hSIR2(SIRT1) functions as an NAD-dependent p53 deacetylase." in: Cell, Vol. 107, Issue 2, pp. 149-59, 2001 (PubMed).

Hahn, Dessain, Brooks et al.: "Enumeration of the simian virus 40 early region elements necessary for human cell transformation." in: Molecular and cellular biology, Vol. 22, Issue 7, pp. 2111-23, 2002 (PubMed).

Mohajeri, Wollmer, Nitsch: "Abeta 42-induced increase in neprilysin is associated with prevention of amyloid plaque formation in vivo." in: The Journal of biological chemistry, Vol. 277, Issue 38, pp. 35460-5, 2002 (PubMed).

Ueda, Mawji, Bruchovsky et al.: "Ligand-independent activation of the androgen receptor by interleukin-6 and the role of steroid receptor coactivator-1 in prostate cancer cells." in: The Journal of biological chemistry, Vol. 277, Issue 41, pp. 38087-94, 2002 (PubMed).

Gao, Hwang, Bataille et al.: "Serine phosphorylation of insulin receptor substrate 1 by inhibitor kappa B kinase complex." in: The Journal of biological chemistry, Vol. 277, Issue 50, pp. 48115-21, 2002 (PubMed).

Counts, McGuire, Sortwell et al.: "Galanin inhibits tyrosine hydroxylase expression in midbrain dopaminergic neurons." in: Journal of neurochemistry, Vol. 83, Issue 2, pp. 442-51, 2002 (PubMed).

Helmlinger, Yvert, Picaud et al.: "Progressive retinal degeneration and dysfunction in R6 Huntington's disease mice." in: Human molecular genetics, Vol. 11, Issue 26, pp. 3351-9, 2002 (PubMed).

Bruce, Beckmann, Ribeiro et al.: "A band 3-based macrocomplex of integral and peripheral proteins in the RBC membrane." in: Blood, Vol. 101, Issue 10, pp. 4180-8, 2003 (PubMed).

Lichtenfels, Kellner, Atkins et al.: "Identification of metabolic enzymes in renal cell carcinoma utilizing PROTEOMEX analyses." in: Biochimica et biophysica acta, Vol. 1646, Issue 1-2, pp. 21-31, 2003 (PubMed).

Ikeyama, Kokkonen, Martindale et al.: "Effects of aging and calorie restriction of Fischer 344 rats on hepatocellular response to proliferative signals." in: Experimental gerontology, Vol. 38, Issue 4, pp. 431-9, 2003 (PubMed).

Hoemme, Schmitt, Himmele et al.: "Vitamin D and dexamethasone inversely regulate parathyroid hormone-induced regulator of G protein signaling-2 expression in osteoblast-like cells." in: Endocrinology, Vol. 144, Issue 6, pp. 2496-504, 2003 (PubMed).