Dynein, Axonemal, Heavy Chain 9 (DNAH9) (Cytoplasmic Domain) antibody

Details for Product No. ABIN145956
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Antigen
Synonyms Bv2, DNAH17L, DNEL1, DYH9, Dnahc9, HL-20, HL20, 9030022M04, A230091C01, C230051G16, D11Ertd686e, mKIAA0357
Epitope
Cytoplasmic Domain
(2)
Reactivity
Chicken
(14), (12), (12), (12)
Host
Mouse
(14)
Clonality
Monoclonal
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB)
(10), (3), (2), (1)
Pubmed 4 references available
Quantity 0.1 mL
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Catalog No. ABIN145956
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Immunogen Cytoplasmic full length native protein (purified from chick brain) (Chicken).
Isotype IgG2a
Specificity The antibody identifies the cytoplasmic dynein heavy chain (440 kDa) in immunoblotting with dynein enriched preparations and in crude cell extracts of chicken brain and fibroblasts.
Cross-Reactivity Human
Characteristics Cellular Localization: Cytoplasmic. With intact mitotic chicken embryo fibroblasts, the labeling of the spindle with the antibody is clearly visible with diffuse cytoplasmic staining by immunofluorescence techniques (Wordeman L et al.).
Purification Ascites.
Alternative Name DNAH9
Background Eukaryotic cells rely on actin and microtubule-based protein ""motors"" to generate intracellular movements (3). These protein ""motors"" contain specialized domains that hydrolyze ATP to produce force and movement along a cytoskeletal polymer (actin in the case of myosin family and microtubules in the case of the kinesin family and dyneins). Dynein has been implicated in cytoplasmic motile functions, including chromosomal movement, retrograde organelle and axonal transport, the endocytic pathway, and the organization of the Golgi apparatus. In all cell types, dynein has the same basic structures and is composed of two or three distinct heavy chains (~450 kD), three intermediate chains (70-125 kD), and at least four light chains (15-25 kD) (4).
Synonyms: HL20, Dynein Heavy chain, DNAH9, HL-20, Dnahc9, KIAA0357, Q9NYC9, DNAH17L, 603330, DNEL1, DYH9, 1770, HL 20
Application Notes ELISA: Use at an assay dependant dilution. IF: Use at an assay dependant dilution. WB (colorimetric): Use at a dilution of 1/100 for chick brain extract. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Restrictions For Research Use only
Format Liquid
Buffer Ascitic fluid with 0.1% sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C long-term.
General Wordeman, Steuer, Sheetz et al.: "Chemical subdomains within the kinetochore domain of isolated CHO mitotic chromosomes." in: The Journal of cell biology, Vol. 114, Issue 2, pp. 285-94, 1991 (PubMed).

Mocz, Farias, Gibbons: "Proteolytic analysis of domain structure in the beta heavy chain of dynein from sea urchin sperm flagella." in: Biochemistry, Vol. 30, Issue 29, pp. 7225-31, 1991 (PubMed).

Steuer, Wordeman, Schroer et al.: "Localization of cytoplasmic dynein to mitotic spindles and kinetochores." in: Nature, Vol. 345, Issue 6272, pp. 266-8, 1990 (PubMed).

Vallee, Shpetner: "Motor proteins of cytoplasmic microtubules." in: Annual review of biochemistry, Vol. 59, pp. 909-32, 1990 (PubMed).

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