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Collagen, Type IV (COL4) antibody

Details for Product No. ABIN145959
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Antigen
Synonyms
1209, 6072, CG16858, CT25584, Coll IValpha2, DmColA2, Dmel\\CG16858, Vkg, VkgC, alpha(IV)2/vkg, col4a2, coll. IV, l(2)01209, CG25C, CG4145, CT12803, Cgc25, Cgc25C, Coll, Coll IV, DCg1, Dcg, Dcg1, Dmel ... show more
1209, 6072, CG16858, CT25584, Coll IValpha2, DmColA2, Dmel\\CG16858, Vkg, VkgC, alpha(IV)2/vkg, col4a2, coll. IV, l(2)01209, CG25C, CG4145, CT12803, Cgc25, Cgc25C, Coll, Coll IV, DCg1, Dcg, Dcg1, Dmel\\CG4145, alpha(IV)1/Cg25C, alpha1, cg25C, cg25c, col4a1, gp125 show less
Reactivity
Human
(174), (53), (44), (35), (33), (25), (12), (4), (1), (1), (1)
Host
Mouse
(110), (76), (17), (1)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(17), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Application
Dot Blot (DB), ELISA, Immunoassay (IA), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(128), (105), (95), (74), (52), (31), (30), (26), (20), (17), (12), (2), (2), (2), (1)
Pubmed 3 references available
Quantity 0.1 mL
Shipping to United States (Change)
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Catalog No. ABIN145959
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Immunogen Full length native protein (purified) (Human).
Clone COL-94
Isotype IgG1
Specificity Specifically recognizes native, undenatured, human collagen type IV . Shows no cross reactivity with collagen types I, II, III, V, VI, VII, human vitronectin, fibronectin, or chondroitin sulfate A. B and C. No reactivity with denatured or denatured-reduced collagen in immunoblots.
Cross-Reactivity Primate
Characteristics Cellular Localization: Cell surface (Potential).
Purification Ascites.
Alternative Name Collagen type IV
Background Collagen IV is a major constituent of the basement membranes along with laminins and enactins. It is composed of alpha 1 IV chain and alpha 2 IV chain in 2:1 ratio . It can form insoluble fibers with high tensile strength. Antibody to collagen IV is useful in detecting the loss of parts of basement membrane in carcinomas.
Synonyms: 120070, FLJ22259, MGC42377, 1285, P02462, 1284, ATS, 1286, COL4A5, 120130, P08572, COL4A4, 1287, CA54, Collagen IV, COL4A2, CA44, 120131, Q01955, COL4A3, 301050, 120090, 1282, ASLN, COL4A1, P29400, P53420, arresten
Research Area Extracellular Matrix
Application Notes Dot: Use at an assay dependant dilution. ELISA: Use at an assay dependant dilution. IHC-F: Use at a dilution of 1/500. ICC: Use at an assay dependant dilution. IHC-P: Use at an assay dependant dilution. WB: Use at an assay dependant dilution. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Comment

Positive Controls: Human tissue

Restrictions For Research Use only
Format Liquid
Buffer Preservative: 15mM Sodium Azide, Constituents: Ascites fluid
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles.
Storage 4 °C
Storage Comment Keep as concentrated solution. Store at 4°C short term. For extended storage aliquot and store at -20°C or below.
General Sage, Woodbury, Bornstein: "Structural studies on human type IV collagen." in: The Journal of biological chemistry, Vol. 254, Issue 19, pp. 9893-900, 1979 (PubMed).

Sanes, Engvall, Butkowski et al.: "Molecular heterogeneity of basal laminae: isoforms of laminin and collagen IV at the neuromuscular junction and elsewhere." in: The Journal of cell biology, Vol. 111, Issue 4, pp. 1685-99, 1990 (PubMed).

Gay, Fine: "Characterization and isolation of poly- and monoclonal antibodies against collagen for use in immunohistochemistry." in: Methods in enzymology, Vol. 145, pp. 148-67, 1987 (PubMed).

Validation Images
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