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Details for Product No. ABIN145959

Collagen, Type IV (COL4) antibody

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Antigen
Synonyms
1209, 6072, CG16858, CT25584, Coll IValpha2, DmColA2, Dmel\\CG16858, Vkg, VkgC, alpha(IV)2/vkg, col4a2, coll. IV, l(2)01209, CG25C, CG4145, CT12803, Cgc25, Cgc25C, Coll, Coll IV, DCg1, Dcg, Dcg1, Dmel ... show more
Reactivity
»Alternatives Human
Host
»Alternatives Mouse
Clonality Monoclonal
Conjugate
»Alternatives Un-conjugated
Application
»Alternatives Dot Blot (DB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB), Immunohistochemistry (IHC), ELISA
Pubmed 3 references available
Catalog no. ABIN145959
Quantity 0.1 mL
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Immunogen Full length native protein (purified) (Human).
Isotype IgG1
Specificity Specifically recognizes native, undenatured, human collagen type IV . Shows no cross reactivity with collagen types I, II, III, V, VI, VII, human vitronectin, fibronectin, or chondroitin sulfate A. B and C. No reactivity with denatured or denatured-reduce
Cross-Reactivity Monkey
Purification Ascites
Alternative Name Collagen type IV
Background Collagen IV is a major constituent of the basement membranes along with laminins and enactins. It is composed of alpha 1 IV chain and alpha 2 IV chain in 2:1 ratio . It can form insoluble fibers with high tensile strength. Antibody to collagen IV is useful in detecting the loss of parts of basement membrane in carcinomas.
Research Area Extracellular Matrix
Application Notes Dot: Use at an assay dependant dilution. ELISA: Use at an assay dependant dilution. IHC-F: Use at a dilution of 1/500. ICC: Use at an assay dependant dilution. IHC-P: Use at an assay dependant dilution. WB: Use at an assay dependant dilution. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Restrictions For Research Use only
Format Liquid
Buffer Ascites fluid
Preservative Sodium azide
Sage, Woodbury, Bornstein: "Structural studies on human type IV collagen." in: The Journal of biological chemistry, Vol. 254, Issue 19, pp. 9893-900, 1979 (PubMed).

Sanes, Engvall, Butkowski et al.: "Molecular heterogeneity of basal laminae: isoforms of laminin and collagen IV at the neuromuscular junction and elsewhere." in: The Journal of cell biology, Vol. 111, Issue 4, pp. 1685-99, 1990 (PubMed).

Gay, Fine: "Characterization and isolation of poly- and monoclonal antibodies against collagen for use in immunohistochemistry." in: Methods in enzymology, Vol. 145, pp. 148-67, 1987 (PubMed).

Alternatives for antigen "Collagen, Type IV (COL4)", type "Antibodies"
Hosts (110), (90), (19), (1)
Reactivities (189), (51), (50), (39), (35), (26), (12), (5), (2), (2), (2), (1)
Applications (142), (118), (74), (68), (55), (35), (32), (26), (26), (20), (20), (15), (5), (2), (1), (1)
Conjugates (19), (5), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Epitopes (1), (1), (1), (1)
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