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Collagen IV antibody (Collagen, Type IV)

Details for Product anti-COL4 Antibody No. ABIN145959, Supplier: Login to see
Antigen
  • 1209
  • 6072
  • CG16858
  • CT25584
  • Coll IValpha2
  • DmColA2
  • Dmel\\CG16858
  • Vkg
  • VkgC
  • alpha(IV)2/vkg
  • col4a2
  • coll. IV
  • l(2)01209
  • CG25C
  • CG4145
  • CT12803
  • Cgc25
  • Cgc25C
  • Coll
  • Coll IV
  • DCg1
  • Dcg
  • Dcg1
  • Dmel\\CG4145
  • alpha(IV)1/Cg25C
  • alpha1
  • cg25C
  • cg25c
  • col4a1
  • gp125
Reactivity
Human
224
54
38
23
11
4
2
2
1
1
1
Host
Mouse
132
98
29
1
Clonality (Clone)
Monoclonal ()
Conjugate
This Collagen IV antibody is un-conjugated
19
8
5
3
3
3
2
2
2
2
2
2
2
1
1
1
Application
Dot Blot (DB), Immunoassay (IA), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Western Blotting (WB)
131
106
95
86
73
54
23
23
22
22
21
15
5
2
2
2
1
1
1
1
Supplier
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Immunogen Full length native protein (purified) (Human).
Clone COL-94
Isotype IgG1
Specificity Specifically recognizes native, undenatured, human collagen type IV . Shows no cross reactivity with collagen types I, II, III, V, VI, VII, human vitronectin, fibronectin, or chondroitin sulfate A. B and C. No reactivity with denatured or denatured-reduced collagen in immunoblots.
Cross-Reactivity Primate
Characteristics Cellular Localization: Cell surface (Potential).
Purification Ascites.
Alternative Name Collagen type IV (COL4 Antibody Abstract)
Background Collagen IV is a major constituent of the basement membranes along with laminins and enactins. It is composed of alpha 1 IV chain and alpha 2 IV chain in 2:1 ratio . It can form insoluble fibers with high tensile strength. Antibody to collagen IV is useful in detecting the loss of parts of basement membrane in carcinomas.
Synonyms: 120070, FLJ22259, MGC42377, 1285, P02462, 1284, ATS, 1286, COL4A5, 120130, P08572, COL4A4, 1287, CA54, Collagen IV, COL4A2, CA44, 120131, Q01955, COL4A3, 301050, 120090, 1282, ASLN, COL4A1, P29400, P53420, arresten
Research Area Extracellular Matrix
Pathways
Application Notes Dot: Use at an assay dependant dilution. ELISA: Use at an assay dependant dilution. IHC-F: Use at a dilution of 1/500. ICC: Use at an assay dependant dilution. IHC-P: Use at an assay dependant dilution. WB: Use at an assay dependant dilution. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Comment

Positive Controls: Human tissue

Restrictions For Research Use only
Format Liquid
Buffer Preservative: 15mM Sodium Azide, Constituents: Ascites fluid
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles.
Storage 4 °C
Storage Comment Keep as concentrated solution. Store at 4°C short term. For extended storage aliquot and store at -20°C or below.
Background publications Gay, Fine: "Characterization and isolation of poly- and monoclonal antibodies against collagen for use in immunohistochemistry." in: Methods in enzymology, Vol. 145, pp. 148-67, 1987 (PubMed).

Sanes, Engvall, Butkowski et al.: "Molecular heterogeneity of basal laminae: isoforms of laminin and collagen IV at the neuromuscular junction and elsewhere." in: The Journal of cell biology, Vol. 111, Issue 4, pp. 1685-99, 1990 (PubMed).

Sage, Woodbury, Bornstein: "Structural studies on human type IV collagen." in: The Journal of biological chemistry, Vol. 254, Issue 19, pp. 9893-900, 1979 (PubMed).