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Collagen, Type VII (COL7) antibody

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Synonyms COL7A1
(44), (25), (18), (2), (2), (2), (1), (1), (1), (1), (1)
(25), (18), (14)
Clonality (Clone)
Monoclonal ()
(3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Dot Blot (DB), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), ELISA, Western Blotting (WB)
(26), (25), (15), (14), (13), (11), (5), (5), (4), (3), (1), (1), (1), (1), (1)
Pubmed 7 references available
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Quantity 0.1 mL
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Immunogen Full length native protein (purified) (Human). Insoluble fractions prepared from neonatal foreskin epidermal cells.
Clone LH7-2
Isotype IgG1
Specificity Reactive in immunoblotting against intact tissue Type VII collagen (250kD). Pepsin digestion of terminal regions leads to complte loss of reactivity. Antibody reactivity is conserved after treatment with collagenase.No reactivity against collagen types I, II, III, IV, V, laminin and fibronectin.The antibody reacts predominantly with the basement membrane zone of stratified ssquamous epithelia and ectodermally derived glands.
Cross-Reactivity Cow (Bovine), Goat, Guinea Pig, Horse (Equine), Pig (Porcine), Sheep (Ovine)
Purification Unpurified. From mouse ascitic fluid.
Alternative Name Collagen type VII (COL7 Antibody Abstract)
Background This gene encodes the alpha chain of type VII collagen. The type VII collagen fibril, composed of three identical alpha collagen chains, is restricted to the basement zone beneath stratified squamous epithelia. It functions as an anchoring fibril between the external epithelia and the underlying stroma. Mutations in this gene are associated with all forms of dystrophic epidermolysis bullosa. In the absence of mutations, however, an acquired form of this disease can result from an autoimmune response made to type VII collagen. [provided by RefSeq]
Synonyms: EBR1, Collagen VII, 120120, 1294, Q02388, EBD1, COL7A1, EBDCT
Research Area Extracellular Matrix
Application Notes Dot: Use at an assay dependant dilution. ELISA: Use at an assay dependant dilution. ICC: Use at an assay dependant dilution. IF: Use at a dilution of 1/1000. (human or other mammalian frozen sections)IHC-Fr: Use at an assay dependant dilution. WB: Use at an assay dependant dilution. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Restrictions For Research Use only
Format Liquid
Buffer Ascites fluid containing 0.1% Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles.
Storage 4 °C
Storage Comment Keep as concentrated solution. Store at 4°C short term. For extended storage aliquot and store at -20°C or below.
Background publications Heagerty, Kennedy, Leigh et al.: "Identification of an epidermal basement membrane defect in recessive forms of dystrophic epidermolysis bullosa by LH 7:2 monoclonal antibody: use in diagnosis." in: The British journal of dermatology, Vol. 115, Issue 2, pp. 125-31, 1986 (PubMed).

Kirkham, Price, Gibson et al.: "Type VII collagen antibody LH 7.2 identifies basement membrane characteristics of thin malignant melanomas." in: The Journal of pathology, Vol. 157, Issue 3, pp. 243-7, 1989 (PubMed).

Wetzels, Holland, van Haelst et al.: "Detection of basement membrane components and basal cell keratin 14 in noninvasive and invasive carcinomas of the breast." in: The American journal of pathology, Vol. 134, Issue 3, pp. 571-9, 1989 (PubMed).

Shimizu, McDonald, Gunner et al.: "Epidermolysis bullosa acquisita antigen and the carboxy terminus of type VII collagen have a common immunolocalization to anchoring fibrils and lamina densa of basement membrane." in: The British journal of dermatology, Vol. 122, Issue 5, pp. 577-85, 1990 (PubMed).

Sanes, Engvall, Butkowski et al.: "Molecular heterogeneity of basal laminae: isoforms of laminin and collagen IV at the neuromuscular junction and elsewhere." in: The Journal of cell biology, Vol. 111, Issue 4, pp. 1685-99, 1990 (PubMed).

Fine, Horiguchi, Stein et al.: "Intraepidermal type VII collagen. Evidence for abnormal intracytoplasmic processing of a major basement membrane protein in rare patients with dominant and possibly localized recessive forms of dystrophic epidermolysis bullosa." in: Journal of the American Academy of Dermatology, Vol. 22, Issue 2 Pt 1, pp. 188-95, 1990 (PubMed).

Wetzels, Robben, Leigh et al.: "Distribution patterns of type VII collagen in normal and malignant human tissues." in: The American journal of pathology, Vol. 139, Issue 2, pp. 451-9, 1991 (PubMed).

Catalog No. ABIN145960
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