Cyclin-Dependent Kinase 4 (CDK4) antibody

Antigen: Cyclin-Dependent Kinase 4 (CDK4)

Synonyms: CMM3, PSK-J3, MGC14458, 8-6, CDK4, CDK4/6, DmCdk4, Pk53C, Pk?7, l(2)05428, l(2)0671, l(2)k06503, l(2)s4639, l(2)sh0671, DmelCG5072, CG5072, MGC133903

Clonality: Monoclonal

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)

Catalog no.: ABIN145970

Additional Information

Alternative name: CDK4

Immunogen: Recombinant full length protein (Human).

Cross-Reactivity: Human, Mouse (Murine), Rat (Rattus)

Isotype: IgG2a

Description: Regulation of cell cycle progression in eukaryotic cells depends on the expression of cyclins. These proteins form complexes with several different cyclin dependant kinases (CDKs). Within complexes, the cyclin subunit serves a regulatory role, whereas the CDKs have a catalytic protein kinase activity. Complexes of cyclins and CDKs play a key role in cell cycle control. The eukaryotic cell cycle is regulated by the sequential activation of CDKs.

Specificity: Reacts specifically with Cdk4. Does not recognise other Cdk types.

Application Details

Application Notes: ICC: Use at an assay dependant dilution. IP: Use at an assay dependant dilution. WB: Use at a dilution of 1/1000. (recognises a 33kD band, and it may pick up additional weaker bands, using a cultured human tumor cell line extract)Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

Purification: Ascites

Buffer: Ascitic fluid with 15mM sodium azide

Storage: Store at 4 C. Aliquot and store at -20 C long-term. Repeated freezing and thawing is not recommended. Storage in frost-free freezers is not recommended.

Research Area: Cell Cycle

Restrictions: For Research Use only

Publications

Matsushime, Ewen, Strom et al.: "Identification and properties of an atypical catalytic subunit (p34PSK-J3/cdk4) for mammalian D type G1 cyclins." in: Cell, Vol. 71, Issue 2, pp. 323-34, 1992 (PubMed).

Pines, Hunter: "Human cyclins A and B1 are differentially located in the cell and undergo cell cycle-dependent nuclear transport." in: The Journal of cell biology, Vol. 115, Issue 1, pp. 1-17, 1991 (PubMed).

Freeman, Donoghue: "Protein kinases and protooncogenes: biochemical regulators of the eukaryotic cell cycle." in: Biochemistry, Vol. 30, Issue 9, pp. 2293-302, 1991 (PubMed).

Lukas, Aagaard, Strauss et al.: "Oncogenic aberrations of p16INK4/CDKN2 and cyclin D1 cooperate to deregulate G1 control." in: Cancer research, Vol. 55, Issue 21, pp. 4818-23, 1995 (PubMed).

Aagaard, Lukas, Bartkova et al.: "Aberrations of p16Ink4 and retinoblastoma tumour-suppressor genes occur in distinct sub-sets of human cancer cell lines." in: International journal of cancer. Journal international du cancer, Vol. 61, Issue 1, pp. 115-20, 1995 (PubMed).

Lukas, Parry, Aagaard et al.: "Retinoblastoma-protein-dependent cell-cycle inhibition by the tumour suppressor p16." in: Nature, Vol. 375, Issue 6531, pp. 503-6, 1995 (PubMed).

Xiong, Zhang, Beach: "Subunit rearrangement of the cyclin-dependent kinases is associated with cellular transformation." in: Genes & development, Vol. 7, Issue 8, pp. 1572-83, 1993 (PubMed).

Motokura, Arnold: "Cyclins and oncogenesis." in: Biochimica et biophysica acta, Vol. 1155, Issue 1, pp. 63-78, 1993 (PubMed).

Sherr: "Mammalian G1 cyclins." in: Cell, Vol. 73, Issue 6, pp. 1059-65, 1993 (PubMed).

Elledge: "Cell cycle checkpoints: preventing an identity crisis." in: Science (New York, N.Y.), Vol. 274, Issue 5293, pp. 1664-72, 1997 (PubMed).