Cyclin-Dependent Kinase 4 (CDK4) antibody

Details for Product No. ABIN145970
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Antigen
Synonyms zgc:153726, CMM3, PSK-J3, Crk3, 8-6, CDK4, CDK4/6, CG5072, Cdk4/6, DmCdk4, Dmel\\CG5072, Pk53C, Pk?7, cdk, cdk4, cdk4/6, dCdk4, l(2)05428, l(2)0671, l(2)k06503, l(2)s4639, l(2)sh0671, xcdk4
Reactivity
Human
(170), (65), (48), (18), (13), (3), (1)
Host
Mouse
(96), (83), (5), (2), (1)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(6), (4), (4), (3), (3), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
(170), (58), (53), (38), (27), (16), (15), (10), (10), (9), (8), (5), (2), (2), (1)
Pubmed 10 references available
Quantity 0.1 mL
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Catalog No. ABIN145970
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Immunogen Recombinant full length protein (Human).
Clone DCS-31
Isotype IgG2a
Specificity Reacts specifically with Cdk4. Does not recognise other Cdk types.
Cross-Reactivity Mouse (Murine), Rat (Rattus)
Characteristics Cellular Localization: Cytoplasmic (likely)
Purification Ascites.
Alternative Name CDK4
Background Regulation of cell cycle progression in eukaryotic cells depends on the expression of cyclins. These proteins form complexes with several different cyclin dependant kinases (CDKs). Within complexes, the cyclin subunit serves a regulatory role, whereas the CDKs have a catalytic protein kinase activity. Complexes of cyclins and CDKs play a key role in cell cycle control. The eukaryotic cell cycle is regulated by the sequential activation of CDKs.
Synonyms: P11802, 1019, CDK4, 123829, PSKJ3, PSK-J3, CMM3, MGC14458, PSK J3
Research Area Cell Cycle
Application Notes ICC: Use at an assay dependant dilution. IP: Use at an assay dependant dilution. WB: Use at a dilution of 1/1000. (recognises a 33kD band, and it may pick up additional weaker bands, using a cultured human tumor cell line extract)Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Restrictions For Research Use only
Format Liquid
Buffer Ascitic fluid with 15mM sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles.
Storage 4 °C
Storage Comment Keep as concentrated solution. Store at 4°C short term. For extended storage aliquot and store at -20°C or below.
General Matsushime, Ewen, Strom et al.: "Identification and properties of an atypical catalytic subunit (p34PSK-J3/cdk4) for mammalian D type G1 cyclins." in: Cell, Vol. 71, Issue 2, pp. 323-34, 1992 (PubMed).

Pines, Hunter: "Human cyclins A and B1 are differentially located in the cell and undergo cell cycle-dependent nuclear transport." in: The Journal of cell biology, Vol. 115, Issue 1, pp. 1-17, 1991 (PubMed).

Freeman, Donoghue: "Protein kinases and protooncogenes: biochemical regulators of the eukaryotic cell cycle." in: Biochemistry, Vol. 30, Issue 9, pp. 2293-302, 1991 (PubMed).

Lukas, Aagaard, Strauss et al.: "Oncogenic aberrations of p16INK4/CDKN2 and cyclin D1 cooperate to deregulate G1 control." in: Cancer research, Vol. 55, Issue 21, pp. 4818-23, 1995 (PubMed).

Aagaard, Lukas, Bartkova et al.: "Aberrations of p16Ink4 and retinoblastoma tumour-suppressor genes occur in distinct sub-sets of human cancer cell lines." in: International journal of cancer. Journal international du cancer, Vol. 61, Issue 1, pp. 115-20, 1995 (PubMed).

Lukas, Parry, Aagaard et al.: "Retinoblastoma-protein-dependent cell-cycle inhibition by the tumour suppressor p16." in: Nature, Vol. 375, Issue 6531, pp. 503-6, 1995 (PubMed).

Xiong, Zhang, Beach: "Subunit rearrangement of the cyclin-dependent kinases is associated with cellular transformation." in: Genes & development, Vol. 7, Issue 8, pp. 1572-83, 1993 (PubMed).

Motokura, Arnold: "Cyclins and oncogenesis." in: Biochimica et biophysica acta, Vol. 1155, Issue 1, pp. 63-78, 1993 (PubMed).

Sherr: "Mammalian G1 cyclins." in: Cell, Vol. 73, Issue 6, pp. 1059-65, 1993 (PubMed).

Elledge: "Cell cycle checkpoints: preventing an identity crisis." in: Science (New York, N.Y.), Vol. 274, Issue 5293, pp. 1664-72, 1997 (PubMed).

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