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Coatomer (COPB) (AA 701-715) antibody

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AA 701-715
Clonality (Clone)
Monoclonal ()
Immunohistochemistry (Acetone-fixed) (IHC (af)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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Immunogen Synthetic peptide conjugated to KLH, corresponding to amino acids 701-715 of COPB.
Clone MaD
Isotype IgG1
Specificity Recognises an epitope in the COPB protein (110kD). The antibody stains the periphery of the Golgi complex in immunohistology using cultured cells.
Cross-Reactivity Hamster, Monkey, Rat (Rattus)
Purification Ascites.
Background This gene encodes a protein subunit of the coatomer complex associated with non-clathrin coated vesicles. The coatomer complex, also known as the coat protein complex 1, forms in the cytoplasm and is recruited to the Golgi by activated guanosine triphosphatases. Once at the Golgi membrane, the coatomer complex may assist in the movement of protein and lipid components back to the endoplasmic reticulum. Alternatively spliced transcript variants have been described. [provided by RefSeq]
Synonyms: 600959, beta COP, P53618, DKFZp761K102, COPB, 1315, FLJ10341, FLJ46444, FLJ57957
Application Notes AF: Use at an assay dependant dilution. IHC-F: Use at an assay dependant dilution. ICC: Use at an assay dependant dilution. IF: Use at a dilution of 1/80 using methanol/acetone or paraformaldehyde fixation, followed by a permeabilization step. Strong immunofluorescent staining is confined mainly to the central perinuclear area in the cell. IHC: Use at an assay dependant dilution. WB: Use at a dilution of 1/1000. May be used for micro-injection into cells. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Restrictions For Research Use only
Format Liquid
Buffer Preservative: 15mM Sodium Azide, Constituents: Ascites fluid
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles.
Storage 4 °C
Storage Comment Keep as concentrated solution. Store at 4°C short term. For extended storage aliquot and store at -20°C or below.
Supplier Images
 image for anti-Coatomer (COPB) (AA 701-715) antibody (ABIN146098) anti-Coatomer (COPB) (AA 701-715) antibody
Western Blotting (WB) image for anti-Coatomer (COPB) (AA 701-715) antibody (ABIN146098) anti-Coatomer (COPB) (AA 701-715) antibody (Image 2)
Background publications Kreis, Lowe, Pepperkok: "COPs regulating membrane traffic." in: Annual review of cell and developmental biology, Vol. 11, pp. 677-706, 1996 (PubMed).

Whitney, Gomez, Sheff et al.: "Cytoplasmic coat proteins involved in endosome function." in: Cell, Vol. 83, Issue 5, pp. 703-13, 1996 (PubMed).

Oprins, Duden, Kreis et al.: "Beta-COP localizes mainly to the cis-Golgi side in exocrine pancreas." in: The Journal of cell biology, Vol. 121, Issue 1, pp. 49-59, 1993 (PubMed).

Pepperkok, Scheel, Horstmann et al.: "Beta-COP is essential for biosynthetic membrane transport from the endoplasmic reticulum to the Golgi complex in vivo." in: Cell, Vol. 74, Issue 1, pp. 71-82, 1993 (PubMed).

Griffiths, Pepperkok, Locker et al.: "Immunocytochemical localization of beta-COP to the ER-Golgi boundary and the TGN." in: Journal of cell science, Vol. 108 ( Pt 8), pp. 2839-56, 1995 (PubMed).

Allan, Kreis: "A microtubule-binding protein associated with membranes of the Golgi apparatus." in: The Journal of cell biology, Vol. 103, Issue 6 Pt 1, pp. 2229-39, 1987 (PubMed).

Donaldson, Lippincott-Schwartz, Bloom et al.: "Dissociation of a 110-kD peripheral membrane protein from the Golgi apparatus is an early event in brefeldin A action." in: The Journal of cell biology, Vol. 111, Issue 6 Pt 1, pp. 2295-306, 1991 (PubMed).

Duden, Griffiths, Frank et al.: "Beta-COP, a 110 kd protein associated with non-clathrin-coated vesicles and the Golgi complex, shows homology to beta-adaptin." in: Cell, Vol. 64, Issue 3, pp. 649-65, 1991 (PubMed).

Ktistakis, Roth, Bloom: "PtK1 cells contain a nondiffusible, dominant factor that makes the Golgi apparatus resistant to brefeldin A." in: The Journal of cell biology, Vol. 113, Issue 5, pp. 1009-23, 1991 (PubMed).

Narula, McMorrow, Plopper et al.: "Identification of a 200-kD, brefeldin-sensitive protein on Golgi membranes." in: The Journal of cell biology, Vol. 117, Issue 1, pp. 27-38, 1992 (PubMed).