Coatomer (COPB) (Subunit beta) antibody

Details for Product No. ABIN146098
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Antigen
Epitope
Subunit beta
(3), (1), (1)
Reactivity
Hamster
(3), (2), (2), (2), (2), (1)
Host
Mouse
(3)
Clonality
Monoclonal
Application
Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB), Immunohistochemistry (IHC)
(3), (2), (1), (1)
Pubmed 10 references available
Catalog no. ABIN146098
Quantity 0.1 mL
Price
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Immunogen Synthetic peptide conjugated to KLH, corresponding to amino acids 701-715 of COP (beta).
Isotype IgG1
Specificity Recognises an epitope in the beta-COP protein (110kD). The antibody stains the periphery of the Golgi complex in immunohistology using cultured cells.
Cross-Reactivity Human, Monkey, Rat (Rattus)
Purification Ascites
Background Mouse monoclonal [maD] to beta COP
Application Notes AF: Use at an assay dependant dilution. IHC-F: Use at an assay dependant dilution. ICC: Use at an assay dependant dilution. IF: Use at a dilution of 1/80 using methanol/acetone or paraformaldehyde fixation, followed by a permeabilization step. Strong immunofluorescent staining is confined mainly to the central perinuclear area in the cell. IHC: Use at an assay dependant dilution. WB: Use at a dilution of 1/1000. May be used for micro-injection into cells. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Restrictions For Research Use only
Format Liquid
Buffer Ascites fluid
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
General Narula, McMorrow, Plopper et al.: "Identification of a 200-kD, brefeldin-sensitive protein on Golgi membranes." in: The Journal of cell biology, Vol. 117, Issue 1, pp. 27-38, 1992 (PubMed).

Ktistakis, Roth, Bloom: "PtK1 cells contain a nondiffusible, dominant factor that makes the Golgi apparatus resistant to brefeldin A." in: The Journal of cell biology, Vol. 113, Issue 5, pp. 1009-23, 1991 (PubMed).

Duden, Griffiths, Frank et al.: "Beta-COP, a 110 kd protein associated with non-clathrin-coated vesicles and the Golgi complex, shows homology to beta-adaptin." in: Cell, Vol. 64, Issue 3, pp. 649-65, 1991 (PubMed).

Donaldson, Lippincott-Schwartz, Bloom et al.: "Dissociation of a 110-kD peripheral membrane protein from the Golgi apparatus is an early event in brefeldin A action." in: The Journal of cell biology, Vol. 111, Issue 6 Pt 1, pp. 2295-306, 1991 (PubMed).

Allan, Kreis: "A microtubule-binding protein associated with membranes of the Golgi apparatus." in: The Journal of cell biology, Vol. 103, Issue 6 Pt 1, pp. 2229-39, 1987 (PubMed).

Griffiths, Pepperkok, Locker et al.: "Immunocytochemical localization of beta-COP to the ER-Golgi boundary and the TGN." in: Journal of cell science, Vol. 108 ( Pt 8), pp. 2839-56, 1995 (PubMed).

Pepperkok, Scheel, Horstmann et al.: "Beta-COP is essential for biosynthetic membrane transport from the endoplasmic reticulum to the Golgi complex in vivo." in: Cell, Vol. 74, Issue 1, pp. 71-82, 1993 (PubMed).

Oprins, Duden, Kreis et al.: "Beta-COP localizes mainly to the cis-Golgi side in exocrine pancreas." in: The Journal of cell biology, Vol. 121, Issue 1, pp. 49-59, 1993 (PubMed).

Whitney, Gomez, Sheff et al.: "Cytoplasmic coat proteins involved in endosome function." in: Cell, Vol. 83, Issue 5, pp. 703-13, 1996 (PubMed).

Kreis, Lowe, Pepperkok: "COPs regulating membrane traffic." in: Annual review of cell and developmental biology, Vol. 11, pp. 677-706, 1996 (PubMed).

Hosts (3)
Reactivities (3), (2), (2), (2), (2), (1)
Applications (3), (2), (1), (1)
Epitopes (3), (1), (1)
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

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Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
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