Coatomer (COPB) (Subunit beta) antibody

Antigen: Coatomer (COPB)

Binding Site: Subunit beta

Clonality: Monoclonal

Host: Mouse

Reactivity: Hamster

Application: Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB), Immunohistochemistry (IHC)

Catalog no.: ABIN146098

Additional Information

Immunogen: Synthetic peptide conjugated to KLH, corresponding to amino acids 701-715 of COP (beta).

Cross-Reactivity: Hamster, Human, Monkey, Rat (Rattus)

Isotype: IgG1

Description: Mouse monoclonal [maD] to beta COP

Specificity: Recognises an epitope in the beta-COP protein (110kD). The antibody stains the periphery of the Golgi complex in immunohistology using cultured cells.

Application Details

Application Notes: AF: Use at an assay dependant dilution. IHC-F: Use at an assay dependant dilution. ICC: Use at an assay dependant dilution. IF: Use at a dilution of 1/80 using methanol/acetone or paraformaldehyde fixation, followed by a permeabilization step. Strong immunofluorescent staining is confined mainly to the central perinuclear area in the cell. IHC: Use at an assay dependant dilution. WB: Use at a dilution of 1/1000. May be used for micro-injection into cells. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

Purification: Ascites

Buffer: Ascites fluid

Preservative: 15mM Sodium Azide

Storage: Store at 4 C. For extended storage freeze in working aliquots. Repeated freezing and thawing is not recommended. Storage in frost-free freezers is not recommended.

Restrictions: For Research Use only

Publications

Narula, McMorrow, Plopper et al.: "Identification of a 200-kD, brefeldin-sensitive protein on Golgi membranes." in: The Journal of cell biology, Vol. 117, Issue 1, pp. 27-38, 1992 (PubMed).

Ktistakis, Roth, Bloom: "PtK1 cells contain a nondiffusible, dominant factor that makes the Golgi apparatus resistant to brefeldin A." in: The Journal of cell biology, Vol. 113, Issue 5, pp. 1009-23, 1991 (PubMed).

Duden, Griffiths, Frank et al.: "Beta-COP, a 110 kd protein associated with non-clathrin-coated vesicles and the Golgi complex, shows homology to beta-adaptin." in: Cell, Vol. 64, Issue 3, pp. 649-65, 1991 (PubMed).

Donaldson, Lippincott-Schwartz, Bloom et al.: "Dissociation of a 110-kD peripheral membrane protein from the Golgi apparatus is an early event in brefeldin A action." in: The Journal of cell biology, Vol. 111, Issue 6 Pt 1, pp. 2295-306, 1991 (PubMed).

Allan, Kreis: "A microtubule-binding protein associated with membranes of the Golgi apparatus." in: The Journal of cell biology, Vol. 103, Issue 6 Pt 1, pp. 2229-39, 1987 (PubMed).

Griffiths, Pepperkok, Locker et al.: "Immunocytochemical localization of beta-COP to the ER-Golgi boundary and the TGN." in: Journal of cell science, Vol. 108 ( Pt 8), pp. 2839-56, 1995 (PubMed).

Pepperkok, Scheel, Horstmann et al.: "Beta-COP is essential for biosynthetic membrane transport from the endoplasmic reticulum to the Golgi complex in vivo." in: Cell, Vol. 74, Issue 1, pp. 71-82, 1993 (PubMed).

Oprins, Duden, Kreis et al.: "Beta-COP localizes mainly to the cis-Golgi side in exocrine pancreas." in: The Journal of cell biology, Vol. 121, Issue 1, pp. 49-59, 1993 (PubMed).

Whitney, Gomez, Sheff et al.: "Cytoplasmic coat proteins involved in endosome function." in: Cell, Vol. 83, Issue 5, pp. 703-13, 1996 (PubMed).

Kreis, Lowe, Pepperkok: "COPs regulating membrane traffic." in: Annual review of cell and developmental biology, Vol. 11, pp. 677-706, 1996 (PubMed).