Tumor Necrosis Factor (Ligand) Superfamily, Member 11 (TNFSF11) antibody

Antigen: Tumor Necrosis Factor (Ligand) Superfamily, Member 11 (TNFSF11)

Synonyms: ODF, OPGL, sOdf, CD254, OPTB2, RANKL, TRANCE, hRANKL2, TNFSF11

Clonality: Monoclonal

Host: Mouse

Reactivity: Mouse (Murine)

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Catalog no.: ABIN146331

Additional Information

Alternative name: CD254 / RANKL

Immunogen: Fusion protein, corresponding to amino acids 1-317 of Mouse sRANKL.

Cross-Reactivity: Mouse (Murine)

Isotype: IgG1

Description: SRANKL is a member of the tumor necrosis factor (TNF) cytokine family which is a ligand for osteoprotegerin and functions as a key factor for osteoclast differentiation and activation. This protein was shown to be a dentritic cell survival factor and is involved in the regulation of T cell-dependent immune response. T cell activation was reported to induce expression of this gene and lead to an increase of osteoclastogenesis and bone loss. This protein was shown to activate antiapoptotic kinase AKT/PKB through a signaling complex involving SRC kinase and tumor necrosis factor receptor-associated factor (TRAF) 6, which indicated this protein may have a role in the regulation of cell apoptosis.

Application Details

Application Notes: IHC: Use at an assay dependent dilution. WB: Use at a concentration of 1-2 ug/ml. Detects a band of approximately 35 kDa. Is unsuitable for FACS. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

Concentration: 0.5 mg/ml

Purification: Protein G affinity purified

Buffer: PBS

Preservative: 0.02% Sodium Azide

Storage: Store at 4 C. Aliquot and store at -20 C long-term.

Restrictions: For Research Use only

Images

anti-Tumor Necrosis Factor (Ligand) Superfamily, Member 11 (TNFSF11) antibody

Publications

Hsu, Lacey, Dunstan et al.: "Tumor necrosis factor receptor family member RANK mediates osteoclast differentiation and activation induced by osteoprotegerin ligand." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 96, Issue 7, pp. 3540-5, 1999 (PubMed).

Kikuchi, Matsuguchi, Tsuboi et al.: "Gene expression of osteoclast differentiation factor is induced by lipopolysaccharide in mouse osteoblasts via Toll-like receptors." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 166, Issue 5, pp. 3574-9, 2001 (PubMed).

Pearse, Sordillo, Yaccoby et al.: "Multiple myeloma disrupts the TRANCE/ osteoprotegerin cytokine axis to trigger bone destruction and promote tumor progression." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 98, Issue 20, pp. 11581-6, 2001 (PubMed).

Fiumara, Snell, Li et al.: "Functional expression of receptor activator of nuclear factor kappaB in Hodgkin disease cell lines." in: Blood, Vol. 98, Issue 9, pp. 2784-90, 2001 (PubMed).