Fem-1 Homolog B (C. Elegans) (FEM1B) antibody

Antigen: Fem-1 Homolog B (C. Elegans) (FEM1B)

Synonyms: FIAA, DKFZp451E0710, MGC80444, FEM1B

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB)

Catalog no.: ABIN147551

Additional Information

Alternative name: FEM1B

Immunogen: Available on request

Cross-Reactivity: Human, Mouse (Murine), Rat (Rattus)

Isotype: IgG

Description: F1A alpha, also termed FEM1 beta, is a novel protein that associates with the intracellular domains of Fas and TNFR1. Over expression of F1A alpha induces apoptosis. F1A alpha also associates with CED-4 and its mammalian homologue Apaf-1, and can be cleaved by CED-3 and caspases.

Application Details

Application Notes: WB: Use at a concentration of 1-2 ug/ml. A 70kDa band is observed when using mouse liver tissue lysate. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

Concentration: 0.5 mg/ml

Purification: Immunogen affinity purified

Buffer: PBS pH 7.2 with 50% glycerol

Preservative: 1% BSA and 0.02% sodium azide

Storage: Store at -20 C. For long term storage, aliquot and refreeze at -70 C. Avoid repeated freeze/defrost cycles

Restrictions: For Research Use only

Publications

Lefkowitz: "Identification of adenylate cyclase-coupled beta-adrenergic receptors with radiolabeled beta-adrenergic antagonists." in: Biochemical pharmacology, Vol. 24, Issue 18, pp. 1651-8, 1976 (PubMed).

Chan, Tan, Zhang et al.: "F1Aalpha, a death receptor-binding protein homologous to the Caenorhabditis elegans sex-determining protein, FEM-1, is a caspase substrate that mediates apoptosis." in: The Journal of biological chemistry, Vol. 274, Issue 45, pp. 32461-8, 1999 (PubMed).