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Polynucleotide Kinase 3'-Phosphatase (PNKP) (C-Term) antibody

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Antigen
Synonyms Tb06.28P18.320, 21.m03011, 1810009G08Rik, PNK, EIEE10, MCSZ, C21orf124, C21orf97, PKH
Alternatives
Epitope
C-Term
(20), (15), (10), (7), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Reactivity
Human
(61), (4), (3), (3), (3), (1), (1)
Host
Goat
(50), (9), (3)
Clonality
Polyclonal
Conjugate
Un-conjugated
(2), (2), (2), (2), (2), (2)
Application
Western Blotting (WB)
(56), (33), (10), (9), (7), (2), (1)
Pubmed 1 reference available
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Quantity 50 μg
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Immunogen Peptide with sequence EPRLGRLYCQFSEG, from C Terminus of the protein sequence according to NP_009185.
Isotype IgG
Purification IgG fraction. Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide..
Alternative Name PNKP (PNKP Antibody Abstract)
Background Mammalian PNK catalyzes the 5-prime phosphorylation of nucleic acids and can have associated 3-prime phosphatase activity, predictive of an important function in DNA repair following ionizing radiation or oxidative damage.
Synonyms: EIEE10, MCSZ, PNK
Pathways DNA Damage Repair
Application Notes WB on fusion protein: Use at a dilution of 1/1000. This antibody has been used to successfully detect PNK as a fusion protein in a yeast lysate (see picture below). However, it has not been successfully used to detect endogenous PNK in cell lines (HepG2, HeLa and 293). Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user. PNK is detected at 60kD in Western blotting.
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer Tris saline, 0.02% Na azide, pH7.3 with 0.5% BSA
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles.
Storage -20 °C
Storage Comment Upon receipt - Keep as concentrated solution. Aliquot and store at -20°C or below.
Supplier Images
Image no. 1 for anti-Polynucleotide Kinase 3'-Phosphatase (PNKP) (C-Term) antibody (ABIN147848) anti-Polynucleotide Kinase 3'-Phosphatase (PNKP) (C-Term) antibody
Background publications Jilani, Ramotar, Slack et al.: "Molecular cloning of the human gene, PNKP, encoding a polynucleotide kinase 3'-phosphatase and evidence for its role in repair of DNA strand breaks caused by oxidative damage." in: The Journal of biological chemistry, Vol. 274, Issue 34, pp. 24176-86, 1999 (PubMed).

Catalog No. ABIN147848
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