Polynucleotide Kinase 3'-Phosphatase (PNKP) antibody

Details for Product No. ABIN147848
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Antigen
Synonyms EIEE10, MCSZ, PNK, 1810009G08Rik, Tb06.28P18.320, 21.m03011
Reactivity
Human
(25), (5), (5), (3), (1)
Host
Goat
(22), (4), (3)
Clonality
Polyclonal
Application
Western Blotting (WB)
(28), (12), (6), (4), (2), (1), (1), (1)
Pubmed 1 reference available
Catalog no. ABIN147848
Quantity 50 µg
Price
Contact our Customer Service for availability and price in your country.
Shipping to United States (Change)
Add to Basket

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen Peptide with sequence EPRLGRLYCQFSEG, from C Terminus of the protein sequence according to NP_009185.
Isotype IgG
Purification Ig Fraction
Alternative Name PNKP
Background Mammalian PNK catalyzes the 5-prime phosphorylation of nucleic acids and can have associated 3-prime phosphatase activity, predictive of an important function in DNA repair following ionizing radiation or oxidative damage.
Application Notes WB on fusion protein: Use at a dilution of 1/1000. This antibody has been used to successfully detect PNK as a fusion protein in a yeast lysate (see picture below). However, it has not been successfully used to detect endogenous PNK in cell lines (HepG2, HeLa and 293). Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user. PNK is detected at 60kD in Western blotting.
Restrictions For Research Use only
Concentration 0.5 mg/ml
Buffer Tris saline, 0.02% Na azide, pH7.3 with 0.5% BSA
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Supplier Images
anti-Polynucleotide Kinase 3'-Phosphatase (PNKP) antibody anti-Polynucleotide Kinase 3'-Phosphatase (PNKP) antibody
General Jilani, Ramotar, Slack et al.: "Molecular cloning of the human gene, PNKP, encoding a polynucleotide kinase 3'-phosphatase and evidence for its role in repair of DNA strand breaks caused by oxidative damage." in: The Journal of biological chemistry, Vol. 274, Issue 34, pp. 24176-86, 1999 (PubMed).

Hosts (22), (4), (3)
Reactivities (25), (5), (5), (3), (1)
Applications (28), (12), (6), (4), (2), (1), (1), (1)
Epitopes (6), (4), (3), (2), (2), (1), (1), (1), (1)
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Add to Basket

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Validation Images
back to top