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Details for Product No. ABIN147848

Polynucleotide Kinase 3'-Phosphatase (PNKP) antibody

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Antigen
Synonyms EIEE10, MCSZ, PNK, 1810009G08Rik, Tb06.28P18.320, 21.m03011
Reactivity
»Alternatives Human
Host
Clonality Polyclonal
Application
»Alternatives Western Blotting (WB)
Pubmed 1 reference available
Catalog no. ABIN147848
Quantity 0.1 mg
Price
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Immunogen Peptide with sequence EPRLGRLYCQFSEG, from C Terminus of the protein sequence according to NP_009185.
Isotype IgG
Purification Ig Fraction
Alternative Name PNKP
Background Mammalian PNK catalyzes the 5-prime phosphorylation of nucleic acids and can have associated 3-prime phosphatase activity, predictive of an important function in DNA repair following ionizing radiation or oxidative damage.
Application Notes WB on fusion protein: Use at a dilution of 1/1000. This antibody has been used to successfully detect PNK as a fusion protein in a yeast lysate (see picture below). However, it has not been successfully used to detect endogenous PNK in cell lines (HepG2, HeLa and 293). Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user. PNK is detected at 60kD in Western blotting.
Restrictions For Research Use only
Concentration 0.5 mg/ml
Buffer Tris saline, 0.02% Na azide, pH7.3 with 0.5% BSA
Preservative Sodium azide
Storage -20 °C
Jilani, Ramotar, Slack et al.: "Molecular cloning of the human gene, PNKP, encoding a polynucleotide kinase 3'-phosphatase and evidence for its role in repair of DNA strand breaks caused by oxidative damage." in: The Journal of biological chemistry, Vol. 274, Issue 34, pp. 24176-86, 1999 (PubMed).

Alternatives for antigen "Polynucleotide Kinase 3'-Phosphatase (PNKP)", type "Antibodies"
Hosts (29), (6), (2)
Reactivities (32), (5), (5), (4), (1)
Applications (33), (14), (6), (5), (2), (1), (1), (1), (1), (1)
Epitopes (6), (5), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1)
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