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Ribosomal Protein S6 Kinase, 90kDa, Polypeptide 5 (RPS6KA5) (pThr581) antibody

Antigen

Ribosomal Protein S6 Kinase, 90kDa, Polypeptide 5 (RPS6KA5)
Synonyms MSK1, RLPK, MSPK1, MGC1911
Binding Site
Alternatives

pThr581
Clonality Polyclonal
Host
Alternatives

Rabbit
Reactivity
Alternatives

Human
Conjugate
Alternatives Un-conjugated
Application
Alternatives Western Blotting (WB)
2 references available
Catalog no. ABIN148003
Quantity 50 µg  (0.5 mg/ml)
Price Product not available in this region.
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Additional Information
Application Details Publications Alternatives back to top

Alternative name RPS6KA5 / MSK1
Immunogen Available on request.
Cross-Reactivity Human, Mouse (Murine)
Isotype IgG
Description MSK 1, a Mitogen and Stress activated protein Kinase, is activated by ERK as well as p38 MAPK in response to growth factors and various cellular stress stimuli. MSK 1 resembles RSK by having two kinase domains connected by a regulator linker region. Phosphorylation of RSK 1 at Ser 364, Ser381 and Thr574 is critical for RSK 1 activity. These sites are analogous to Ser360, Ser376 and Thr581 of MSK 1 and maybe important for MSK 1 activity as well.
Specificity This antibody detects 90 kDa MSK 1 only when phosphorylated at Thr581. It does not cross-react with non-phosphorylated MSK 1, or MSK 1 phosphorylated at other sites. It does not cross-react with RSK 1, RSK 2 or RSK 3.

Application Details
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Application Notes WB: Use at a concentration of 1-2 ug/ml. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Concentration 0.5 mg/ml
Purification Immunogen affinity purified
Buffer PBS pH 7.2 with 50% glycerol
Preservative 1% BSA and 0.02% sodium azide
Storage Store at -20 C. For long term storage, aliquot and refreeze at -70 C. Avoid repeated freeze/defrost cycles
Restrictions For Research Use only

Publications
Application Details Alternatives back to top

Ardenne, Reitnauer: "[Tumour hyperacidulation through intravenous glucose infusion enhanced by amygdalin and beta-glucosidase application (author's transl)]" in: Archiv für Geschwulstforschung, Vol. 45, Issue 2, pp. 135-45, 1976 (PubMed).

Deak, Clifton, Lucocq et al.: "Mitogen- and stress-activated protein kinase-1 (MSK1) is directly activated by MAPK and SAPK2/p38, and may mediate activation of CREB." in: The EMBO journal, Vol. 17, Issue 15, pp. 4426-41, 1998 (PubMed).

Alternatives
Application Details Publications back to top

Alternatives for antigen "Ribosomal Protein S6 Kinase, 90kDa, Polypeptide 5 (RPS6KA5)", type "Antibodies"
Hosts Rabbit (84), Mouse (2), Goat (1)
Reactivities Human (86), Mouse (Murine) (64), Rat (Rattus) (42), Dog (Canine) (38), Chicken (33), Cow (Bovine) (33), Pig (Porcine) (33), Horse (Equine) (23)
Applications Immunofluorescence (IF) (48), Western Blotting (WB) (47), ELISA (28), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (12), Immunohistochemistry (IHC) (11), Immunocytochemistry (ICC) (5), Immunoprecipitation (IP) (4), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Immunoelectron Microscopy (IEM) (1)
Conjugates Alexa Fluor 350 (5), Alexa Fluor 488 (5), Alexa Fluor 555 (5), Alexa Fluor 647 (5), PE,Cy5.5 (5), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy7 (1)
Epitopes pSer376 (9), pSer360 (8), pThr581 (7), pSer212 (6), Internal Region (4), AA 1-802 (1), C-Term (1), Center (1), pSer281,pThr277 (1), pThr581,Phosphospecific (1)