Mechanistic Target of Rapamycin (serine/threonine Kinase) (FRAP1) (pSer2448) antibody

Details for Product No. ABIN148190
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Antigen
Synonyms FRAP, FRAP1, FRAP2, RAFT1, RAPT1, 2610315D21Rik, AI327068, Frap1, flat, frap1, tor, wu:fc22h08
Epitope
pSer2448
(45), (22), (21), (12), (7), (6), (6), (5), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(243), (153), (145), (63), (36), (24), (5), (2), (1)
Host
Rabbit
(241), (13), (1)
Clonality
Polyclonal
Conjugate
Un-conjugated
(6), (6), (5), (5), (5), (5), (5), (5), (5), (5), (5)
Application
Western Blotting (WB)
(144), (85), (73), (64), (50), (34), (22), (17), (4), (3), (2), (1), (1)
Pubmed 2 references available
Catalog no. ABIN148190
Quantity 0.1 mg
Price
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  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen Synthetic peptide: KRSRTRTDpSYSAGQSVEI (Human).
Isotype IgG
Specificity Specific for phospho mTOR, giving a band at approx 250 kD.
Purification Immunogen affinity purified
Alternative Name MTOR / FRAP1
Background FKBP12-rapamycin associated protein (FRAP) is one of a family of proteins involved in cell cycle progression, DNA recombination, and DNA damage detection. In rat, it is a 245-kD protein (symbolized RAFT1) with significant homology to the Saccharomyces cerevisiae protein TOR1 and has been shown to associate with the immunophilin FKBP12 in a rapamycin-dependent fashion (1). The FKBP12-rapamycin complex is known to inhibit progression through the G1 cell cycle stage by interfering with mitogenic signaling pathways involved in G1 progression in several cell types, as well as in yeast (2). The binding of FRAP to FKBP12-rapamycin correlated with the ability of these ligands to inhibit cell cycle progression (2).
Application Notes Western blot: use at 1/1000This product has been assayed by ELISA against 0.1ug of the immunizing peptide. A 1/6000-1/29,000 dilution of the antibody is recommended. Optimal dilutions should be determined by the end user.
Restrictions For Research Use only
Concentration 1 mg/ml
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
General Neviackas, Kerstein: "Pancreatic enzyme response with an elemental diet." in: Surgery, gynecology & obstetrics, Vol. 142, Issue 1, pp. 71-4, 1976 (PubMed).

Sabatini, Erdjument-Bromage, Lui et al.: "RAFT1: a mammalian protein that binds to FKBP12 in a rapamycin-dependent fashion and is homologous to yeast TORs." in: Cell, Vol. 78, Issue 1, pp. 35-43, 1994 (PubMed).

Hosts (241), (13), (1)
Reactivities (243), (153), (145), (63), (36), (24), (5), (2), (1)
Applications (144), (85), (73), (64), (50), (34), (22), (17), (4), (3), (2), (1), (1)
Conjugates (6), (6), (5), (5), (5), (5), (5), (5), (5), (5), (5)
Epitopes (45), (22), (21), (12), (7), (6), (6), (5), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Add to Basket

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
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