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MTOR antibody (Mechanistic Target of Rapamycin (serine/threonine Kinase)) (pSer2448)

Details for Product anti-FRAP1 Antibody No. ABIN148190, Supplier: Login to see
Antigen
  • FRAP1
  • FRAP
  • FRAP2
  • RAFT1
  • RAPT1
  • 2610315D21Rik
  • AI327068
  • Frap1
  • flat
  • frap1
  • tor
  • wu:fc22h08
Alternatives
anti-Human MTOR antibody for Western Blotting
Epitope
pSer2448
66
25
24
23
21
21
15
13
13
9
7
6
4
3
3
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
340
159
147
7
2
2
2
2
1
1
Host
Rabbit
294
47
2
Clonality
Polyclonal
Conjugate
This MTOR antibody is un-conjugated
7
7
7
5
4
4
4
4
4
4
4
4
4
3
3
1
1
1
1
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
222
112
87
67
55
45
33
22
8
8
7
3
2
2
2
2
2
1
Supplier
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Immunogen Synthetic peptide: KRSRTRTDpSYSAGQSVEI (Human).
Isotype IgG
Specificity Specific for phospho mTOR, giving a band at approx 250 kD.
Purification Immunogen affinity purified.
Alternative Name MTOR / FRAP1 (FRAP1 Antibody Abstract)
Background FKBP12-rapamycin associated protein (FRAP) is one of a family of proteins involved in cell cycle progression, DNA recombination, and DNA damage detection. In rat, it is a 245-kD protein (symbolized RAFT1) with significant homology to the Saccharomyces cerevisiae protein TOR1 and has been shown to associate with the immunophilin FKBP12 in a rapamycin-dependent fashion (1). The FKBP12-rapamycin complex is known to inhibit progression through the G1 cell cycle stage by interfering with mitogenic signaling pathways involved in G1 progression in several cell types, as well as in yeast (2). The binding of FRAP to FKBP12-rapamycin correlated with the ability of these ligands to inhibit cell cycle progression (2).
Synonyms: RAPT1, 601231, 2475, P42345, MTOR, FRAP, FRAP2, FRAP1, RAFT1, mTOR (phospho S2448), mTOR (phospho Ser2448), mTOR (pS2448), mTOR (pSer2448), mTOR phospho S2448, mTOR phospho Ser2448, Phospho mTOR (S2448), Phospho mTOR (Ser2448), Phospho mTOR (pS2448), Phospho mTOR (pSer2448)
Pathways PI3K-Akt Signaling, RTK Signaling, AMPK Signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway
Application Notes Western blot: use at 1/1000This product has been assayed by ELISA against 0.1ug of the immunizing peptide. A 1/6000-1/29,000 dilution of the antibody is recommended. Optimal dilutions should be determined by the end user.
Restrictions For Research Use only
Format Liquid
Concentration 1.88 mg/mL
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, 0.01% Sodium Azide, pH 7.2
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles.
Storage -20 °C
Storage Comment Upon receipt - Keep as concentrated solution. Aliquot and store at -20°C or below.
Supplier Images
Immunohistochemistry (IHC) image for anti-MTOR antibody (Mechanistic Target of Rapamycin (serine/threonine Kinase)) (pSer2448) (ABIN148190) anti-Mechanistic Target of Rapamycin (serine/threonine Kinase) (FRAP1) (pSer2448) antibody
Western Blotting (WB) image for anti-MTOR antibody (Mechanistic Target of Rapamycin (serine/threonine Kinase)) (pSer2448) (ABIN148190) anti-Mechanistic Target of Rapamycin (serine/threonine Kinase) (FRAP1) (pSer2448) antibody (Image 2)
Background publications Sabatini, Erdjument-Bromage, Lui et al.: "RAFT1: a mammalian protein that binds to FKBP12 in a rapamycin-dependent fashion and is homologous to yeast TORs." in: Cell, Vol. 78, Issue 1, pp. 35-43, 1994 (PubMed).

Neviackas, Kerstein: "Pancreatic enzyme response with an elemental diet." in: Surgery, gynecology & obstetrics, Vol. 142, Issue 1, pp. 71-4, 1976 (PubMed).