SH3-Domain GRB2-Like Endophilin B1 (SH3GLB1) antibody

Antigen: SH3-Domain GRB2-Like Endophilin B1 (SH3GLB1)

Synonyms: Bif-1, CGI-61, KIAA0491, dJ612B15.2, im:5777505, wu:fa08f03, wu:fb13e12, MGC79086, SH3GLB1, sh3glb1, MGC110259, wu:fc55h11, wu:fj98d09, DKFZp469C246

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: Western Blotting (WB)

Catalog no.: ABIN148611

Additional Information

Alternative name: Endophilin-B1

Immunogen: Peptide-amino acids QKGKVPITYLELLN, from C-Terminus of Bif according to NP_057093

Cross-Reactivity: Human, Mouse (Murine)

Isotype: IgG

Description: The Bcl2 family consists of anti-apoptotic (or cell survival) genes, such as Bcl2, BclXL and pro-apoptotic (or cell death) genes including Bax and BAD. In addition to its anti-apoptotic effect, Bcl2 also inhibits progression through the cell cycle. Functional interactions between family members as well as binding to other cellular proteins modulate their activities. Bcl2 family members are important in normal tissue development, homeostasis, and disease states. Bcl2 prevents cell death by forming heterodimers with Bax and Bad. Bax promotes cell death by forming homodimers, which can be blocked by forming heterodimers with Bcl2 or BCL-XL. Recently, using yeast two-hybrid system it has been identified a cDNA encoding a 365 amino acid protein, which interacts physically with Bax protein and influences cell life and death. Bif1 contains a Src homology 3 (SH3) domain in the C-terminus. It does not contain any of the conserved Bcl2 homology (BH) domains of the Bcl2 family proteins. Human

Application Details

Application Notes: For WB: Use at a concentration of 0.2-1.0ug/ml. Detects a band at approximately 40kD. 50kD bands seen in Jurkat cell lysate. Please note that 50kDa band could represent another isoform of BIF-1 (AAF73017), which also has several consensus glycosylation sites. Glycosylation can affect migration of proteins on SDS-PAGE [Hames, B.D and Rickwood, D., eds. Gel electrophoresis of proteins: a practical approach. Oxford University Press].

Concentration: 0.5 mg/ml

Purification: Serum

Buffer: Tris saline, 0.02% Na azide, pH7.3 containing 0.5% BSA

Storage: Keep as concentrated solution. Aliquot and store at -20?C or below. Avoid freeze-thaw cycles.

Restrictions: For Research Use only

Images

anti-SH3-Domain GRB2-Like Endophilin B1 (SH3GLB1) antibody

Publications

Pierrat, Simonen, Cueto et al.: "SH3GLB, a new endophilin-related protein family featuring an SH3 domain." in: Genomics, Vol. 71, Issue 2, pp. 222-34, 2001 (PubMed).